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  • 1
    Online Resource
    Online Resource
    Molecular Cloning and mRNA Expression of the Porcine Insulin-responsive Glucose Transporter (GLUT4)
    Asian Australasian Association of Animal Production Societies ; 2010
    In:  Asian-Australasian Journal of Animal Sciences Vol. 23, No. 5 ( 2010-04-22), p. 640-648
    Details
    In: Asian-Australasian Journal of Animal Sciences, Asian Australasian Association of Animal Production Societies, Vol. 23, No. 5 ( 2010-04-22), p. 640-648
    Type of Medium: Online Resource
    ISSN: 1011-2367 , 1976-5517
    URL: Article
    DOI: 10.5713/ajas.2010.90313
    Language: English
    Publisher: Asian Australasian Association of Animal Production Societies
    Publication Date: 2010
    detail.hit.zdb_id: 2727534-6
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  • 2
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    MicroRNA‐27a inhibits porcine type I muscle fibre gene expression by directly targeting peroxisome proliferator‐activated receptor‐γ coactivator‐1α
    Wiley ; 2023
    In:  Journal of Animal Physiology and Animal Nutrition Vol. 107, No. 4 ( 2023-07), p. 1054-1064
    Details
    In: Journal of Animal Physiology and Animal Nutrition, Wiley, Vol. 107, No. 4 ( 2023-07), p. 1054-1064
    Abstract: Skeletal muscle is a heterogeneous tissue comprising of physiologically and biochemically diverse muscle fibres, which play a key role in affecting physicochemical properties of skeletal muscle. Although it is well‐known about the transcriptional control of muscle fibre gene expression in animals through certain signalling pathways, the posttranscriptional regulation of it in pigs remains poorly understood. In this study, through bioinformatics analysis, transfection analysis and dual‐luciferase reporter analysis, we found that miR‐27a can inhibit porcine MYH7 expression by negatively regulating PGC‐1α‐controlled signalling pathways that can benefit MYH7 expression. The present study confirmed that miR‐27a can serve as a negative determinant of muscle fibre type composition aside from its reported role in affecting proliferation of skeletal muscle cells in pigs. Our findings may provide a potential target for control of type I muscle fibre formation, thus having an implication for possible regulation of muscle fibre phenotype and the associated meat quality of pigs.
    Type of Medium: Online Resource
    ISSN: 0931-2439 , 1439-0396
    URL: Issue
    URL: Article
    DOI: 10.1111/jpn.v107.4
    DOI: 10.1111/jpn.13822
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020405-X
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    SSG: 21
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  • 3
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    A Potential Adhesin/Invasin STM0306 Participates in Host Cell Inflammation Induced by Salmonella enterica Serovar Typhimurium
    MDPI AG ; 2023
    In:  International Journal of Molecular Sciences Vol. 24, No. 9 ( 2023-05-03), p. 8170-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 24, No. 9 ( 2023-05-03), p. 8170-
    Abstract: Salmonella enterica serovar typhimurium (S. Typhimurium) is a common Gram-negative foodborne pathogenic bacterium that causes gastrointestinal disease in humans and animals. It is well known that adhesins and invasins play crucial roles in the infection mechanism of S. Typhimurium. S. Typhimurium STM0306 has been denoted as a putative protein and its functions have rarely been reported. In this study, we constructed the STM0306 gene mutant strain of S. Typhimurium and purified the recombinant STM0306 from Escherichia coli. Deletion of the STM0306 gene resulted in reduced adhesion and invasion of S. Typhimurium to IPEC-J2, Caco-2, and RAW264.7 cells. In addition, STM0306 could bind to intestinal epithelial cells and induced F-actin modulation in IPEC-J2 cells. Furthermore, we found that STM0306 activated the nuclear factor kappa B (NF-κB) signaling pathway and increased the mRNA expression of pro-inflammatory cytokines such as IL-1β, TNF-α, as well as chemokine CXCL2, thus resulting in cellular inflammation in host cells. In vivo, the deletion of the STM0306 gene led to reduced pathogenicity of S. Typhimurium, as evidenced by lower fecal bacterial counts and reduced body weight loss in S. Typhimurium infected mice. In conclusion, the STM0306 of S. Typhimurium is an important adhesin/invasin involved in the pathogenic process and cellular inflammation of the host.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms24098170
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2019364-6
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  • 4
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    Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens
    MDPI AG ; 2022
    In:  Toxins Vol. 14, No. 12 ( 2022-11-28), p. 830-
