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  • SAGE Publications  (7)
  • Zhang, Y.  (7)
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  • SAGE Publications  (7)
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  • 1
    Online Resource
    Online Resource
    SAGE Publications ; 2018
    In:  Journal of Dental Research Vol. 97, No. 3 ( 2018-03), p. 289-295
    In: Journal of Dental Research, SAGE Publications, Vol. 97, No. 3 ( 2018-03), p. 289-295
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2018
    detail.hit.zdb_id: 2057074-0
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  • 2
    Online Resource
    Online Resource
    SAGE Publications ; 2023
    In:  Journal of Dental Research Vol. 102, No. 5 ( 2023-05), p. 536-545
    In: Journal of Dental Research, SAGE Publications, Vol. 102, No. 5 ( 2023-05), p. 536-545
    Abstract: Radiotherapy of head and neck cancers frequently leads to irreversible hypofunction of salivary glands, which severely compromises the quality of life and is extremely difficult to treat. We found recently that salivary gland resident macrophages are sensitive to radiation and interact with epithelial progenitors and endothelial cells through homeostatic paracrine factors. Heterogeneous subpopulations of resident macrophages are present in other organs with distinct functions, whereas subpopulations of salivary gland resident macrophages with distinct functions or transcriptional profiles have not been reported yet. Using single-cell RNA sequencing, we found that mouse submandibular glands (SMGs) contain 2 distinct self-renewing resident macrophage subsets, an MHC-II hi subset present in many other organs and an uncommon Csf2r + subset. The main source of Csf2 in SMGs are innate lymphoid cells (ILCs) that rely on IL15 for maintenance, while the main source of IL15 protein is Csf2r + resident macrophages, indicating a homeostatic paracrine interaction between these cells. Csf2r + resident macrophages are the major source of hepatocyte growth factor (Hgf) that regulates homeostasis of SMG epithelial progenitors. Meanwhile, Csf2r + resident macrophages are responsive to Hedgehog signaling that can rescue salivary function impaired by radiation. Consistently, irradiation persistently decreased numbers of ILCs and levels of IL15 and Csf2 in SMGs, which were all recovered by transient activation of Hedgehog signaling after radiation. Csf2r + resident macrophages and MHC-II hi resident macrophages share transcriptome profiles of perivascular macrophages and macrophages associated with nerves and/or epithelial cells in other organs, respectively, and such niche preferences were supported by lineage tracing and immunofluorescent staining. These findings reveal an uncommon resident macrophage subset that regulates the homeostasis of the salivary gland and is promising as the target to restore salivary gland function impaired by radiation.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2057074-0
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  • 3
    Online Resource
    Online Resource
    SAGE Publications ; 2011
    In:  Technology in Cancer Research & Treatment Vol. 10, No. 4 ( 2011-08), p. 361-369
    In: Technology in Cancer Research & Treatment, SAGE Publications, Vol. 10, No. 4 ( 2011-08), p. 361-369
    Abstract: Angiopoietin-2 (Ang2) has been shown highly expressed in resected human pancreatic carcinoma samples, but the role of it is less clear. We were, therefore, interested in exploring the effects of Ang2 silencing on the angiogenesis and growth of pancreatic carcinoma. Lentivirus mediated Ang2 small hairpin RNA (LV-RNAi) were transfected into pancreatic carcinoma cell line MIA PaCa-2. Three groups were designed in this study: the control group (Mia PaCa-2 cells), the LV-NC group (cells transfected with the control GFP-lentivirus) and the LV-RNAi group (cells transfected with LV-RNAi). The mRNA and protein level of Ang2 gene were detected by real-time polymerase chain reaction and Western blot respectively. MTT assay and Flow Cytometry were used to detect the cell growth and apoptosis. Anti-angiogenesis effect was measured by chick embryo chorioallantoic membrane (CAM) assay. In nude mice bearing tumors, after treatment with intratumoral injection of LV-RNAi, mice growth and tumor volume were observed, and the expression of Ang2, VEGF and CD34 were measured by immunohistochemistry. Compared with the control group and the LV-NC group, the mRNA and protein level of Ang2 gene were successfully knocked down in LV- RNAi group. Also the vessel count was decreased in CAM assay after LV-RNAi transfection. Meanwhile, no obvious cell viability and apoptosis changes were found in MTT assay and Flow Cytometry, respectively. LV-RNAi inhibited pancreatic carcinoma angio- genesis and growth by downregulating the expression of VEGF and CD34. These findings demonstrate that Ang2 gene silencing may exert a anti-angiogenesis effect in vitro and in vivo, and Ang2 targeted gene therapy has the potential to serve as a novel way for pancreatic carcinoma treatment.
