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  • Oxford University Press (OUP)  (6)
  • Zhang, Juan  (6)
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  • Oxford University Press (OUP)  (6)
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  • 1
    Online Resource
    Online Resource
    Enhanced thermostability of keratinase by computational design and empirical mutation
    Oxford University Press (OUP) ; 2013
    In:  Journal of Industrial Microbiology and Biotechnology Vol. 40, No. 7 ( 2013-07-01), p. 697-704
    Details
    In: Journal of Industrial Microbiology and Biotechnology, Oxford University Press (OUP), Vol. 40, No. 7 ( 2013-07-01), p. 697-704
    Abstract: Keratinases are proteolytic enzymes capable of degrading insoluble keratins. The importance of these enzymes is being increasingly recognized in fields as diverse as animal feed production, textile processing, detergent formulation, leather manufacture, and medicine. To enhance the thermostability of Bacillus licheniformis BBE11-1 keratinase, the PoPMuSiC algorithm was applied to predict the folding free energy change (ΔΔG) of amino acid substitutions. Use of the algorithm in combination with molecular modification of homologous subtilisin allowed the introduction of four amino acid substitutions (N122Y, N217S, A193P, N160C) into the enzyme by site-directed mutagenesis, and the mutant genes were expressed in Bacillus subtilis WB600. The quadruple mutant displayed synergistic or additive effects with an 8.6-fold increase in the t  1/2 value at 60 °C. The N122Y substitution also led to an approximately 5.6-fold increase in catalytic efficiency compared to that of the wild-type keratinase. These results provide further insight into the thermostability of keratinase and suggest further potential industrial applications.
    Type of Medium: Online Resource
    ISSN: 1476-5535 , 1367-5435
    URL: Article
    DOI: 10.1007/s10295-013-1268-4
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2013
    detail.hit.zdb_id: 1482484-X
    SSG: 12
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11
    Oxford University Press (OUP) ; 2014
    In:  FEMS Microbiology Letters Vol. 355, No. 2 ( 2014-06), p. 170-176
    Details
    In: FEMS Microbiology Letters, Oxford University Press (OUP), Vol. 355, No. 2 ( 2014-06), p. 170-176
    Type of Medium: Online Resource
    ISSN: 0378-1097
    URL: Issue
    URL: Article
    DOI: 10.1111/fml.2014.355.issue-2
    DOI: 10.1111/1574-6968.12457
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2014
    detail.hit.zdb_id: 1501716-3
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Enhancement of glutathione production with a tripeptidase-deficient recombinant Escherichia coli
    Oxford University Press (OUP) ; 2009
    In:  Journal of Industrial Microbiology & Biotechnology Vol. 36, No. 12 ( 2009-12), p. 1447-1452
    Details
    In: Journal of Industrial Microbiology & Biotechnology, Oxford University Press (OUP), Vol. 36, No. 12 ( 2009-12), p. 1447-1452
    Type of Medium: Online Resource
    ISSN: 1367-5435 , 1476-5535
    URL: Article
    DOI: 10.1007/s10295-009-0631-y
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2009
    detail.hit.zdb_id: 1482484-X
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Lactobacillus casei combats acid stress by maintaining cell membrane functionality
    Oxford University Press (OUP) ; 2012
    In:  Journal of Industrial Microbiology and Biotechnology Vol. 39, No. 7 ( 2012-07-01), p. 1031-1039
    Details
    In: Journal of Industrial Microbiology and Biotechnology, Oxford University Press (OUP), Vol. 39, No. 7 ( 2012-07-01), p. 1031-1039
    Abstract: Lactobacillus casei strains have traditionally been recognized as probiotics and frequently used as adjunct culture in fermented dairy products where lactic acid stress is a frequently encountered environmental condition. We have investigated the effect of lactic acid stress on the cell membrane of L. casei Zhang [wild type (WT)] and its acid-resistant mutant Lbz-2. Both strains were grown under glucose-limiting conditions in chemostats; following challenge by low pH, the cell membrane stress responses were investigated. In response to acid stress, cell membrane fluidity decreased and its fatty acid composition changed to reduce the damage caused by lactic acid. Compared with the WT, the acid-resistant mutant exhibited numerous survival advantages, such as higher membrane fluidity, higher proportions of unsaturated fatty acids, and higher mean chain length. In addition, cell integrity analysis showed that the mutant maintained a more intact cellular structure and lower membrane permeability after environmental acidification. These results indicate that alteration in membrane fluidity, fatty acid distribution, and cell integrity are common mechanisms utilized by L. casei to withstand severe acidification and to reduce the deleterious effect of lactic acid on the cell membrane. This detailed comparison of cell membrane responses between the WT and mutant add to our knowledge of the acid stress adaptation and thus enable new strategies to be developed aimed at improving the industrial performance of this species under acid stress.
