In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 11 ( 2020-11-9), p. e0241889-
Abstract:
Raphidocelis subcapitata is one of the most frequently used species for algal growth inhibition tests. Accordingly, many microalgal culture collections worldwide maintain R . subcapitata for distribution to users. All R . subcapitata strains maintained in these collections are derived from the same cultured strain, NIVA-CHL1. However, considering that 61 years have passed since this strain was isolated, we suspected that NIVA-CHL1 in culture collections might have acquired various mutations. In this study, we compared the genome sequences among NIVA-CHL1 from 8 microalgal culture collections and one laboratory in Japan to evaluate the presence of mutations. We found single-nucleotide polymorphisms or indels at 19,576 to 28,212 sites per strain in comparison with the genome sequence of R . subcapitata NIES-35, maintained at the National Institute for Environmental Studies, Tsukuba, Japan. These mutations were detected not only in non-coding but also in coding regions; some of the latter mutations may affect protein function. In growth inhibition test with 3,5-dichlorophenol, EC50 values varied 2.6-fold among the 9 strains. In the ATCC 22662–2 and CCAP 278/4 strains, we also detected a mutation in the gene encoding small-conductance mechanosensitive ion channel, which may lead to protein truncation and loss of function. Growth inhibition test with sodium chloride suggested that osmotic regulation has changed in ATCC 22662–2 and CCAP 278/4 in comparison with NIES-35.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0241889
DOI:
10.1371/journal.pone.0241889.g001
DOI:
10.1371/journal.pone.0241889.g002
DOI:
10.1371/journal.pone.0241889.g003
DOI:
10.1371/journal.pone.0241889.g004
DOI:
10.1371/journal.pone.0241889.g005
DOI:
10.1371/journal.pone.0241889.g006
DOI:
10.1371/journal.pone.0241889.t001
DOI:
10.1371/journal.pone.0241889.t002
DOI:
10.1371/journal.pone.0241889.t003
DOI:
10.1371/journal.pone.0241889.t004
DOI:
10.1371/journal.pone.0241889.s001
DOI:
10.1371/journal.pone.0241889.r001
DOI:
10.1371/journal.pone.0241889.r002
DOI:
10.1371/journal.pone.0241889.r003
DOI:
10.1371/journal.pone.0241889.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2267670-3
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