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  • 1
    Online Resource
    Online Resource
    American Chemical Society (ACS) ; 2022
    In:  Analytical Chemistry Vol. 94, No. 23 ( 2022-06-14), p. 8174-8180
    In: Analytical Chemistry, American Chemical Society (ACS), Vol. 94, No. 23 ( 2022-06-14), p. 8174-8180
    Type of Medium: Online Resource
    ISSN: 0003-2700 , 1520-6882
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2022
    detail.hit.zdb_id: 1483443-1
    detail.hit.zdb_id: 1508-8
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  • 2
    Online Resource
    Online Resource
    IOP Publishing ; 2017
    In:  IOP Conference Series: Materials Science and Engineering Vol. 207 ( 2017-06), p. 012017-
    In: IOP Conference Series: Materials Science and Engineering, IOP Publishing, Vol. 207 ( 2017-06), p. 012017-
    Type of Medium: Online Resource
    ISSN: 1757-8981 , 1757-899X
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2017
    detail.hit.zdb_id: 2506501-4
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  • 3
    Online Resource
    Online Resource
    MDPI AG ; 2023
    In:  Remote Sensing Vol. 15, No. 22 ( 2023-11-16), p. 5384-
    In: Remote Sensing, MDPI AG, Vol. 15, No. 22 ( 2023-11-16), p. 5384-
    Abstract: Data-driven flood susceptibility modeling is an efficient way to map the spatial distribution of flood likelihood. The quality of the flood susceptibility model relies on the learning technique and the data used for learning. The performance of learning techniques has been extensively examined. However, to date, the impact of data sampling strategies has received limited attention. Random sampling is widely favored because of its ease of use. It treats flood-related data as tabular and excludes their spatial dimensions. Flood occurrence is typically uneven over space. Therefore, non-flood sampling should not be completely random. To represent the impact of the spatial dimension, this study proposed a new sampling approach based on spatial dependence, called inverse-occurrence sampling. It selects more non-flood data in low-risk areas than in high-risk areas. The new sampling approach was compared with random and stratified sampling, using six machine learning techniques in two urban areas in Guangzhou, China, with distinct flood mechanisms, that is, Tianhe (flood density 1.5/km2, clustered distribution, average slope 9.02°, downtown district) and Panyu (flood density 0.15/km2, random distribution, average slope 4.55°, suburban district). Learning techniques include support vector machine (SVM), random forest (RF), artificial neural networks (ANNs), convolutional neural networks (CNNs), CNN-SVM, and CNN-RF. The main findings of this study were as follows: (1) Sampling approaches had a greater impact on model performance than learning techniques in terms of area under the receiver operating characteristic curve (AUC). The AUC variations caused by learning techniques ranged from 0.04 to 0.09. Meanwhile, the AUC variations caused by sampling approaches were between 0.15 and 0.22, all larger than 0.1. (2) The new sampling approach outperformed that of the other two sampling approaches for high average AUC values and small AUC variations. The outperformance is robust in regard to multiple learning techniques and different flooding mechanisms. AUCs in the inverse group had a narrower range (0.14–0.18 in Tianhe and 0.35–0.39 in Panyu) than in the random group (0.24–0.28 in Tianhe and 0.43–0.53 in Panyu) and the stratified group (0.23–0.30 in Tianhe and 0.42–0.48 in Panyu). (3) The most accurate learning technique for AUC was CNN-RF, followed by SVM, CNN-SVM, RF, CNN, and ANN. (4) ANN- and CNN-based models tended to produce polarized patterns in flood susceptibility maps, contradicting the ascending order of flood density with increasing susceptibility levels. Flood density outliers tended to appear in the models derived using RF and CNN-RF. Finally, the newly proposed sampling approach is suggested to be applied to flood susceptibility mapping to reflect the impact of spatial dependence.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2513863-7
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  • 4
    In: Chemical Science, Royal Society of Chemistry (RSC), Vol. 14, No. 15 ( 2023), p. 4102-4113
