In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 6 ( 2023-6-6), p. e3002131-
Abstract:
Orcinol glucoside (OG), mainly found in the rhizome of the traditional Chinese herb Curculigo orchioides Gaertn, is noted for its antidepressant effects. In this study, an efficient screening pipeline was established for identifying the highly active orcinol synthase (ORS) and UDP-dependent glycosyltransferase (UGT) involved in the biosynthesis of OG by combining transcriptome analysis, structure-based virtual screening, and in vitro enzyme activity assays. By enhancing the downstream pathway, metabolic engineering and fermentation optimization, the OG production in Yarrowia lipolytica was improved 100-fold, resulting in a final yield of 43.46 g/L (0.84 g/g DCW), which is almost 6,400-fold higher than the extraction yield from C . orchioides roots. This study provides a reference for rapid identification of functional genes and high-yield production of natural products.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002131
DOI:
10.1371/journal.pbio.3002131.g001
DOI:
10.1371/journal.pbio.3002131.g002
DOI:
10.1371/journal.pbio.3002131.g003
DOI:
10.1371/journal.pbio.3002131.g004
DOI:
10.1371/journal.pbio.3002131.g005
DOI:
10.1371/journal.pbio.3002131.s001
DOI:
10.1371/journal.pbio.3002131.s002
DOI:
10.1371/journal.pbio.3002131.s003
DOI:
10.1371/journal.pbio.3002131.s004
DOI:
10.1371/journal.pbio.3002131.s005
DOI:
10.1371/journal.pbio.3002131.s006
DOI:
10.1371/journal.pbio.3002131.s007
DOI:
10.1371/journal.pbio.3002131.s008
DOI:
10.1371/journal.pbio.3002131.s009
DOI:
10.1371/journal.pbio.3002131.s010
DOI:
10.1371/journal.pbio.3002131.s011
DOI:
10.1371/journal.pbio.3002131.s012
DOI:
10.1371/journal.pbio.3002131.s013
DOI:
10.1371/journal.pbio.3002131.s014
DOI:
10.1371/journal.pbio.3002131.s015
DOI:
10.1371/journal.pbio.3002131.s016
DOI:
10.1371/journal.pbio.3002131.s017
DOI:
10.1371/journal.pbio.3002131.s018
DOI:
10.1371/journal.pbio.3002131.s019
DOI:
10.1371/journal.pbio.3002131.s020
DOI:
10.1371/journal.pbio.3002131.s021
DOI:
10.1371/journal.pbio.3002131.s022
DOI:
10.1371/journal.pbio.3002131.s023
DOI:
10.1371/journal.pbio.3002131.s024
DOI:
10.1371/journal.pbio.3002131.s025
DOI:
10.1371/journal.pbio.3002131.s026
DOI:
10.1371/journal.pbio.3002131.s027
DOI:
10.1371/journal.pbio.3002131.s028
DOI:
10.1371/journal.pbio.3002131.s029
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
Permalink