In:
BioMed Research International, Hindawi Limited, Vol. 2016 ( 2016), p. 1-7
Abstract:
β -mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the β -mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a “two-step” method comprising ultrafiltration and gel chromatography. The purified β -mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4.5–7.0. The β -mannanase activity was significantly enhanced in the presence of Mn 2+ , Cu 2+ , Zn 2+ , Ca 2+ , Mg 2+ , and Al 3+ and strongly inhibited by Ba 2+ and Pb 2+ . K m and V m a x values for locust bean gum were 7.14 mg/mL and 107.5 μ mol/min/mL versus 1.749 mg/mL and 33.45 µ mol/min/mL for Konjac glucomannan, respectively. Therefore, β -mannanase purified by this work shows stability at high temperatures and in weakly acidic or neutral environments. Based on such data, the β -mannanase will have potential applications as a dietary supplement in treatment of inflammatory processes.
Type of Medium:
Online Resource
ISSN:
2314-6133
,
2314-6141
DOI:
10.1155/2016/6380147
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2016
detail.hit.zdb_id:
2698540-8
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