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  • Wiley  (18)
  • Xu, Jilin  (18)
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  • 1
    Online Resource
    Online Resource
    Simultaneous analysis of ten low‐molecular‐mass organic acids in the tricarboxylic acid cycle and photorespiration pathway in Thalassiosira pseudonana at different growth stages
    Wiley ; 2017
    In:  Journal of Separation Science Vol. 40, No. 3 ( 2017-02), p. 635-645
    Details
    In: Journal of Separation Science, Wiley, Vol. 40, No. 3 ( 2017-02), p. 635-645
    Abstract: A method using high‐performance liquid chromatography coupled with tandem mass spectrometry was developed for the simultaneous determination of organic acids in microalgae. o ‐Benzylhydroxylamine was used to derivatize the analytes, and stable isotope‐labeled compounds were used as internal standards for precise quantification. The proposed method was evaluated in terms of linearity, recovery, matrix effect, sensitivity, and precision. Linear calibration curves with correlation coefficients 〉 0.99 were obtained over the concentration range of 0.4–40 ng/mL   for glycolic acid, 0.1–10 ng/mL for malic acid and oxaloacetic acid, 0.02–2 ng/mL for succinic acid and glyoxylic acid, 4–400 ng/mL for fumaric acid, 20–2000 ng/mL for isocitric acid, 2–200 ng mL −1  for citric acid, 100–10000 ng mL −1  for cis ‐aconitic acid, and 1–100 ng mL −1  for α‐ketoglutaric acid. Analyte recoveries were between 80.2 and 115.1%, and the matrix effect was minimal. Low limits of detection (0.003–1 ng/mL) and limits of quantification (0.01–5 ng/mL) were obtained except cis ‐aconitic acid. Variations in reproducibility for standard solution at three different concentrations levels were 〈 9%. This is the first report of the simultaneous analysis of ten organic acids in microalgae, which promotes better understanding of their growth state and provides reference value for high‐yield microalgae cultures.
    Type of Medium: Online Resource
    ISSN: 1615-9306 , 1615-9314
    URL: Issue
    URL: Article
    DOI: 10.1002/jssc.v40.3
    DOI: 10.1002/jssc.201600852
    RVK:
    VA 5450
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2047990-6
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  • 2
    Online Resource
    Online Resource
    Structural elucidation of two types of novel glycosphingolipids in three strains of Skeletonema by liquid chromatography coupled with mass spectrometry : Determination of glycosphingolipids in microalgae by LC/MS
    Wiley ; 2013
    In:  Rapid Communications in Mass Spectrometry Vol. 27, No. 13 ( 2013-07-15), p. 1535-1547
    Details
    In: Rapid Communications in Mass Spectrometry, Wiley, Vol. 27, No. 13 ( 2013-07-15), p. 1535-1547
    Type of Medium: Online Resource
    ISSN: 0951-4198
    URL: Article
    DOI: 10.1002/rcm.6602
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2002158-6
    detail.hit.zdb_id: 58731-X
    SSG: 11
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  • 3
    Online Resource
    Online Resource
    Cadmium transcriptionally regulates Scd1 expression in silver pomfret
    Wiley ; 2020
    In:  Environmental Toxicology Vol. 35, No. 3 ( 2020-03), p. 404-413
    Details
    In: Environmental Toxicology, Wiley, Vol. 35, No. 3 ( 2020-03), p. 404-413
    Abstract: Cadmium (Cd) is one of the major contaminants in aquatic ecosystem. Stearoyl‐coenzyme A desaturase 1 (Scd1) has been implicated in adaptive responses to environmental stressors. The objectives of this study are (a) to characterize scd1 mRNA from silver pomfret ( Pampus argenteus ); (b) to investigate the expression and activity of Scd1 in silver pomfret exposed to Cd; and (c) to investigate how Cd modifies scd1 gene transcription in silver pomfret. Results indicated that Scd1 was generally conserved across fish species and scd1 mRNA level was higher by far in the brain and liver, followed by the kidney and intestine. Exposure to Cd led to significant changes of the expression and activity of Scd1 in in the liver and intestine. The liver mRNA abundance of scd1 was significantly lower in the Cd‐treated groups than in the control group. The 10 days treatment with 1 mg/L Cd significantly upregulated the intestinal scd1 mRNA level, an approximately 9‐fold higher in the 1 mg/L Cd‐treated group as compared with the control group. Accordingly, Scd1 activity indices (18:1n‐9/18:0) in the liver were significantly decreased in the 0.5 mg/L group compared with the control group, while Scd1 activity indices in the intestine were significantly increased in the 1 mg/L group compared with the control group. Moreover, overexpression of sterol regulatory element binding transcription factor 1 (Srebp1) and peroxisome proliferator–activated receptor γ (Pparγ )in HEK 293T cells produced a 2‐fold increment in the activity of the scd1 promoter. Furthermore, srebp1 had a similar expression pattern to scd1 in the liver and intestine of silver pomfret exposed to Cd. These results indicated that Cd could regulate scd1 expression, possibly through the transcriptional factor Srebp1.
