In:
Journal of Aquatic Animal Health, Wiley, Vol. 26, No. 2 ( 2014-06), p. 59-65
Abstract:
The aim of this study was to analyze the influence of permeability glycoprotein (P‐gp) gene expression on enrofloxacin (ENR) metabolism in aquatic animals. Nile Tilapia Oreochomis niloticus were fed different doses of ENR ranging from 0 to 80 mg/kg. The P‐gp gene expression levels were determined by quantitative real‐time PCR (qRT‐PCR) at indicated time points after drug administration. Drug metabolism was determined by HPLC. The P‐gp gene expression in liver and kidney was greatly enhanced 30 min after ENR administration at 40 mg/kg, peaked 3 h after drug administration, and then gradually decreased. Thirty minutes after a single oral administration of ENR (0, 20, 40, or 80 mg/kg), the P‐gp gene expression increased in a dose‐dependent manner. The P‐gp gene expression levels in the kidney were significantly higher than those in the liver. Additionally, the metabolic rate of ENR in kidney was more rapid than that in liver. Furthermore, a close correlation was found between P‐gp gene expression and ENR concentrations. These results suggest that P‐gp may be involved in the ENR metabolism process in Nile Tilapia, providing a novel model for the potential utility of gene expression and drug metabolism studies in aquatic animals. Received July 9, 2013; accepted October 17, 2013
Type of Medium:
Online Resource
ISSN:
0899-7659
,
1548-8667
DOI:
10.1080/08997659.2013.860059
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2192418-1
SSG:
22
SSG:
21,3
SSG:
12
Permalink
