In:
Artificial Organs, Wiley, Vol. 40, No. 6 ( 2016-06), p. 596-603
Kurzfassung:
Hypothermic machine perfusion ( MP ) can reduce graft's injury after kidney transplantation; however, the mechanism has not been elucidated. In the past decade, many studies showed that aldehyde dehydrogenase 2 ( ALDH2 ) is a protease which can inhibit cell apoptosis. Therefore, this study aims to explore whether ALDH2 takes part in reducing organ damage after MP . Eighteen healthy male N ew Z ealand rabbits (12 weeks old, weight 3.0 ± 0.3 kg) were randomly divided into three groups: normal group, MP group, and cold storage ( CS ) group ( n = 6). The left kidney of rabbits underwent warm ischemia for 35 min through clamping the left renal pedicle and then reperfusion for 1 h. Left kidneys were preserved by MP or CS (4°C for 4 h) in vivo followed by the right nephrectomy and 24‐h reperfusion, and then the specimens and blood were collected. Finally, concentration of urine creatinine ( C r), blood urea nitrogen ( BUN ), and 4‐ HNE were tested. Renal apoptosis was detected by TUNEL staining, and the expression of ALDH2, cleaved‐caspase 3, bcl‐2/ bax, MAPK in renal tissue was detected by immunohistochemistry or Western blot; 24 h after surgery, the concentration of C r in MP group was 355 ± 71μmol/L, in CS group was 511 ± 44 μmol/L ( P 〈 0.05), while the BUN was 15.02 ± 2.34 mmol/L in MP group, 22.64 ± 3.58 mmol/L in CS group ( P 〈 0.05). The rate of apoptosis and expression of cleaved caspase‐3, p‐P38, p‐ ERK , and p‐ JNK in MP group was significantly lower than that in CS group ( P 〈 0.05), while expression of ALDH2 and bcl‐2/bax in MP group was significantly higher than that in CS group ( P 〈 0.05); expression of cleaved caspase‐3 in both MP and CS group significantly increased as compared with that in normal group ( P 〈 0.05). In conclusion, increased expression of ALDH2 can reduce the renal cell apoptosis through inhibiting MAPK pathway during ischemia/reperfusion injury ( IRI ) after hypothermic MP.
Materialart:
Online-Ressource
ISSN:
0160-564X
,
1525-1594
DOI:
10.1111/aor.2016.40.issue-6
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2016
ZDB Id:
2003825-2
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