In:
The FEBS Journal, Wiley, Vol. 283, No. 7 ( 2016-04), p. 1261-1274
Abstract:
Hepatic injury provoked by cold storage is a major problem affecting liver transplantation, as exposure to cold induces apoptosis in hepatic tissues. Long noncoding RNA s (lnc RNA s) are increasingly understood to regulate apoptosis, but the contribution of lnc RNA s to cold‐induced liver injury remains unknown. Using RNA ‐seq, we determined the differential lnc RNA expression profile in mouse livers after cold storage and found that expression of the lnc RNA TUG 1 was significantly down‐regulated. Overexpression of TUG 1 attenuated cold‐induced apoptosis in mouse hepatocytes and liver sinusoidal endothelial cells LSEC s, in part by blocking mitochondrial apoptosis and endoplasmic reticulum (ER) stress pathways. Moreover, TUG 1 attenuated apoptosis, inflammation, and oxidative stress in vivo in livers subjected to cold storage. Overexpression of TUG 1 also improved hepatocyte function and prolonged hepatic graft survival rates in mice. These results suggest that the lnc RNA TUG 1 exerts a protective effect against cold‐induced liver damage by inhibiting apoptosis in mice, and suggests a potential role for TUG 1 as a target for the prevention of cold‐induced liver damage in liver transplantation. Databases RNA‐seq data are available from GEO using accession number GSE76609 .
Type of Medium:
Online Resource
ISSN:
1742-464X
,
1742-4658
DOI:
10.1111/febs.2016.283.issue-7
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2172518-4
SSG:
12
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