In:
Animal Bioscience, Asian Australasian Association of Animal Production Societies, Vol. 34, No. 1 ( 2021-01-01), p. 93-101
Abstract:
Objective: Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood.Methods: A total of 300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium, and low temperature (HT, MT, and LT), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis.Results: Our data showed that compared to the MT group, there was a decrease of muscle leucine and tyrosine (p 〈 0.05), as well as an increase of methionine in the HT group (p 〈 0.05) and a decrease of serine in the LT group. Examination of microbiota shift revealed that at genus level, the relative abundance of 〈 i 〉 Turicibacter 〈 /i 〉 and 〈 i 〉 Parabacteroides 〈 /i 〉 was increased in the HT group (p 〈 0.05) and that the relative abundances of 〈 i 〉 Pandoraea, Achromobacter, Prevotella, Brevundimonas 〈 /i 〉 , and 〈 i 〉 Stenotrophomonas 〈 /i 〉 in the LT group were higher than those in the MT group (p 〈 0.05). In addition, there were substantial correlations between microbes and amino acids. In the HT group. Turicibacter was negatively correlated with aspartic acid and tyrosine, whereas 〈 i 〉 Parabacteroides 〈 /i 〉 was positively correlated with methionine (p 〈 0.05). In the LT group, there were multiple positive correlations between 〈 i 〉 Achromobacter 〈 /i 〉 and arginine, isoleucine or tyrosine; between 〈 i 〉 Prevotella 〈 /i 〉 and cysteine or phenylalanine; between 〈 i 〉 Brevundimonas 〈 /i 〉 and cysteine; and between 〈 i 〉 Stenotrophomonas 〈 /i 〉 and cysteine as well as a negative correlation between 〈 i 〉 Stenotrophomonas 〈 /i 〉 and serine.Conclusion: Our findings demonstrated that amino acid content of breast muscle and intestinal microbiota profile was affected by different ambient temperatures. Under heat exposure, augmented abundance of 〈 i 〉 Parabacteroides 〈 /i 〉 was correlated with elevated methionine. Low temperature treatment may affect muscle tyrosine content through the regulation of 〈 i 〉 Achromobacter 〈 /i 〉 .
Type of Medium:
Online Resource
ISSN:
2765-0189
,
2765-0235
DOI:
10.5713/ajas.20.0314
Language:
English
Publisher:
Asian Australasian Association of Animal Production Societies
Publication Date:
2021
detail.hit.zdb_id:
3055169-9
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