In:
Autonomic and Autacoid Pharmacology, Wiley, Vol. 23, No. 5-6 ( 2003-10), p. 327-333
Abstract:
1 The aim of this study was to investigate the co‐localization of histamine and dopamine‐ β ‐hydroxylase in the superior cervical ganglion of guinea‐pig and release of histamine from cardiac sympathetic terminals in guinea‐pig isolated atrium. 2 Histidine decarboxylase (a histamine‐synthesizing enzyme) mRNA signals were detected in the neurones of superior cervical ganglion of guinea‐pig by in situ hybridization. The results of double‐labelled immunofluorescence further confirmed the co‐localization of histamine and dopamine‐ β ‐hydroxylase in the large principle neurons and small intensely fluorescent cells in the superior cervical ganglion. The immunoreactivities of both histamine and dopamine‐ β ‐hydroxylase were significantly attenuated after 6‐hydroxydopamine‐induced lesion of sympathetic nerves. 3 The refractory electrical field stimulation caused the release of histamine from cardiac sympathetic terminals of guinea‐pig isolated atria (112.14 ± 40.34 ng ml −1 ), which was significantly attenuated to 35 ± 15.57 ng ml −1 by reserpine pretreatment. Following administering compound 48/80, a mast cell degranulator, electrical field stimulation induced a dramatic increase of endogenous histamine release from isolated atria (303.57 ±72.93 ng ml −1 ). When compound 48/80 was added to the reserpine‐treated atria, the release of histamine induced by field stimulation was decreased to 207.14 ± 76.39 ng ml −1 . 4 These results provide novel evidence that histamine co‐exists with noradrenaline in sympathetic nerves and might act as a neurotransmitter to modulate sympathetic neurotransmission.
Type of Medium:
Online Resource
ISSN:
1474-8665
,
1474-8673
DOI:
10.1111/aap.2003.23.issue-5-6
DOI:
10.1111/j.1474-8673.2004.00305.x
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
2115935-X
SSG:
15,3
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