In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2019 ( 2019-06-04), p. 1-14
Abstract:
Objective . The aim of this study is to investigate the implication of the Chinese herbal formula (CHF) Shenzhu tiaopi Granule (STG) in type 2 diabetes mellitus (T2DM) and discuss the mechanisms by which STG regulates the gut microbiota. Method . Goto-Kakizaki (GK) rats and age-matched Wistar (W) rats were acclimatized for 1 week. The GK rats were randomly divided into 3 groups and orally gavaged with saline (model group, M), acarbose (acarbose group, A), and STG (granule of CHF group, G; the component of this formula includes Codonopsis pilosula , Rhizoma Atractylodis , Pinellia , Poria cocos , Pericarpium Citri Reticulatae , Coptis chinensis Franch , and Pueraria ). The W rats were orally gavaged with saline (control group, C). The observation time was 8 weeks. The weight, fasting blood glucose (FBG) level, and blood lipid levels were tested. The 16S rRNA genes in the V3-V4 region were sequenced, and the structure of the gut microbiota was analysed. Results . Compared to C, M displayed significant differences in blood glucose, gut microbiota, etc. (P 〈 0.05; P 〈 0.01). Compared to M, A and G showed a similar reduction in the FBG gain and a shift in the structure of the gut microbiota (P 〈 0.05; P 〈 0.01). Compared with A, G exhibited a significant decrease in weight, FBG level, and total cholesterol (P 〈 0.05). The gut microbiota, Bacteroidetes, the Firmicutes/Bacteroidetes ratio, Allobaculum , and Desulfovibrionaceae were significantly decreased in response to the STG treatment, while Lactobacillus was significantly enriched (P 〈 0.05; P 〈 0.01). The community composition also differed at the phylum and genus levels based on the linear discriminant analysis effect size and heat map. Conclusion . Our findings suggest that the composition of the gut microbiota was significantly changed in the diabetic GK rats compared with that in the normal W rats. STG treatment can improve glucose and lipid levels and modulate the gut microbiota in T2DM rats.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/2019/6976394
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2019
detail.hit.zdb_id:
2148302-4
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