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  • MDPI AG  (13)
  • Xu, Wentao  (13)
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  • MDPI AG  (13)
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  • 1
    Online Resource
    Online Resource
    Study on Dynamic Monitoring of Wire Rope Tension Based on the Particle Damping Sensor
    MDPI AG ; 2019
    In:  Sensors Vol. 19, No. 2 ( 2019-01-18), p. 388-
    Details
    In: Sensors, MDPI AG, Vol. 19, No. 2 ( 2019-01-18), p. 388-
    Abstract: Real-time monitoring of wire rope tension is of great significance to the safe operation of mine hoist. Due to the longitudinal and lateral coupling vibration of wire ropes during the operation of hoist, there are high frequency components in measured tension signals of wire ropes, which cannot effectively characterize the actual lifting load. To overcome this problem, a particle damping sensor with a vibration dissipation function is designed in this paper. Multilayered steel balls are placed into the cylindrical cavity of the sensor. Damping vibration and energy dissipation will occur when the sensor is subjected to external excitation. Then, to obtain the optimal sensor characteristics, relevant parameters of the particles and the spoke structure are simulated. Finally, the sensor based on the optimized parameters is manufactured and tested in a coal mine. Compared with the general pressure sensor, the particle damping sensor can effectively eliminate the influence of wire ropes vibration on tension measurement and achieve accurate measurement results.
    Type of Medium: Online Resource
    ISSN: 1424-8220
    URL: Article
    DOI: 10.3390/s19020388
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2052857-7
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  • 2
    Online Resource
    Online Resource
    Comprehensive Analysis of the Characteristics and Differences in Adult and Newborn Brown Adipose Tissue (BAT): Newborn BAT Is a More Active/Dynamic BAT
    MDPI AG ; 2020
    In:  Cells Vol. 9, No. 1 ( 2020-01-14), p. 201-
    Details
    In: Cells, MDPI AG, Vol. 9, No. 1 ( 2020-01-14), p. 201-
    Abstract: Brown adipose tissue (BAT) plays an essential role in maintaining body temperature and in treating obesity and diabetes. The adult BAT (aBAT) and neonatal BAT (neBAT) vary greatly in capacity, but the characteristics and differences between them on the molecular level, as well as the related features of BAT as it develops post-delivery, have not yet been fully determined. In this study, we examined the morphological features of aBAT and neBAT of mice by using hematoxylin-eosin (H & E) staining, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). We found that neBAT contains a smaller number and size of lipid droplets, as well as more abundant mitochondria, compared with aBAT. The dynamic morphological changes revealed that the number and size of lipid droplets increase, but the number of mitochondria gradually decrease during the post-delivery development, which consisted of some differences in RNA or protein expression levels, such as gradually decreased uncoupling protein 1 (UCP1) expression levels and mitochondrial genes, such as mitochondrial transcription factor A (Tfam). The adipocyte differentiation-related genes, such as transcription factor CCAAT enhancer-binding protein β (CEBPβ), were also continuously upregulated. Additionally, the different features of aBAT and neBAT were analyzed from the global transcription (RNA-Seq) level, which included messenger RNA (mRNA), microRNA, long non-coding RNA (lncRNA), circRNA, and DNA methylation, as well as proteins (proteomics). Differentially methylated region (DMR) analysis identified 383 hyper- and 503 hypo-methylated genes, as well as 1221 new circRNA in ne-BAT and 1991 new circRNA in a-BAT, with significantly higher expression of circRNA in aBAT compared with neBAT. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that mitochondrial activity, protein synthesis, and cell life activity levels were higher in neBAT, and pathways related to ribosomes, spliceosomes, and metabolism were significantly activated in neBAT compared to aBAT. Collectively, this study describes the dynamic changes occurring throughout post-delivery development from the morphological, molecular and omics perspectives. Our study provides information that may be utilized in improving BAT functional activity through gene regulation and/or epigenetic regulation.
