In:
Virologica Sinica, Elsevier BV, Vol. 32, No. 6 ( 2017-12), p. 465-475
Abstract:
Feasible and effective cell models for hepatitis B virus (HBV) infection are
required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol
(DMSO/PEG), hNTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell
line (Huh7D hNTCP ) to overcome these limiting factors so
that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO / 4% PEG8000, and one
resistant cell line (Huh7D) was used to construct a stable hNTCP-expressing cell line (Huh7D hNTCP ) using a recombinant lentivirus system.
Then, the morphological characteristics and differentiation molecular markers of Huh7D hNTCP cells with or without DMSO treatment were
characterized. Finally, the susceptibility of Huh7D hNTCP cells to HBV infection was assessed. Our results showed that Huh7D cells were
resistant to 2.5% DMSO / 4% PEG8000, whereas the others were not. Huh7D hNTCP cells were established to express a high
level of hNTCP compared to liver extracts, and Huh7D hNTCP cells rapidly transformed into a non-dividing, well-differentiated polarized
phenotype under DMSO treatment. Huh7D hNTCP cells fully
supported the entire lifecycle of HBV infection. This cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the
discovery of antiviral drugs and vaccines.
Type of Medium:
Online Resource
ISSN:
1674-0769
,
1995-820X
DOI:
10.1007/s12250-017-3983-x
Language:
English
Publisher:
Elsevier BV
Publication Date:
2017
detail.hit.zdb_id:
2425817-9
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