    Details
    In: Toxins, MDPI AG, Vol. 14, No. 12 ( 2022-11-28), p. 830-
    Abstract: Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental CAT on antioxidant property and gut microbiota in DON-exposed broilers. A total of 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups (six replicates/group): control, DON group, and DON + CAT (DONC) group. The control and DON group received a diet without and with DON contamination, respectively, while the DONC group received a DON-contaminated diet with 200 U/kg CAT added. Parameter analysis was performed on d 21. The results showed that DON-induced liver enlargement (p 〈 0.05) was blocked by CAT addition, which also normalized the increases (p 〈 0.05) in hepatic oxidative metabolites contents and caspase-9 expression. Additionally, CAT addition increased (p 〈 0.05) the jejunal CAT and GSH-Px activities coupled with T-AOC in DON-exposed broilers, as well as the normalized DON-induced reductions (p 〈 0.05) of jejunal villus height (VH) and its ratio for crypt depth. There was a difference (p 〈 0.05) in gut microbiota among groups. The DON group was enriched (p 〈 0.05) with some harmful bacteria (e.g., Proteobacteria, Gammaproteobacteria, Enterobacteriales, Enterobacteriaceae, and Escherichia/Shigella) that elicited negative correlations (p 〈 0.05) with jejunal CAT activity, and VH. DONC group was differentially enriched (p 〈 0.05) with certain beneficial bacteria (e.g., Acidobacteriota, Anaerofustis, and Anaerotruncus) that could benefit intestinal antioxidation and morphology. In conclusion, supplemental CAT alleviates DON-induced oxidative stress and intestinal damage in broilers, which can be associated with its ability to improve gut microbiota, aside from its direct oxygen radical-scavenging activity.
    Type of Medium: Online Resource
    ISSN: 2072-6651
    URL: Article
    DOI: 10.3390/toxins14120830
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518395-3
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  • 5
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    Data_Sheet_1.docx
    Frontiers Media SA ; 2022
    In:  Frontiers in Microbiology Vol. 13 ( 2022-10-3)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-10-3)
    Abstract: Glucose oxidase (GOD) could benefit intestinal health and growth performance in animals. However, it is unknown whether GOD can protect piglets against bacterial challenge. This study aimed to evaluate the protective effects of GOD on growth performance, clinical symptoms, serum parameters, and intestinal health in piglets challenged by enterotoxigenic Escherichia coli (ETEC). A total of 44 male weaned piglets around 38 days old were divided into four groups (11 replicates/group): negative control (NC), positive control (PC), CS group (PC piglets +40 g/t colistin sulfate), and GOD group (PC piglets +200 g/t GOD). All piglets except those in NC were challenged with ETEC ( E. coli K88) on the 11th day of the experiment. Parameter analysis was performed on the 21st day of the experiment. The results showed that the ETEC challenge elevated ( p   & lt; 0.05) the rectal temperature and fecal score of piglets at certain time-points post-challenge, reduced ( p   & lt; 0.05) serum glucose and IgG levels but increased ( p   & lt; 0.05) serum alanine aminotransferase activity, as well as caused ( p   & lt; 0.05) intestinal morphology impairment and inflammation. Supplemental GOD could replace CS to reverse ( p   & lt; 0.05) the above changes and tended to increase ( p  = 0.099) average daily gain during the ETEC challenge. Besides, GOD addition reversed ETEC-induced losses ( p   & lt; 0.05) in several beneficial bacteria (e.g., Lactobacillus salivarius ) along with increases ( p   & lt; 0.05) in certain harmful bacteria (e.g., Enterobacteriaceae and Escherichia / Shigella ). Functional prediction of gut microbiota revealed that ETEC-induced upregulations ( p   & lt; 0.05) of certain pathogenicity-related pathways (e.g., bacterial invasion of epithelial cells and shigellosis) were blocked by GOD addition, which also normalized the observed downregulations ( p   & lt; 0.05) of bacterial pathways related to the metabolism of sugars, functional amino acids, nucleobases, and bile acids in challenged piglets. Collectively, GOD could be used as a potential antibiotic alternative to improve growth and serum parameters, as well as attenuate clinical symptoms and intestinal disruption in ETEC-challenged piglets, which could be associated with its ability to mitigate gut microbiota dysbiosis. Our findings provided evidence for the usage of GOD as an approach to restrict ETEC infection in pigs.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2022.994151
    DOI: 10.3389/fmicb.2022.994151.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2587354-4
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  • 6
