In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2016-04-19)
Abstract:
In the present study, the agonists and antagonists of DP receptor were used to examine whether the PGD 2 -DP signaling pathway affects neuronal function. Primary cultured hippocampal neuron was prepared and treated with aluminum maltolate (100 μM) to establish the neuronal damage model. PGD 2 and cAMP content was detected by ELISA. L-PGDS and DPs mRNA and protein expression were measured by RT-PCR and Western blotting, respectively. The aluminium-load neuron was treated with the DP 1 agonist BW245C, the DP 1 antagonist BWA868C, the DP 2 agonist DK-PGD 2, and the DP 2 antagonist CAY10471, respectively. Neuronal pathomorphology was observed using H-E staining. The cell viability and the lactate dehydrogenase leakage rates of neurons were measured with MTT and LDH kit, respectively. Ca 2+ level was detected by Fluo-3/AM. In the model group, the MTT values obviously decreased; LDH leakage rates and PGD 2 content increased significantly; L-PGDS, DP 1 mRNA and protein expressions increased and DP 2 level decreased. BW245C reduced the Ca 2+ fluorescence intensity and protected the neurons. DK-PGD 2 increased the intensity of Ca 2+ fluorescence, while CAY10471 had the opposite effect. In conclusion, contrary to the effect of DP 2 , the PGD 2 -DP 1 signaling pathway protects against the primary cultured rat hippocampal neuronal injury caused by aluminum overload.
Type of Medium:
Online Resource
ISSN:
2045-2322
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2016
detail.hit.zdb_id:
2615211-3
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