In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 90, No. 2 ( 1989), p. 182-189
Abstract:
Sensitive radioimmunoassays were developed to measure the two groups of major allergens ( 〈 i 〉 Der 〈 /i 〉 I and 〈 i 〉 Der 〈 /i 〉 II) of 〈 i 〉 Dermatophagoides pteronyssinus 〈 /i 〉 and 〈 i 〉 Dermatophagoides farinae 〈 /i 〉 , in which radiolabeled protein A served as a general tracer. Glass rods covalently coupled with F(ab′) 〈 sub 〉 2 〈 /sub 〉 fragments of rabbit antiserum IgG were incubated first with mite or house dust extracts, and then with affinity-purified rabbit antibodies. The bound allergen-antibody complex was detected with a 〈 sup 〉 125 〈 /sup 〉 I-labeled protein A. The two 〈 i 〉 Der 〈 /i 〉 I allergens, 〈 i 〉 Der p 〈 /i 〉 I and 〈 i 〉 Der f 〈 /i 〉 I, were measured separately with the use of immunoabsorbed rabbit antibodies directed against species-specific determinants, while the two 〈 i 〉 Der 〈 /i 〉 II allergens, 〈 i 〉 Der p 〈 /i 〉 II and 〈 i 〉 Der f 〈 /i 〉 II, were measured as ‘ 〈 i 〉 Der 〈 /i 〉 II’ with the use of antibodies directed against common determinants on both allergens. Each assay demonstrated consistently parallel dilution curves with mite and house dust extracts. The mean intraassay and interassay coefficient of variation for each assay ranged from 4.3 to 7.7% and from 3.6 to 9.0%, respectively. The 〈 i 〉 Der p 〈 /i 〉 I and 〈 i 〉 Der f 〈 /i 〉 I assays were shown to be highly species-specific so that the ratio of 〈 i 〉 Der p 〈 /i 〉 l: 〈 i 〉 Der f 〈 /i 〉 I would provide a good index of the distribution of the two mite species in a dust sample. The concentrations of the 〈 i 〉 Der 〈 /i 〉 I and 〈 i 〉 Der 〈 /i 〉 II allergens in different types of mite extract and dust samples from houses were compared using these assays. The results demonstrated that the ratio of 〈 i 〉 Der 〈 /i 〉 I: 〈 i 〉 Der 〈 /i 〉 II in the mite body extracts varied markedly from those in the whole culture extracts, while the ratio in individual dust samples was nearly constant and was close to that in the mite body extracts. These assays will be useful in standardizing mite and house dust extracts and for assessments of mite allergen exposure. Furthermore, the high assay sensitivity will make it possible to use these assays to measure mite allergens associated with airborne particles.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
1989
detail.hit.zdb_id:
1482722-0
Permalink