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    Suitable condition of enzymatic reactivity for determining the digestibility of feedstuffs phosphorus in vitro by dialysis tube method
    Springer Science and Business Media LLC ; 2008
    In:  Frontiers of Agriculture in China Vol. 2, No. 4 ( 2008-12), p. 480-483
    Details
    In: Frontiers of Agriculture in China, Springer Science and Business Media LLC, Vol. 2, No. 4 ( 2008-12), p. 480-483
    Type of Medium: Online Resource
    ISSN: 1673-7334 , 1673-744X
    URL: Article
    DOI: 10.1007/s11703-008-0082-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2008
    SSG: 21
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  • 7
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    Table_1.docx
    Frontiers Media SA ; 2021
    In:  Frontiers in Cellular and Infection Microbiology Vol. 11 ( 2021-11-16)
    Details
    In: Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 11 ( 2021-11-16)
    Abstract: Salmonella Typhimurium is a common pathogen infecting the gastrointestinal tract of humans and animals, causing host gastroenteritis and typhoid fever. Heat shock protein (HtpG) as a molecular chaperone is involved in the various cellular processes of bacteria, especially under environmental stress. However, the potential association of HtpG with S. Typhimurium infection remains unknown. In this study, we clarified that HtpG could also play a role as an effector in S. Typhimurium infection. RNA-seq indicated that the flagellar assembly pathway, infection pathway, and chemotaxis pathway genes of S. Typhimurium were downregulated after the mutation of HtpG, which resulted in compromises of S. Typhimurium motility, biofilm formation, adhesion, invasion, and inflammation-inducing ability. In addition, HtpG recombinant protein was capable of promoting the proliferation of S. Typhimurium in host cells and the resultant inflammation. Collectively, our results illustrated an important role of HtpG in S. Typhimurium infection.
    Type of Medium: Online Resource
    ISSN: 2235-2988
    URL: Article
    URL: Article
    DOI: 10.3389/fcimb.2021.758898
    DOI: 10.3389/fcimb.2021.758898.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2619676-1
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  • 8
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    N-acyl homoserine lactonase attenuates the virulence of Salmonella typhimurium and its induction of intestinal damages in broilers
    Elsevier BV ; 2023
    In:  Animal Nutrition Vol. 14 ( 2023-09), p. 334-342
    Details
    In: Animal Nutrition, Elsevier BV, Vol. 14 ( 2023-09), p. 334-342
    Type of Medium: Online Resource
    ISSN: 2405-6545
    URL: Article
    DOI: 10.1016/j.aninu.2023.04.010
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2834745-6
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  • 9
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    Guar gum-derived galactomannan induces inflammatory responses and increased energy expenditure in the intestine
    Royal Society of Chemistry (RSC) ; 2021
    In:  Food & Function Vol. 12, No. 16 ( 2021), p. 7480-7489
    Details
    In: Food & Function, Royal Society of Chemistry (RSC), Vol. 12, No. 16 ( 2021), p. 7480-7489
    Abstract: Guar gum-derived galactomannan (GGGM) has been widely used in the food industry for a long time and its adverse impacts have been scarcely reported. Galactomannan is considered to have a structure similar to the surface components of certain pathogens, and the present study was thus conducted to investigate if oral administration of GGGM could cause physiological effects that were hypothesized to be related to intestinal inflammatory responses. The results showed that oral administration of GGGM resulted in compromises on growth performance, an increase of the relative weight of spleen and epididymal fat, and an elevation of the α1-acid glycoprotein content in both serum and livers of mice. With regard to energy metabolism-related indices, the activities of intestinal lactic dehydrogenase and succinic dehydrogenase were all increased by the GGGM treatment in both in vivo and in vitro experiments, the latter of which also showed an elevation in the consumption of reducing sugar by intestinal epithelial cells along with a reduced viability of these cells in response to the GGGM treatment. Notably, the GGGM treatment triggered intestinal inflammatory responses that were evidenced by the increased expression of intestinal inflammatory cytokines such as TNF-α and IL-6 both in vivo and in vitro , which were at least partially responsible for the increased energy expenditure in the intestine and the retardation of growth. The results of this study could expand our knowledge of GGGM administration and provide integrated insights into the consumption of GGGM-containing foods.