    Type of Medium: Online Resource
    ISSN: 2073-4409
    URL: Article
    DOI: 10.3390/cells9010201
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2661518-6
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  • 3
    Online Resource
    Online Resource
    Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 3 ( 2022-01-19), p. 1068-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 3 ( 2022-01-19), p. 1068-
    Abstract: Due to their hydrophilic, biocompatible and adjustability properties, hydrogels have received a lot of attention. The introduction of nucleic acids has made hydrogels highly stimuli-responsiveness and they have become a new generation of intelligent biomaterials. In this review, the development and utilization of smart nucleic acid hydrogels (NAHs) with a high stimulation responsiveness were elaborated systematically. We discussed NAHs with a high stimuli-responsiveness, including pure NAHs and hybrid NAHs. In particular, four stimulation factors of NAHs were described in details, including pH, ions, small molecular substances, and temperature. The research progress of nucleic acid hydrogels in biomedical applications in recent years is comprehensively discussed. Finally, the opportunities and challenges facing the future development of nucleic acid hydrogels are also discussed.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms23031068
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Preparation of a CAB−GO/PES Mixed Matrix Ultrafiltration Membrane and Its Antifouling Performance
    MDPI AG ; 2023
    In:  Membranes Vol. 13, No. 2 ( 2023-02-17), p. 241-
    Details
    In: Membranes, MDPI AG, Vol. 13, No. 2 ( 2023-02-17), p. 241-
    Abstract: Serious membrane fouling has limited the development of ultrafiltration membrane technology for water purification. Synthesis of an ultrafiltration membrane with prominent anti-fouling ability is of vital importance. In this study, CAB−GO composite nanosheets were prepared by grafting graphene oxide (GO) with a zwitterionic material cocamidopropyl betaine (CAB) with strong antifouling properties. Anti-fouling CAB−GO/PES mixed matrix ultrafiltration membrane (CGM) was prepared by the phase inversion method with polyethersulfone (PES). Due to its electrostatic interaction, the interlayer distance between CAB−GO nanosheets was increased, and the dispersibility of GO was improved to large extent, thereby effectively avoiding the phenomenon of GO agglomeration in organic solvents. Based on the improvement of the surface porosity and surface hydrophilicity of the CAB−GO/PES mixed matrix membrane, the pure water flux of CGM−1.0 can reach 461 L/(m2·h), which was 2.5 times higher than that of the original PES membrane, and the rejection rates toward BSA and HA were above 96%. Moreover, when the content of CAB−GO was 0.1 wt%, the prepared CAB−GO/PES membrane exhibited very high BSA (99.1%) and HA (98.1%) rejection during long-term operation, indicating excellent anti-fouling ability.
    Type of Medium: Online Resource
    ISSN: 2077-0375
    URL: Article
    DOI: 10.3390/membranes13020241
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2614641-1
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  • 5
    Online Resource
    Online Resource
    A Novel Cu2+ Quantitative Detection Nucleic Acid Biosensors Based on DNAzyme and “Blocker” Beacon
    MDPI AG ; 2023
    In:  Foods Vol. 12, No. 7 ( 2023-04-03), p. 1504-
    Details
    In: Foods, MDPI AG, Vol. 12, No. 7 ( 2023-04-03), p. 1504-
    Abstract: In this paper, a “turn-off” biosensor for detecting copper (II) ions based on Cu2+-dependent DNAzyme and a “blocker” beacon were developed. Upon the copper ion being added, the Cu2+-dependent DNAzyme substrate strand was irreversibly cleaved, thereby blocking the occurrence of the ligation reaction and PCR, which inhibited the G-rich sequence from forming the G-quadruplex structure, efficiently reducing the detection signal. This method had the characteristics of strong specificity and high sensitivity compared with the existing method due to the application of ligation-dependent probe signal recognition and amplification procedures. Under the optimized conditions, this method proved to be highly sensitive. The signal decreased as the concentration of copper ions increased, exhibiting a linear calibration from 0.03125 μM to 0.5 μM and a limit of detection of 18.25 nM. Subsequently, the selectivity of this biosensor was verified to be excellent by testing different relevant metal ions. Furthermore, this detection system of copper (II) ions was successfully applied to monitor Cu2+ contained in actual water samples, which demonstrated the feasibility of the biosensor.
