In:
International Journal of Rheumatic Diseases, Wiley, Vol. 19, No. 3 ( 2016-03), p. 226-232
Abstract:
The purposes of this study were to investigate senescence‐associated beta‐galactosidase ( SA ‐beta‐ G al) levels in articular cartilage of knee osteoarthritis ( OA ) and the relationship with severity of the disease. Methods All the 50 cartilage tissues, including normal (controls) and OA cartilage were ascribed to four groups of normal, mild lesions, moderate lesions and severe lesions on the basis of the modified M ankin score. Immunohistochemistry was used to assess the SA ‐beta‐ G al expression in articular cartilage. Results No SA ‐beta‐ G al staining was observed in the normal articular cartilage samples. SA ‐beta‐ G al staining was found in a subset of the chondrocytes close to the lesion site of mild, moderate and severe damaged knee OA cartilage. The percentage of SA ‐beta‐ G al‐positive chondrocytes in articular cartilage was 0% in controls, 13.00 ± 5.77% in mild lesions, 31.65 ± 6.91% in moderate lesions and 51.95 ± 6.21% in severe lesions. SA ‐beta‐ G al expression in mild lesions, moderate lesions and severe lesions was higher compared with that of controls ( P 〈 0.0001). SA ‐beta‐ G al expression in moderate lesions and severe lesions were higher with respect to mild lesion samples ( P 〈 0.0001). SA ‐beta‐ G al expression in severe lesions was elevated compared with those of moderate lesions ( P 〈 0.0001). Subsequent analysis showed that articular cartilage SA ‐beta‐ G al levels correlated with severity of disease ( S pearman's ρ = 0.94, P 〈 0.0001). Conclusion SA ‐beta‐ G al expression in articular cartilage is associated with progressive knee OA joint damage and is a potential indictor of disease severity.
Type of Medium:
Online Resource
ISSN:
1756-1841
,
1756-185X
DOI:
10.1111/apl.2016.19.issue-3
DOI:
10.1111/1756-185X.12096
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2427877-4
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