    Type of Medium: Online Resource
    ISSN: 2042-6496 , 2042-650X
    URL: Article
    DOI: 10.1039/D1FO01143J
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2021
    detail.hit.zdb_id: 2578152-2
    SSG: 21
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  • 10
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    Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Animal Science and Biotechnology Vol. 14, No. 1 ( 2023-01-09)
    Details
    In: Journal of Animal Science and Biotechnology, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2023-01-09)
    Abstract: Salmonella Typhimurium challenge causes a huge detriment to chicken production. N-acyl homoserine lactonase (AHLase), a quorum quenching enzyme, potentially inhibits the growth and virulence of Gram-negative bacteria. However, it is unknown whether AHLase can protect chickens against S. Typhimurium challenge. This study aimed to evaluate the effects of AHLase on growth performance and intestinal health in broilers challenged by S. Typhimurium. A total of 240 one-day-old female crossbred broilers (817C) were randomly divided into 5 groups (6 replicates/group): negative control (NC), positive control (PC), and PC group supplemented with 5, 10 or 20 U/g AHLase. All birds except those in NC were challenged with S. Typhimurium from 7 to 9 days of age. All parameters related to growth and intestinal health were determined on d 10 and 14. Results The reductions ( P 〈 0.05) in body weight (BW) and average daily gain (ADG) in challenged birds were alleviated by AHLase addition especially at 10 U/g. Thus, samples from NC, PC and PC plus 10 U/g AHLase group were selected for further analysis. S. Typhimurium challenge impaired ( P 〈 0.05) intestinal morphology, elevated ( P 〈 0.05) ileal inflammatory cytokines ( IL-1β and IL-8 ) expression, and increased ( P 〈 0.05) serum diamine oxidase (DAO) activity on d 10. However, AHLase addition normalized these changes. Gut microbiota analysis on d 10 showed that AHLase reversed the reductions ( P 〈 0.05) in several beneficial bacteria (e.g. Bacilli, Bacillales and Lactobacillales), along with increases ( P 〈 0.05) in certain harmful bacteria (e.g. Proteobacteria, Gammaproteobacteria, Enterobacteriaceae and Escherichia / Shigella ) in PC group. Furthermore, AHLase-induced increased beneficial bacteria and decreased harmful bacteria were basically negatively correlated ( P 〈 0.05) with the reductions of ileal IL-1β and IL-8 expression and serum DAO activity, but positively correlated ( P 〈 0.05) with the increased BW and ADG. Functional prediction revealed that AHLase abolished S. Typhimurium - induced upregulations ( P 〈 0.05) of certain pathogenicity-related pathways such as lipopolysaccharide biosynthesis, shigellosis, bacterial invasion of epithelial cells and pathogenic Escherichia coli infection of gut microbiota. Conclusions Supplemental AHLase attenuated S. Typhimurium-induced growth retardation and intestinal disruption in broilers, which could be associated with the observed recovery of gut microbiota dysbiosis.
    Type of Medium: Online Resource
    ISSN: 2049-1891
    URL: Article
    DOI: 10.1186/s40104-022-00801-4
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2630162-3
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