    Type of Medium: Online Resource
    ISSN: 2304-8158
    URL: Article
    DOI: 10.3390/foods12071504
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704223-6
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  • 6
    Online Resource
    Online Resource
    Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by Komagataeibacter nataicola
    MDPI AG ; 2023
    In:  Fermentation Vol. 9, No. 6 ( 2023-06-17), p. 575-
    Details
    In: Fermentation, MDPI AG, Vol. 9, No. 6 ( 2023-06-17), p. 575-
    Abstract: Nata de coco, known as bacterial cellulose (BC), has been given much attention in the food industry and biomaterial areas due to its specific properties such as low calorie content, high content of fiber, high purity and high biocompatibility. Komagataeibacter spp. are indispensable microorganisms for BC production due to their highly efficient production. Here, proteomics was applied to investigate the metabolism regulation mechanisms of BC yield improvements in K. nataicola Y19 by 48 ± 3% after ethanol supplementation. The results evidenced that differentially expressed proteins involved in the BC biosynthesis system, glycolytic pathway, TCA cycle and oxidative phosphorylation process were up-regulated. The proteins accelerated the BC biosynthesis by providing more energy and via intermediate metabolites. Furthermore, the elongation factor Tu, chaperone DnaK and translocase subunit SecB may be involved in the BC synthesis procedure by regulating electron transfer, hydrolysis of ATP and protein transformation. Moreover, the ethanol-enhanced BC biosynthesis may be associated with the decreased expression of endoglucanase. This research elucidates the proteomics mechanism of higher BC production based on ethanol addition, providing references for nata de coco production efficiency and the synthetic regulation of bacterial cellulose in the future.
    Type of Medium: Online Resource
    ISSN: 2311-5637
    URL: Article
    DOI: 10.3390/fermentation9060575
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2813985-9
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  • 7
    Online Resource
    Online Resource
    Flood Frequency Analysis Using Mixture Distributions in Light of Prior Flood Type Classification in Norway
    MDPI AG ; 2023
    In:  Remote Sensing Vol. 15, No. 2 ( 2023-01-09), p. 401-
    Details
    In: Remote Sensing, MDPI AG, Vol. 15, No. 2 ( 2023-01-09), p. 401-
    Abstract: The fundamental assumption of flood frequency analysis is that flood samples are generated by the same flood generation mechanism (FGM). However, flood events are usually triggered by the interaction of meteorological factors and watershed properties, which results in different FMGs. To solve this problem, researchers have put forward traditional two-component mixture distributions (TCMD-T) without clearly linking each component distribution to an explicit FGM. In order to improve the physical meaning of mixture distributions in seasonal snow-covered areas, the ratio of rainfall to flood volume (referred to as rainfall–flood ratio, RF) method was used to classify distinct FGMs. Thus, the weighting coefficient of each component distribution was determined in advance in the rainfall–flood ratio based TCMD (TCMD-RF). TCMD-RF model was applied to 34 basins in Norway. The results showed that flood types can be clearly divided into rain-on-snow-induced flood, snowmelt-induced flood and rainfall-induced flood. Moreover, the design flood and associated uncertainties were also estimated. It is found that TCMD-RF model can reduce the uncertainties of design flood by 20% compared with TCMD-T. The superiority of TCMD-RF is attributed to its clear classification of FGMs, thus determining the weighting coefficients without optimization and simplifying the parameter estimation procedure of mixture distributions.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    URL: Article
    DOI: 10.3390/rs15020401
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2513863-7
