In:
Bioscience, Biotechnology, and Biochemistry, Informa UK Limited, Vol. 83, No. 9 ( 2019-09-02), p. 1683-1696
Kurzfassung:
The fully synthetic humanized phage antibody library has the advantages including the minimized immunogenicity, which frequently happened in hybridoma cell-based antibody production. In this paper, using the constructed diverse complementarity determining region gene library and the germline gene as the backbone, we constructed eight single-chain antibody libraries and a combinatorial antibody library with a big capacity of 1.41 × 1010. M13EEA helper phage that was engineered from M13KO7 was applied to prepare phage antibody library. The eukaryotic expression of T-cell immune receptor with Ig and ITIM domain (TIGIT) antigen was used as a target antigen for screening. The screening of antigen-specific single-chain Fc-fused protein was performed through evaluation of binding affinity based on ELISA analysis. The IgG antibody was prepared with the screened single-chain protein. Finally, the CB3 antibody was screened out which exhibits the highest binding affinity with TIGIT with the Kd value of 8.155 × 10−10 M.
Materialart:
Online-Ressource
ISSN:
0916-8451
,
1347-6947
DOI:
10.1080/09168451.2019.1617107
Sprache:
Englisch
Verlag:
Informa UK Limited
Publikationsdatum:
2019
ZDB Id:
2110940-0
SSG:
12
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