    Type of Medium: Online Resource
    ISSN: 1533-0346 , 1533-0338
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
    detail.hit.zdb_id: 2146365-7
    detail.hit.zdb_id: 2220436-2
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  • 4
    Online Resource
    Online Resource
    SAGE Publications ; 2017
    In:  Journal of Dental Research Vol. 96, No. 5 ( 2017-05), p. 539-546
    In: Journal of Dental Research, SAGE Publications, Vol. 96, No. 5 ( 2017-05), p. 539-546
    Abstract: Treatment of bisphosphonate-related osteonecrosis of the jaw (BRONJ) has posed significant challenges to maxillofacial surgeons because of the poor repair of BRONJ bone defects. Moreover, the pathological mechanisms remain unclear. Bone marrow stromal cells (BMSCs) play key roles during bone repair and bone regeneration. However, the activities of BMSCs derived from BRONJ lesions and the BRONJ lesion boundary, as well as the roles of BMSCs in BRONJ defect repair, are poorly defined. In this study, we found that BMSCs from the central area of the osteonecrotic BRONJ region (center-BRONJ BMSCs) and the peripheral area at the recommended debridement boundary (peri-BRONJ BMSCs) had decreased proliferative ability, self-renewal capacity, and multidifferentiation capacities compared with control BMSCs. Osteoclast-inducing ability was also impaired in BRONJ BMSCs. All of these results suggested that the decreased activities of BRONJ BMSCs, even the BMSCs derived from the BRONJ lesion boundary, might be an important factor leading to insufficient bone repair of BRONJ lesions. This study offers early stage evidence for the use of marrow stromal cells in the treatment of BRONJ.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
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  • 5
    Online Resource
    Online Resource
    SAGE Publications ; 2018
    In:  Journal of Dental Research Vol. 97, No. 11 ( 2018-10), p. 1222-1228
    In: Journal of Dental Research, SAGE Publications, Vol. 97, No. 11 ( 2018-10), p. 1222-1228
    Abstract: Ultra-translucent zirconias are drawing immense attention due to their fascinating esthetic appearance. However, the high translucency came at the expense of diminishing strength along with the reduced ability of transformation toughening due to the increased cubic zirconia content. We aim to address these issues by infiltrating glass on the surface of an ultra-translucent zirconia (5Y-PSZ). Glasses of different shades can be used and the resulting graded glass/zirconia layer is expected to improve the material’s flexural strength without compromising its esthetics. We also aim to elucidate how clinically relevant surface treatments—namely, air abrasion, glazing, or polishing—affect the fracture resistance of these zirconias with a high cubic content. All surface treatments were performed on bar-shaped (2 × 3 × 25 mm 3 ) and plate-shaped (12 × 12 × 1 mm 3 ) specimens, which were then subjected to a 4-point bending test and translucency measurements, respectively. 5Y-PSZ proved to be significantly more translucent than 3Y-TZP but also much weaker. Our hypothesis was accepted, as the strength of the glass-infiltrated ultra-translucent 5Y-PSZ (582 ± 20 MPa) is over 70% higher than its uninfiltrated counterpart (324 ± 57 MPa). Its strength is also over 25% higher than the highly polished 5Y-PSZ (467 ± 38 MPa). In addition, the translucency of 5Y-PSZ (translucency parameter [ TP] = 34, contrast ratio [ CR] = 0.31) is not affected by glass infiltration ( TP = 34, CR = 0.32) when the residual surface glass is removed by gentle polishing using 6- and then 3-µm diamond grits. Finally, both air abrasion and the presence of a glaze layer on the tensile surface decreased flexural strength significantly, being 274 ± 55 and 211 ± 21 MPa, respectively. With a combined high strength and translucency, the newly developed glass-infiltrated 5Y-PSZ may be considered a suitable material for next-generation, damage-resistant, and esthetic dental restorations.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2018
    detail.hit.zdb_id: 2057074-0
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  • 6
    Online Resource
    Online Resource
    SAGE Publications ; 2023
    In:  Journal of Dental Research Vol. 102, No. 3 ( 2023-03), p. 302-312
    In: Journal of Dental Research, SAGE Publications, Vol. 102, No. 3 ( 2023-03), p. 302-312
    Abstract: Abnormal stress loading has been considered a major contributor to the initiation of temporomandibular joint osteoarthritis (TMJOA), but studies to date have not identified a functional molecule that transforms physical stress into biological or biochemical signaling in chondrocytes in response to excessive mechanical stress. Ras-related protein Rap-2a (RAP2A) is reportedly a molecular switch that relays extracellular matrix rigidity signals via the Hippo/Yes-associated protein (YAP) pathway. In the present study, RAP2A diminished with cartilage degradation in unilateral anterior crossbite-induced TMJOA mice, as well as severe cartilage matrix degeneration and TMJOA formation in Cre-loxP–mediated conditional RAP2A knockout mice. RAP2A in chondrocytes regulated the Hippo/YAP pathway directly in response to matrix stiffness, and RAP2A/Hippo/YAP was critical for a chondrocyte phenotype switch and matrix synthesis function. Loss of RAP2A impaired cartilage homeostasis and altered chondrocyte phenotype via Hippo/YAP/SRY-box transcription factor 9 signaling. It may be possible to generate therapeutic strategies using RAP2A or YAP to attenuate the TMJOA pathological process at an early stage. This is the first study to reveal the molecular function of RAP2A in TMJOA progression as a mechanotransduction molecule in condylar chondrocytes.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2057074-0
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  • 7
    In: Genes & Cancer, SAGE Publications, Vol. 2, No. 9 ( 2011-09-01), p. 914-922
    Type of Medium: Online Resource
    ISSN: 1947-6019 , 1947-6027
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
    detail.hit.zdb_id: 2538519-7
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