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  • 8
    Online Resource
    Online Resource
    A Review: Epigenetic Mechanism in Ochratoxin A Toxicity Studies
    MDPI AG ; 2017
    In:  Toxins Vol. 9, No. 4 ( 2017-03-23), p. 113-
    Details
    In: Toxins, MDPI AG, Vol. 9, No. 4 ( 2017-03-23), p. 113-
    Type of Medium: Online Resource
    ISSN: 2072-6651
    URL: Article
    DOI: 10.3390/toxins9040113
    Language: English
    Publisher: MDPI AG
    Publication Date: 2017
    detail.hit.zdb_id: 2518395-3
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  • 9
    Online Resource
    Online Resource
    Improved Differential Evolution Algorithm for Sensitivity Enhancement of Surface Plasmon Resonance Biosensor Based on Two-Dimensional Material for Detection of Waterborne Bacteria
    MDPI AG ; 2023
    In:  Biosensors Vol. 13, No. 6 ( 2023-05-31), p. 600-
    Details
    In: Biosensors, MDPI AG, Vol. 13, No. 6 ( 2023-05-31), p. 600-
    Abstract: Due to the large number of waterborne bacteria presenting in drinking water, their rapid and accurate identification has become a global priority. The surface plasmon resonance (SPR) biosensor with prism (BK7)-silver(Ag)-MXene(Ti3T2Cx)-graphene- affinity-sensing medium is examined in this paper, in which the sensing medium includes pure water, vibrio cholera (V. cholera), and escherichia coli (E. coli). For the Ag-affinity-sensing medium, the maximum sensitivity is obtained by E. coli, followed by V. cholera, and the minimum is pure water. Based on the fixed-parameter scanning (FPS) method, the highest sensitivity is 246.2 °/RIU by the MXene and graphene with monolayer, and with E. coli sensing medium. Therefore, the algorithm of improved differential evolution (IDE) is obtained. By the IDE algorithm, after three iterations, the maximum fitness value (sensitivity) of the SPR biosensor achieves 246.6 °/RIU by using the structure of Ag (61 nm)-MXene (monolayer)-graphene (monolayer)-affinity (4 nm)-E. coli. Compared with the FPS and differential evolution (DE) algorithm, the highest sensitivity is more accurate and efficient, and with fewer iterations. The performance optimization of multilayer SPR biosensors provides an efficient platform.
    Type of Medium: Online Resource
    ISSN: 2079-6374
    URL: Article
    DOI: 10.3390/bios13060600
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662125-3
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  • 10
    Online Resource
    Online Resource
    In-Situ Fabricating V2O5/TiO2-Carbon Heterojunction from Ti3C2 MXene as Highly Active Visible-Light Photocatalyst
    MDPI AG ; 2022
    In:  Nanomaterials Vol. 12, No. 10 ( 2022-05-23), p. 1776-
    Details
    In: Nanomaterials, MDPI AG, Vol. 12, No. 10 ( 2022-05-23), p. 1776-
    Abstract: Titanium dioxide is a mainstream photocatalyst, but it still confronts great obstacles of poor visible light absorption and rapid recombination rate of photogenerated carriers. Herein, we describe the design of a highly active visible-light photocatalytic system of graphited carbon layers anchored V2O5/TiO2 heterojunctions derived from Ti3C2 MXene, which demonstrates about 4.58 and 2.79 times higher degradation activity of MB under visible light (λ 〉 420 nm) than pure V2O5 and TiO2-carbon. Combined with the characterization results, the formed V2O5/TiO2 heterojunction promotes the separation of photogenerated carriers, while the graphitized carbon derived from MXene acts as an electronic reservoir to enhance the absorption of visible light. The ESR results show that superoxide radicals and hydroxyl radicals are the main active species in the reaction system. Therefore, we propose a possible mechanism model to provide a feasible idea for the subsequent design of high-efficiency photocatalysts for environmental treatment.
    Type of Medium: Online Resource
    ISSN: 2079-4991
    URL: Article
    DOI: 10.3390/nano12101776
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662255-5
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