In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 6 ( 2020-06-01), p. 3665-3672
Abstract:
Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136 T and dk3543, were isolated from the faeces of Tibetan gazelle ( Procapra picticaudata ) in the Qinghai–Tibet Plateau of PR China. The cells were aerobic, oxidase-negative and catalase-positive. Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain–heart infusion (BHI) agar with 5 % sheep blood. The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood. Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25 T (97.9 % similarity). The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25 T , Nocardioides gilvus XZ17 T , Nocardioides houyundeii 78 T and Nocardioides daphniae D287 T were their nearest phylogenetic neighbours. The DNA G+C contents of strains dk3136 T and dk3543 were 70.3 mol% and 70.4 mol%, respectively. Their genomes exhibit lower than threshold (95–96 %) average nucleotide identity to known species of the genus Nocardioides . ll -2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H 4 ) was the predominant respiratory quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The two strains had C 18 : 1 ω 9 c , iso -C 16 : 0 and C 17 : 1 ω 8 c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars. On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136 T and dk3543 represent a novel species in genus Nocardioides , for which the name Nocardioides jishulii sp. nov. is proposed. The type strain is dk3136 T (=CGMCC 4.7570 T =JCM 33496 T =KCTC 49314 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004218
Language:
English
Publisher:
Microbiology Society
Publication Date:
2020
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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