In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 5 ( 2020-05-01), p. 3361-3373
Abstract:
A novel obligately anaerobic, thermophilic and formate-utilizing bacterium K32 T was isolated from Shengli oilfield of China. Cells were straight rods (0.4–0.8 µm × 2.5–8.0 µm), Gram-stain-positive, non-spore-forming and slightly motile. Optimum growth occurred with pH of 7 and 0.5 g l –1 NaCl under temperature of 55–60 °C. Nitrate could be reduced into nitrite, syntrophic formate oxidation to methane and carbon dioxide occurred when co-culturing strain K32 T and Methanothermobacter thermautotrophicus ΔH. The main cellular fatty acids were iso-C 15 : 0 (24.0 %), anteiso-C 15 : 0 (21.7 %), C 16 : 0 (12.7 %) and C 14 : 0 (10.8 %), and the main polar lipid was phosphatidylglycerol. The G+C content of the genomic DNA was 46.3 mol%. The 16S rRNA gene sequence of K32 T shared ≤90.4 % of sequence similarity to closest type strains of Desulfitibacter alkalitolerans , Calderihabitans maritimus and members of the genus Moorella . Based on the phenotypic, biochemical and genotypic characterization, Zhaonella formicivorans gen. nov., sp. nov. is proposed with K32 T (=CCAM 584 T =DSM 107278 T =CGMCC1.5297 T ) as the type strain, which is the first representative of Zhaonellaceae fam. nov. In addition, the order Thermoanaerobacterales a nd family Peptococcaceae were reclassified, and three novel families in the novel order of Moorellales ord. nov. were also proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004178
Language:
English
Publisher:
Microbiology Society
Publication Date:
2020
detail.hit.zdb_id:
2056611-6
detail.hit.zdb_id:
2002336-4
SSG:
12
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