In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 5 ( 2021-05-11)
Abstract:
Four Gram-stain-positive, non-motile and asporous bacilli (strains ZJ-599 T , ZJ-621, MC1420 T and MC1482), isolated from animal tissue and environmental samples collected on the Qinghai-Tibet Plateau, PR China, were taxonomically characterized. Based on the results of 16S rRNA gene sequence analyses, the closest relatives of strains ZJ-599 T and ZJ-621 were Corynebacterium endometrii LMM-1653 T (97.5 %), Corynebacterium phocae M408/89/1 T (96.5 %) and Corynebacterium flavescens OJ8 T (96.3 %), whereas strains MC1420 T and MC1482 were closest to Corynebacterium sanguinis CCUG 58655 T (98.9 %), Corynebacterium mycetoides DSM 20632 T (98.4 %) and Corynebacterium lipophiloflavum DSM 44291 T (97.9 %). The results of rpoB gene sequence similarity analysis indicated that C. phocae M408/89/1 T and C. sanguinis CCUG 58655 T were closest to strains ZJ-599 T /ZJ-621 (83.5 %) and MC1420 T /MC1482 (91.8 %), respectively. The two novel type strains shared a similarity of 95.2 % in 16S rRNA and 81.3 % in rpoB gene sequences. The TAP-PCR DNA fingerprint and MALDI-TOF MS spectrum patterns clearly differentiated the novel isolates within and between each pair of strains. Strain ZJ-599 T had 21.9–22.4 % digital DNA–DNA hybridization (dDDH) scores with C. endometrii LMM-1653 T , C. phocae M408/89/1 T and C. flavescens OJ8 T , and 72.3–72.9 % of average nucleotide identity (ANI) with them. Similarly, strain MC1420 T had 22.9–23.7 % dDDH values with C. sanguinis CCUG 58655 T , C. mycetoides DSM 20632 T and C. lipophiloflavum DSM 44291 T , and 80.4–81.3 % ANI scores with them. Strain ZJ-599 T had a 23.1 % dDDH value and 70.5 % ANI score with strain MC1420 T , both below the corresponding thresholds for species delineation. Strains ZJ-599 T and MC1420 T both contain mycolic acids and have MK-8(H 2 ) and MK-9(H 2 ) as the predominant respiratory quinones, meso -diaminopimelic acid as the diagnostic diamino acid, and C 18 : 1 ω 9 c as the main fatty acid. C 17 : 1 ω 8 c and C 15 : 1 ω 8 c were predominant in strain ZJ-599 T in contrast to C 17 : 1 ω 7 c being predominant in strain MC1420 T . The main polar lipids in strain ZJ-599 T were diphosphatidylglycerol, phosphatidylinositol and one unidentified glycolipid, while strain MC1420 T had diphosphatidylglycerol, phosphatidylglycerol and one unidentified lipid as the major components. Since the two pairs of novel strains (ZJ-599 T /ZJ-621, MC1420 T /MC1482) distinctly differ from each other and from their nearest relatives, two novel species of the genus Corynebacterium are proposed, namely Corynebacterium lizhenjunii (type strain ZJ-599 T =GDMCC 1.1779 T =JCM 34341 T ) and Corynebacterium qintianiae (type strain MC1420 T =GDMCC 1.1783 T =JCM 34340 T ), respectively.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004803
Language:
English
Publisher:
Microbiology Society
Publication Date:
2021
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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