In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 11 ( 2021-1-18)
Abstract:
We compared several identification methods for Aeromonas genus members, including traditional biochemical testing, multiplex-PCR amplification, mass spectrometry identification, whole-genome sequencing, multilocus phylogenetic analysis (MLPA), and rpoD, gyrA , and rpoD - gyrA gene sequencing. Isolates ( n = 62) belonging to the Aeromonas genus, which were came from the bacterial bank in the laboratory, were used to assess the identification accuracy of the different methods. Whole-genome sequencing showed that the Aeromonas spp. isolates comprised A. caviae ( n = 21), A. veronii ( n = 18), A. dhakensis ( n = 8), A. hydrophila ( n = 7), A. jandaei ( n = 5), A. enteropelogenes ( n = 2), and A. media ( n = 1). Using the whole-genome sequencing results as the standard, the consistency of the other methods was compared with them. The results were 46.77% (29/62) for biochemical identification, 83.87% (52/62) for mass spectrometric identification, 67.74% (42/62) for multiplex-PCR, 100% (62/62) for MLPA typing, 72.58% for gyrA , and 59.68% for rpoD and gyrA - rpoD . MLPA was the most consistent, followed by mass spectrometry. Therefore, in the public health laboratory, both MLPA and whole-genome sequencing methods can be used to identify various Aeromonas species. However, rapid and relatively accurate mass spectrometry is recommended for clinical lab.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2020.625961
DOI:
10.3389/fmicb.2020.625961.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2587354-4
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