In:
The Journal of Immunology, The American Association of Immunologists, Vol. 182, No. 1_Supplement ( 2009-04-01), p. 131.11-131.11
Abstract:
Dendritic cells (DCs) can induce and control immune responses. Types of pre-existing memory CD4+ T cells can influence the efficacy of vaccines against influenza viruses. We here show that quality of influenza-specific CD4+ T cells can be modulated by delivering antigens to subsets of human DCs via Dectin-1. A major antigen for neutralizing antibodies, HA1 of hemagglutinin (H1), was delivered to DCs by using recombinant fusion proteins, anti-Dectin-1-HA1. First, all healthy individuals tested had relatively potent HA1-specific Th1 and Th2 helper T cells. However, magnitudes of IL-10-, IL-17-, and IL-21-producing were variable in individuals and by peptide epitopes presented by DCs. Second, anti-Dectin-1-HA1 induced DCs to secrete IL-23 that upregulated IFNγ and IL-17, but downregulated IL-13. CD70 upregulated by anti-Dectin-1-HA1 contributed to the expression of IFNγ, IL-17, IL-10, and IL-13, but not IL-21. Third, lipopolysaccharide from P. gingivalis, not E. coli, permitted myeloid DCs (mDCs) to promote Th1 and Th17, but suppress Th2 type responses, in CD70-/IL-1β-dependent manners. Lastly, plasmacytoid DCs (pDCs) and mDCs expressed same variants of Dectin-1 and they presented same peptide epitopes. However, mDCs were more potent than pDCs for inducing cytokines tested, particularly IFNγ and IL-13. Thus vaccines that target Dectin-1 have a potential to enhance the immunity against influenza infections.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.182.Supp.131.11
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2009
detail.hit.zdb_id:
1475085-5
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