In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_9 ( 2013-09-01), p. 3274-3279
Abstract:
A Gram-reaction-positive, non-motile, non-spore-forming, catalase-negative, facultatively anaerobic, rod-shaped, β-glucosidase-producing lactic acid bacterium, designated strain THK-V8 T , was isolated from the Korean fermented food, Kimchi, and its taxonomic position was investigated by using a polyphasic approach. Strain THK-V8 T was able to grow at 4–40 °C (optimum, 30 °C) and pH 4.0–7.0 (optimum, pH 6.0). Strain THK-V8 T had the ability to transform ginsenoside Rb 1 to Rd. On the basis of 16S rRNA gene sequence similarity data, strain THK-V8 T was shown to belong to the genus Lactobacillus . Strain THK-V8 T was related to Lactobacillus koreensis DCY50 T (98.8 % sequence similarity), Lactobacillus parabrevis LMG 11984 T (97.7 %), Lactobacillus senmaizukei L13 T (97.5 %), Lactobacillus hammesii TMW1.1236 T (97.3 %) and Lactobacillus brevis ATCC 14687 T (97.2 %). Subsequently, sequence analysis of the RNA polymerase alpha subunit gene ( rpoA ) confirmed that strain THK-V8 T showed a maximum rpoA gene sequence similarity value of 93 % with Lactobacillus brevis LMG 6906 T . The G+C content of the genomic DNA was 47.8 mol%. The DNA–DNA hybridization values between strain THK-V8 T and Lactobacillus parabrevis DCY50 T and Lactobacillus parabrevis LMG 11984 T were 46.1±4.9 % and 10.6±2.9 %, respectively. The major fatty acids were summed feature 7 (comprised of C 19 : 0 cyclo ω10 c /19ω6), C 14 : 0 , C 16 : 0 and C 18 : 1 ω9 c . The cell wall peptidoglycan was of the A4α l -Lys– d -Asp type. The phenotypic and molecular properties indicated that strain THK-V8 T represents a novel species within the genus Lactobacillus , for which the name Lactobacillus yonginensis sp. nov. is proposed. The type strain is THK-V8 T ( = KACC 16236 T = JCM 18023 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.045799-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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