In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_10 ( 2015-10-01), p. 3690-3696
Abstract:
A novel bacterial strain, designated THG-RS2O T , was isolated from fallow-land soil previously cultivated with Brassica oleracea in Yongin, South Korea. Cells were Gram-stain-negative, aerobic, non-motile rods, catalase- and oxidase-positive. Strain THG-RS2O T grew optimally at 25–37 °C, at pH 7.0 and in the absence of NaCl. 16S rRNA gene sequence analysis demonstrated that strain THG-RS2O T shows highest sequence similarity with Massilia kyonggiensis KACC 17471 T followed by Massilia aerilata KACC 12505 T , Massilia niastensis KACC 12599 T , Massilia tieshanensis KACC 14940 T and Massilia haematophila KCTC 32001 T . Levels of DNA–DNA relatedness between strain THG-RS2O T and the closest phylogenetic neighbours were below 55.0 % and the DNA G+C content of strain THG-RS2O T was 63.2 mol%. Major fatty acids were C 16 : 0 , cyclo-C 17 : 0 and summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ). The major respiratory quinone was identified as ubiquonone-8 and predominant polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Characterization by 16S rRNA gene sequence analysis, DNA–DNA hybridization, ubiquinone, polar lipid, fatty acid composition, and physiological and biochemical parameters revealed that strain THG-RS2O T represents a novel species of the genus Massilia . Hence, the present study describes a novel species for which the name Massilia arvi sp. nov. is proposed. The type strain is THG-RS2O T ( = KCTC 42609 T = CCTCC AB 2015115 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000477
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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