In:
Luminescence, Wiley, Vol. 35, No. 1 ( 2020-02), p. 43-51
Abstract:
A dual‐signal strategy is proposed based on fluorescent biomass‐based carbon dots (BC‐dots) and chitosan stabilized AuNPs (CS@AuNPs) to determine hyaluronidase (HAase). BC‐dots can induce aggregation of CS@AuNPs nanoparticles with a colour change from red to blue. Positively charged CS@AuNPs interacted with the negatively charged hyaluronic acid (HA) through electrostatic adsorption, and CS@AuNPs maintained stability due to the semirigid coil conformation of HA. However, in the presence of HAase, due to enzymatic hydrolysis of HA by HAase, the CS@AuNPs agglomerated. Based on the change of fluorescence and colour, quantitative analysis of HAase was achieved. Linear ranges for the fluorometric and colorimetric determinations were 2.0–70 U mL −1 and 8–60 U mL −1 , respectively, with a detection limit of 0.27 U mL −1 . This dual‐signal sensing system possesses high potential for determination of HAase in biological matrices.
Type of Medium:
Online Resource
ISSN:
1522-7235
,
1522-7243
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2001819-8
SSG:
12
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