In:
BioMed Research International, Hindawi Limited, Vol. 2015 ( 2015), p. 1-10
Abstract:
Objective . To investigate the effects of β -Elemene ( β -ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase II α (TOPO II α ) expression and activity of human hepatocarcinoma HepG-2 cells. Methods . After treatment with β -ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope. Cell proliferation was assessed using an MTT assay, cell cycles were analyzed using flow cytometry, and apoptosis was detected by Annexin V/PI staining. The expression of TOPO I and TOPO II α was analyzed by Western blot techniques, and their activity was measured using the TOPO I-mediated, supercoiled pBR322 DNA relaxation and TOPO II α -mediated Kinetoplast DNA (kDNA) decatenation assays, respectively. Supercoiled pBR322 and kDNA were also used to determine the direct effect of β -ELE on DNA breaks. Results . β -ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. β -ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO II α in a dose-dependent manner. β -ELE also inhibited TOPO I- and TOPO II α -mediated DNA relaxation but did not directly induce DNA breakage at any concentration. Conclusion . β -ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO II α .
Type of Medium:
Online Resource
ISSN:
2314-6133
,
2314-6141
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2015
detail.hit.zdb_id:
2698540-8
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