    Type of Medium: Online Resource
    ISSN: 1520-4081 , 1522-7278
    URL: Issue
    URL: Article
    DOI: 10.1002/tox.v35.3
    DOI: 10.1002/tox.22877
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2027534-1
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  • 4
    Online Resource
    Online Resource
    Simultaneous structural identification of diacylglyceryl‐ N ‐trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl‐ N,N,N ‐trimethyl‐β‐alanine (DGTA) in microalgae using dual Li + /H + adduct ion mode by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry
    Wiley ; 2017
    In:  Rapid Communications in Mass Spectrometry Vol. 31, No. 5 ( 2017-03-15), p. 457-468
    Details
    In: Rapid Communications in Mass Spectrometry, Wiley, Vol. 31, No. 5 ( 2017-03-15), p. 457-468
    Abstract: Diacylgycerol‐ N ‐trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl‐ N,N,N ‐trimethyl‐β‐alanine (DGTA) are structural isomers that are the most commonly described betaine lipids in microalgae. The structural differentiation and precise identification of DGTS and DGTA in microalgae need to be established during mass spectrometry analysis. Methods Total lipid was extracted from Amphora sp. with CHCl 3 /CH 3 OH (1:1, v/v). The qualitative analysis of DGTS and DGTA in Amphora sp. was carried out using Li + /H + dual mode by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry operating in MS E mode (UPLC/QTOF MS E ). Results Characteristic fragment ions [C 10 H 22 O 5 N] + at m/z 236.15 and [C 7 H 14 O 2 N] + at m/z 144.10 from the [M + H] + precursor ion can be used for the qualitative analysis of both DGTA and DGTS, whereas the loss of m/z 87 and 74 from the [M + Li] + precursor ion are specific for DGTS, and the loss of m/z 103 from the [M + Li] + precursor ion is only for DGTA. As a result, 9 DGTSs and 16 DGTAs with different fatty acids were identified simultaneously in Amphora sp. Semi‐quantitative analysis of DGTS and DGTA in Amphora sp. showed that the contents of DGTS ranged from 0.003 to 0.438 nmol mg –1 dw, and that of DGTA from 0.004 to 0.414 nmol mg –1 dw. Conclusions This is the first report to achieve the ambiguous structural identification of DGTS and DGTA by UPLC/QTOF MS E using dual Li + /H + adduct ion mode, which has remained a challenge in the past. It could provide new insights into their phylogeny and be helpful to characterize the natural phytoplankton communities as intact polar lipid biomarkers. Copyright © 2017 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0951-4198 , 1097-0231
    URL: Issue
    URL: Article
    DOI: 10.1002/rcm.v31.5
    DOI: 10.1002/rcm.7818
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2002158-6
    detail.hit.zdb_id: 58731-X
    SSG: 11
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  • 5
    Online Resource
    Online Resource
    Change of volatile components in six microalgae with different growth phases
    Wiley ; 2017
    In:  Journal of the Science of Food and Agriculture Vol. 97, No. 3 ( 2017-02), p. 761-769
    Details
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 97, No. 3 ( 2017-02), p. 761-769
    Abstract: Head space solid‐phase microextraction‐gas chromatography‐mass spectrometry has been applied to analyze the volatile components of six marine microalgae ( Thalassiosira weissflogii , Nitzschia closterium , Chaetoceros calcitrans , Platymonas helgolandica , Nannochloropsis spp . and Dicrateria inornata ) from Bacillariophyta , Chlorophyta and Chrysophyta , respectively, in different growth phases. RESULTS All volatile compounds were identified by database searching in the NIST08 Mass Spectral Library and analyzed by principal component analysis with SIMCA ‐P software (Umetrics, Umea, Sweden). The results clearly revealed that the volatile components of the six microalgae were significantly different in the exponential, stationary and declining phases. Aldehydes, alkanes, some esters and dimethyl sulfide significantly changed in different growth phases. CONCLUSION This is the first report on the comprehensive characteristics of volatile components in different microalgae and in different growth phases. The results may provide reference data for studies on the flavor of cultivated aquatic organism, odor formation in nature water, choice of feeding period and microalgae species selection for the artificial rearing of marine organisms. © 2016 Society of Chemical Industry
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    URL: Article
