In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 1 ( 2023-1-27), p. e1011134-
Abstract:
Autophagy plays an important role in virus infection of the host, because viral components and particles can be degraded by the host’s autophagy and some viruses may be able to hijack and subvert autophagy for its benefit. However, details on the mechanisms that govern autophagy for immunity against viral infections or benefit viral survival remain largely unknown. Plant reoviruses such as southern rice black-streaked dwarf virus (SRBSDV), which seriously threaten crop yield, are only transmitted by vector insects. Here, we report a novel mechanism by which SRBSDV induces incomplete autophagy by blocking autophagosome-lysosome fusion, resulting in viral accumulation in gut epithelial cells of its vector, white-backed planthopper ( Sogatella furcifera ). SRBSDV infection leads to stimulation of the c-Jun N-terminal kinase (JNK) signaling pathway, which further activates autophagy. Mature and assembling virions were found close to the edge7 of the outer membrane of autophagosomes. Inhibition autophagy leads to the decrease of autophagosomes, which resulting in impaired maturation of virions and the decrease of virus titer, whereas activation of autophagy facilitated virus titer. Further, SRBSDV inhibited fusion of autophagosomes and lysosomes by interacting with lysosomal-associated membrane protein 1 (LAMP1) using viral P10. Thus, SRBSDV not only avoids being degrading by lysosomes, but also further hijacks these non-fusing autophagosomes for its subsistence. Our findings reveal a novel mechanism of reovirus persistence, which can explain why SRBSDV can be acquired and transmitted rapidly by its insect vector.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011134
DOI:
10.1371/journal.ppat.1011134.g001
DOI:
10.1371/journal.ppat.1011134.g002
DOI:
10.1371/journal.ppat.1011134.g003
DOI:
10.1371/journal.ppat.1011134.g004
DOI:
10.1371/journal.ppat.1011134.g005
DOI:
10.1371/journal.ppat.1011134.g006
DOI:
10.1371/journal.ppat.1011134.g007
DOI:
10.1371/journal.ppat.1011134.g008
DOI:
10.1371/journal.ppat.1011134.g009
DOI:
10.1371/journal.ppat.1011134.s001
DOI:
10.1371/journal.ppat.1011134.s002
DOI:
10.1371/journal.ppat.1011134.s003
DOI:
10.1371/journal.ppat.1011134.s004
DOI:
10.1371/journal.ppat.1011134.s005
DOI:
10.1371/journal.ppat.1011134.s006
DOI:
10.1371/journal.ppat.1011134.s007
DOI:
10.1371/journal.ppat.1011134.s008
DOI:
10.1371/journal.ppat.1011134.s009
DOI:
10.1371/journal.ppat.1011134.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2205412-1
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