In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-06-22)
Abstract:
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on a large scale efficiently, and to discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish and mice, but it has never been tried in large model animals. We describe a successful systematic three-generation ENU mutagenesis screening in pigs with the establishment of the Chinese Swine Mutagenesis Consortium. A total of 6,770 G1 and 6,800 G3 pigs were screened, 36 dominant and 91 recessive novel pig families with various phenotypes were established. The causative mutations in 10 mutant families were further mapped. As examples, the mutation of SOX10 (R109W) in pig causes inner ear malfunctions and mimics human Mondini dysplasia, and upregulated expression of FBXO32 is associated with congenital splay legs. This study demonstrates the feasibility of artificial random mutagenesis in pigs and opens an avenue for generating a reservoir of mutants for agricultural production and biomedical research.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.26248.001
DOI:
10.7554/eLife.26248.002
DOI:
10.7554/eLife.26248.003
DOI:
10.7554/eLife.26248.004
DOI:
10.7554/eLife.26248.005
DOI:
10.7554/eLife.26248.006
DOI:
10.7554/eLife.26248.007
DOI:
10.7554/eLife.26248.008
DOI:
10.7554/eLife.26248.009
DOI:
10.7554/eLife.26248.010
DOI:
10.7554/eLife.26248.011
DOI:
10.7554/eLife.26248.012
DOI:
10.7554/eLife.26248.013
DOI:
10.7554/eLife.26248.014
DOI:
10.7554/eLife.26248.015
DOI:
10.7554/eLife.26248.016
DOI:
10.7554/eLife.26248.017
DOI:
10.7554/eLife.26248.018
DOI:
10.7554/eLife.26248.019
DOI:
10.7554/eLife.26248.021
DOI:
10.7554/eLife.26248.022
DOI:
10.7554/eLife.26248.020
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
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