    DOI: 10.1002/jsfa.2017.97.issue-3
    DOI: 10.1002/jsfa.7794
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2001807-1
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  • 6
    Online Resource
    Online Resource
    Characterization of steryl glycosides in marine microalgae by gas chromatography–triple quadrupole mass spectrometry (GC–QQQ‐MS)
    Wiley ; 2018
    In:  Journal of the Science of Food and Agriculture Vol. 98, No. 4 ( 2018-03), p. 1574-1583
    Details
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 98, No. 4 ( 2018-03), p. 1574-1583
    Abstract: Steryl glycosides (SGs) are sterol conjugates found in various plants, especially in those making up human diets. It has been demonstrated that SGs have potential health benefits, and they could be used as food supplements in a variety of food matrixes. Marine microalgae are a potential resource for human food and ingredients. In this study, gas chromatography–triple quadrupole mass spectrometry (GC–QQQ‐MS) was used to characterize unknown SGs in eight microalgae belonging to different classes ( Isochrysis galbana 3011, Pavlova viridis , Platymonas helgolandica , Conticribra weissflogii , Thalassiosira pseudonana , Nitzschia closterium , Gymnodinium sp., and Karlodinum veneficum ). RESULTS The SGs were first extracted from lyophilized algae with chloroform–methanol, purified by solid‐phase extraction and analyzed as trimethylsilyl derivatives. Nine SGs have been identified. In particular, new SGs like occelasteryl glycoside and stellasteryl glycoside were found in Gymnodinium sp., 24‐methylene cholesteryl glycoside was detected in P. helgolandica , and 4,24‐dimethylcholestan‐3‐yl glycoside was identified as the main constituent of microalga K. veneficum . The results also showed that the compositions of SGs in different microalgae varied, with a range of 5.234 to 0.036 g kg −1 , and microalga P. viridis contained the most abundant SGs. CONCLUSION GC–QQQ‐MS is a powerful tool to detect SGs with different structures from a variety of microalgae. The compositions of SGs in different microalgae varied greatly. Microalgae are a good source of highly valued SGs. © 2017 Society of Chemical Industry
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    URL: Article
    DOI: 10.1002/jsfa.2018.98.issue-4
    DOI: 10.1002/jsfa.8629
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2001807-1
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  • 7
    Online Resource
    Online Resource
    Chromosome‐level genome assembly of the razor clam Sinonovacula constricta (Lamarck, 1818)
    Wiley ; 2019
    In:  Molecular Ecology Resources Vol. 19, No. 6 ( 2019-11), p. 1647-1658
    Details
    In: Molecular Ecology Resources, Wiley, Vol. 19, No. 6 ( 2019-11), p. 1647-1658
    Abstract: Bivalves, a highly diverse and the most evolutionarily successful class of invertebrates native to aquatic habitats, provide valuable molecular resources for understanding the evolutionary adaptation and aquatic ecology. Here, we reported a high‐quality chromosome‐level genome assembly of the razor clam Sinonovacula constricta using Pacific Bioscience single‐molecule real‐time sequencing, Illumina paired‐end sequencing, 10X Genomics linked‐reads and Hi‐C reads. The genome size was 1,220.85 Mb, containing scaffold N50 of 65.93 Mb and contig N50 of 976.94 Kb. A total of 899 complete (91.92%) and seven partial (0.72%) matches of the 978 metazoa Benchmarking Universal Single‐Copy Orthologs were determined in this genome assembly. And Hi‐C scaffolding of the genome resulted in 19 pseudochromosomes. A total of 28,594 protein‐coding genes were predicted in the S. constricta genome, of which 25,413 genes (88.88%) were functionally annotated. In addition, 39.79% of the assembled genome was composed of repetitive sequences, and 4,372 noncoding RNAs were identified. The enrichment analyses of the significantly expanded and contracted genes suggested an evolutionary adaptation of S. constricta to highly stressful living environments. In summary, the genomic resources generated in this work not only provide a valuable reference genome for investigating the molecular mechanisms of S. constricta biological functions and evolutionary adaptation, but also facilitate its genetic improvement and disease treatment. Meanwhile, the obtained genome greatly improves our understanding of the genetics of molluscs and their comparative evolution.