    Type of Medium: Online Resource
    ISSN: 1476-5535 , 1367-5435
    URL: Article
    DOI: 10.1007/s10295-012-1104-2
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2012
    detail.hit.zdb_id: 1482484-X
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Improved acid-stress tolerance of Lactococcus lactis NZ9000 and Escherichia coli BL21 by overexpression of the anti-acid component recT
    Oxford University Press (OUP) ; 2018
    In:  Journal of Industrial Microbiology and Biotechnology Vol. 45, No. 12 ( 2018-12-01), p. 1091-1101
    Details
    In: Journal of Industrial Microbiology and Biotechnology, Oxford University Press (OUP), Vol. 45, No. 12 ( 2018-12-01), p. 1091-1101
    Abstract: Acid accumulation caused by carbon metabolism severely affects the fermentation performance of microbial cells. Here, different sources of the recT gene involved in homologous recombination were functionally overexpressed in Lactococcus lactis NZ9000 and Escherichia coli BL21, and their acid-stress tolerances were investigated. Our results showed that L. lactis NZ9000 (ERecT and LRecT) strains showed 1.4- and 10.4-fold higher survival rates against lactic acid (pH 4.0), respectively, and that E. coli BL21 (ERecT) showed 16.7- and 9.4-fold higher survival rates than the control strain against lactic acid (pH 3.8) for 40 and 60 min, respectively. Additionally, we found that recT overexpression in L. lactis NZ9000 improved their growth under acid-stress conditions, as well as increased salt- and ethanol-stress tolerance and intracellular ATP concentrations in L. lactis NZ9000. These findings demonstrated the efficacy of recT overexpression for enhancing acid-stress tolerance and provided a promising strategy for insertion of anti-acid components in different hosts.
    Type of Medium: Online Resource
    ISSN: 1476-5535 , 1367-5435
    URL: Article
    DOI: 10.1007/s10295-018-2075-8
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2018
    detail.hit.zdb_id: 1482484-X
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Systemic understanding of Lactococcus lactis response to acid stress using transcriptomics approaches
    Oxford University Press (OUP) ; 2019
    In:  Journal of Industrial Microbiology and Biotechnology Vol. 46, No. 11 ( 2019-11-01), p. 1621-1629
    Details
    In: Journal of Industrial Microbiology and Biotechnology, Oxford University Press (OUP), Vol. 46, No. 11 ( 2019-11-01), p. 1621-1629
    Abstract: During fermentation, acid stress caused by the accumulation of acidic metabolites seriously affects the metabolic activity and production capacity of microbial cells. To elucidate the acid stress-tolerance mechanisms of microbial cells, we performed genome mutagenesis combined with high-throughput technologies to screen acid stress-tolerant strains. Mutant strain Lactococcus lactis WH101 showed a 16,000-fold higher survival rate than that of the parent strain after 5 h of acid shock at pH 4.0 and maintained higher ATP, NH4+, and intracellular pH (pHi) levels during acid stress. Additionally, comparative transcriptomics analysis revealed enhanced regulation of carbohydrate metabolism and sugar transport to provide additional energy, amino acid metabolism and transport to maintain pHi homeostasis and ATP generation, and fatty acid metabolism to enhance cellular acid tolerance. Moreover, overexpression of identified components resulted in 12.6- and 12.9-fold higher survival rates after acid shock for 3 h at pH 4.0 in L. lactis (ArcB) and L. lactis (MalQ) compared to the control strain, respectively. These findings provide valuable insight into the acid stress-response mechanisms of L. lactis and promote the further development of robust industrial strains.
    Type of Medium: Online Resource
    ISSN: 1476-5535 , 1367-5435
    URL: Article
    DOI: 10.1007/s10295-019-02226-x
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2019
    detail.hit.zdb_id: 1482484-X
    SSG: 12
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