In:
Frontiers in Pharmacology, Frontiers Media SA, Vol. 12 ( 2021-11-16)
Abstract:
Sheng-Ji Hua-Yu (SJHY) formula has been proved to reduce the severity of diabetic wound healing without significant adverse events in our previous clinical trials. However, based on multi-target characteristics, the regulatory network among herbs, ingredients, and hub genes remains to be elucidated. The current study aims to identify the biomarkers of the SJHY formula for the treatment of diabetic wound healing. First, a network of components and targets for the SJHY formula was constructed using network pharmacology. Second, the ClusterONE algorithm was used to build a modular network and identify hub genes along with kernel pathways. Third, we verified the kernel targets by molecular docking to select hub genes. In addition, the biomarkers of the SJHY formula were validated by animal experiments in a diabetic wound healing mice model. The results revealed that the SJHY formula downregulated the mRNA expression of Cxcr4 , Oprd1 , and Htr2a , while upregulated Adrb2 , Drd , Drd4 , and Hrh1 . Besides, the SJHY formula upregulated the kernel pathways, neuroactive ligand–receptor interaction, and cAMP signaling pathway in the skin tissue homogenate of the diabetic wound healing mice model. In summary, this study identified the potential targets and kernel pathways, providing additional evidence for the clinical application of the SJHY formula for the treatment of diabetic wound healing.
Type of Medium:
Online Resource
ISSN:
1663-9812
DOI:
10.3389/fphar.2021.726158
DOI:
10.3389/fphar.2021.726158.s001
DOI:
10.3389/fphar.2021.726158.s002
DOI:
10.3389/fphar.2021.726158.s003
DOI:
10.3389/fphar.2021.726158.s004
DOI:
10.3389/fphar.2021.726158.s005
DOI:
10.3389/fphar.2021.726158.s006
DOI:
10.3389/fphar.2021.726158.s007
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2587355-6
SSG:
15,3
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