    Type of Medium: Online Resource
    ISSN: 1755-098X , 1755-0998
    URL: Issue
    URL: Article
    DOI: 10.1111/men.v19.6
    DOI: 10.1111/1755-0998.13086
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2406833-0
    SSG: 12
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  • 8
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    Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry
    Wiley ; 2016
    In:  Journal of Separation Science Vol. 39, No. 10 ( 2016-05), p. 1804-1813
    Details
    In: Journal of Separation Science, Wiley, Vol. 39, No. 10 ( 2016-05), p. 1804-1813
    Abstract: Phytohormones have attracted wide attention due to their important biological functions. However, their detection is still a challenge because of their complex composition, low abundance and diverse sources. In this study, a novel method of high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry was developed and validated for the simultaneous determination of ten phytohormones including indole‐3‐acetic acid, isopentenyladenine, isopentenyl adenosine, trans‐zeatin riboside, zeatin, strigolactones, abscisic acid, salicylic acid, gibberellin A3, and jasmonic acid in Sargassum horneri ( S. horneri ). The phytohormones were extracted from freeze‐dried S. horneri with methanol/water/methanoic acid (15:4:1, v/v/v) analyzed on a Hypersil Gold C 18 column and detected by electrospray ionization tandem triple quadrupole mass spectrometry in the multiple reaction monitoring mode. The experimental conditions for the extraction and analysis of phytohormones were optimized and validated in terms of reproducibility, linearity, sensitivity, recovery, accuracy, and stability. Distributions of the phytohormones in the stems, blades, and gas bladder of the S. horneri in drift, fixed, and semi‐fixed growing states were investigated for the first time. The observed contents of the phytohormones in S. horneri range from not detected to 5066.67 ng/g (fresh weight). Most phytohormones are distributed mainly in the stems of S. horneri in drift and semi‐fixed states.
    Type of Medium: Online Resource
    ISSN: 1615-9306 , 1615-9314
    URL: Issue
    URL: Article
    DOI: 10.1002/jssc.v39.10
    DOI: 10.1002/jssc.201501239
    RVK:
    VA 5450
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2047990-6
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  • 9
    Online Resource
    Online Resource
    Simultaneous determination of toxins in algae and water samples by high-performance liquid chromatography with triple quadrupole mass spectrometry : Liquid Chromatography
    Wiley ; 2012
    In:  Journal of Separation Science Vol. 35, No. 9 ( 2012-05), p. 1094-1101
    Details
    In: Journal of Separation Science, Wiley, Vol. 35, No. 9 ( 2012-05), p. 1094-1101
    Type of Medium: Online Resource
    ISSN: 1615-9306
    URL: Issue
    URL: Article
    DOI: 10.1002/jssc.v35.9
    DOI: 10.1002/jssc.201101069
    RVK:
    VA 5450
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2047990-6
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  • 10
    Online Resource
    Online Resource
    Structural elucidation of co‐eluted triglycerides in the marine diatom model organism Thalassiosira pseudonana by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry
    Wiley ; 2014
    In:  Rapid Communications in Mass Spectrometry Vol. 28, No. 3 ( 2014-02-15), p. 245-255
    Details
    In: Rapid Communications in Mass Spectrometry, Wiley, Vol. 28, No. 3 ( 2014-02-15), p. 245-255
    Abstract: The precise identification of fatty acids at the sn ‐2 position of triacylglycerols (TAGs), especially for positional regioisomers (AAB/ABA), needs to be established during mass spectrometry analysis. The detailed structural information about TAGs is significant not only for the assessment of biofuel quality, but also for the tracing of biosynthetic precursors. METHODS Total lipid was extracted from T. pseudonana by a modified Bligh and Dyer method. The qualitative analysis of TAGs in T. pseudonana was carried out using ultra‐performance liquid chromatography/electrospray ionization‐quadrupole time‐of‐flight mass spectrometry (UPLC/ESI‐Q‐TOF‐MS). The raw LC/MS data were analyzed using MassLynx software (version 4.1, Waters). RESULTS The acyl group at the sn ‐2 position of the TAGs has been identified unequivocally by [M + Li‐R 1/3 COOH‐R 2 CH = CHCOOH] + and the abundance of [M + Li‐R 1/3 COOH‐R 2 CH = CHCOOH] + can be used to confirm whether the TAG isomers are co‐eluted. In total, twelve TAGs were identified in T. pseudonana based on the fragmentation patterns discussed above. The data indicated that only C 16 fatty acids were located at the sn ‐2 position, which was important to trace the biosynthetic precursors of TAGs. CONCLUSIONS We put forward a hypothesis that TAGs in T. pseudonana are only derived from lipids in chloroplasts through prokaryotic biosynthesis pathway based on the precise information of sn ‐2 fatty acids, which is significant not only for the assessment of biofuel quality, but also for the tracing of biosynthetic precursors. Copyright © 2013 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0951-4198 , 1097-0231
    URL: Issue
    URL: Article
    DOI: 10.1002/rcm.v28.3
    DOI: 10.1002/rcm.6784
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 2002158-6
    detail.hit.zdb_id: 58731-X
    SSG: 11
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