    Abstract: Orthogonal therapy that combines CRISPR-based gene editing and prodrug-based chemotherapy is a promising approach to combat multidrug-resistant cancer. However, its potency to precisely regulate different therapeutic modalities in vivo is limited due to the lack of an integrated platform with high spatiotemporal resolution. Taking advantage of CRISPR technology, a Pt( iv )-based prodrug and orthogonal emissive upconversion nanoparticles (UCNPs), we herein rationally designed the first logic-gated CRISPR-Cas13d-based nanoprodrug for orthogonal photomodulation of gene editing and prodrug release for enhanced cancer therapy. The nanoprodrug (URL) was constructed by encapsulating a green light-activatable Pt( iv ) prodrug and UV light-activatable Cas13d gene editing tool into UCNPs. We demonstrated that URL maintained excellent orthogonal emission behaviors under 808 and 980 nm excitations, allowing wavelength-selective photoactivation of Cas13d and the prodrug for downregulation of the resistance-related gene and induction of chemo-photodynamic therapy, respectively. Moreover, the photomodulation superiority of URL for overcoming drug resistance was highlighted by integrating it with a Boolean logic gate for programmable modulation of multiple cell behaviors. Importantly, in vivo studies demonstrated that URL can promote Pt( iv ) prodrug activation and ROS generation and massively induce on-target drug accumulation by Cas13d-mediated drug resistance attenuation, delivering an ultimate chemo-photodynamic therapeutic performance in efficiently eradicating primary tumors and preventing further liver metastasis. Collectively, our results suggest that URL expands the Cas13d-based genome editing toolbox into prodrug nanomedicine and accelerates the discovery of new orthogonal therapeutic approaches.
    Type of Medium: Online Resource
    ISSN: 2041-6520 , 2041-6539
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2023
    detail.hit.zdb_id: 2559110-1
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  • 5
    Online Resource
    Online Resource
    American Chemical Society (ACS) ; 2022
    In:  Analytical Chemistry Vol. 94, No. 48 ( 2022-12-06), p. 16622-16631
    In: Analytical Chemistry, American Chemical Society (ACS), Vol. 94, No. 48 ( 2022-12-06), p. 16622-16631
    Type of Medium: Online Resource
    ISSN: 0003-2700 , 1520-6882
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2022
    detail.hit.zdb_id: 1483443-1
    detail.hit.zdb_id: 1508-8
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  • 6
    In: Advanced Therapeutics, Wiley
    Abstract: The cGAS‐STING (cyclic GMP‐AMP synthase/stimulator of interferon genes) pathway is emerging as a promising target for cancer immunotherapy. However, developing specific and effective strategies for activating the cGAS‐STING pathway in tumors is still challenging. Here, a microRNA‐21 (miR‐21)‐responsive nucleic acid system, as a STING signal amplifier, is designed based on the branched catalytic hairpin assembly (bCHA). The effects of three types of dsDNA structures (linear dsDNA, Y scaffold dsDNA, and dsDNA dendrimer) on cGAS‐STING activation are systematically studied. This study demonstrates that dsDNA dendrimer can induce the most effective liquid–liquid phase separation in a miR‐21‐dependent manner, allowing the controllable activation of cGAS‐STING in both cancer cells and dendritic cells. Given the programmable nature of nucleic acid structure, this study will enable a readily accessible platform for integrating with immunotherapeutic strategies while opening new avenues for controllable, tumor‐specific activation of STING agonists.
    Type of Medium: Online Resource
    ISSN: 2366-3987 , 2366-3987
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2920320-X
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  • 7
    Online Resource
    Online Resource
    Elsevier BV ; 2024
    In:  Sensors and Actuators B: Chemical Vol. 410 ( 2024-07), p. 135656-
    In: Sensors and Actuators B: Chemical, Elsevier BV, Vol. 410 ( 2024-07), p. 135656-
    Type of Medium: Online Resource
    ISSN: 0925-4005
    RVK:
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 1500731-5
    detail.hit.zdb_id: 1021505-0
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