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  • 1
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2004
    In:  Canadian Journal of Botany Vol. 82, No. 12 ( 2004-12-01), p. 1730-1743
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 82, No. 12 ( 2004-12-01), p. 1730-1743
    Kurzfassung: Cryphonectria includes important tree pathogens as well as species believed to be saprophytes. Recent phylogenetic studies have concentrated on North American and southern hemisphere Cryphonectria spp., but little is known about Asian and European taxa. In this study we identify and differentiate among the species occurring in Europe, China, and Japan using morphological and phylogenetic comparisons among the Cryphonectria species. Phylogenetic comparisons were based on sequence data from the ribosomal ITS operon and two regions in the β-tubulin gene. Unknown Japanese and Chinese isolates showing different cultural features than those of Cryphonectria parasitica (Murrill) M.E. Barr from Japan and the USA, grouped with isolates of Cryphonectria nitschkei (G.H. Otth) M.E. Barr from Quercus spp. and Rhus javanica L. Isolates of Cryphonectria havanensis (Bruner) M.E. Barr from Quercus grosseserrata Blume, Castanopsis cuspidata Schottky, Pyrus sinensis Lindl., and Eucalyptus globulus Labill. also grouped in this phylogenetic clade. We propose that Cryphonectria nitschkei and the fungus that has been referred to as Cryphonectria havanensis in Japan should be treated as a single taxon. Phylogenetic and morphological data also suggest that there are two species currently representing Cryphonectria radicalis (Schwein.: Fr.) M.E. Barr in Europe. One of these species is similar to the type specimen of Cryphonectria radicalis, while the other species probably is new.Key words: Cryphonectria parasitica, Cryphonectria radicalis, Cryphonectria havanensis, Cryphonectria macrospora, Cryphonectria nitschkei, Diaporthales.
    Materialart: Online-Ressource
    ISSN: 0008-4026
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2004
    ZDB Id: 218116-2
    ZDB Id: 1481926-0
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    In: G3 Genes|Genomes|Genetics, Oxford University Press (OUP), Vol. 11, No. 9 ( 2021-09-06)
    Kurzfassung: Colletotrichum species are known to engage in unique sexual behaviors that differ significantly from the mating strategies of other filamentous ascomycete species. For example, most ascomycete fungi require the expression of both the MAT1-1-1 and MAT1-2-1 genes to induce sexual reproduction. In contrast, all isolates of Colletotrichum harbor only the MAT1-2-1 gene and yet, are capable of recognizing suitable mating partners and producing sexual progeny. The molecular mechanisms contributing to mating types and behaviors in Colletotrichum are, however, unknown. A comparative genomics approach analyzing 35 genomes, representing 31 Colletotrichum species and two Verticillium species, was used to elucidate a putative molecular mechanism underlying the unique sexual behaviors observed in Colletotrichum species. The existence of only the MAT1-2 idiomorph was confirmed across all species included in this study. Comparisons of the loci harboring the two mating pheromones and their cognate receptors revealed interesting patterns of gene presence and absence. The results showed that these genes have been lost multiple, independent times over the evolutionary history of this genus. These losses indicate that the pheromone pathway no longer plays an active role in mating type determination, suggesting an undiscovered mechanism by which mating partner recognition is controlled in these species. This further suggests that there has been a redirection of the underlying genetic mechanisms that regulate sexual development in Colletotrichum species. This research thus provides a foundation from which further interrogation of this topic can take place.
    Materialart: Online-Ressource
    ISSN: 2160-1836
    Sprache: Englisch
    Verlag: Oxford University Press (OUP)
    Publikationsdatum: 2021
    ZDB Id: 2629978-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 1998
    In:  Canadian Journal of Botany Vol. 76, No. 8 ( 1998-08-01), p. 1412-1417
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 76, No. 8 ( 1998-08-01), p. 1412-1417
    Kurzfassung: Sphaeropsis sapinea (Fr.) Dyko & Sutton is an opportunistic pathogen of various Pinus spp., causing severe shoot blight and dieback. Some isolates of S. sapinea display characteristics such as reduced virulence, reduced growth rate, lack of pigmentation, altered colony morphology, and suppressed conidiation. South African isolates of S. sapinea displaying a range of growth patterns, including reduced growth rate and atypical morphology, were screened for the presence of double-stranded RNA (dsRNA). They were also tested for relative virulence in pathogenicity tests. Double-stranded RNA was isolated by means of phenol extraction and cellulose chromatographic purification. A single species of dsRNA (± 4.3 kilobase pairs in size) was obtained from two slow-growing isolates and two isolates with more regular growth. The virulence of these dsRNA-containing isolates was tested on mature Pinus patula Schlecht. et Cham. trees. Although reduced virulence was positively correlated with slower growth in vitro, the presence of dsRNA could not be linked to either of these characteristics.Key words: double-stranded RNA, hypovirulence, Sphaeropsis sapinea.
    Materialart: Online-Ressource
    ISSN: 0008-4026
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 1998
    ZDB Id: 218116-2
    ZDB Id: 1481926-0
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    In: BMC Genomics, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2013), p. 605-
    Materialart: Online-Ressource
    ISSN: 1471-2164
    Sprache: Englisch
    Verlag: Springer Science and Business Media LLC
    Publikationsdatum: 2013
    ZDB Id: 2041499-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    National Inquiry Services Center (NISC) ; 2020
    In:  Southern Forests: a Journal of Forest Science Vol. 82, No. 3 ( 2020-11-16), p. 253-262
    In: Southern Forests: a Journal of Forest Science, National Inquiry Services Center (NISC), Vol. 82, No. 3 ( 2020-11-16), p. 253-262
    Materialart: Online-Ressource
    ISSN: 2070-2620 , 2070-2639
    Sprache: Englisch
    Verlag: National Inquiry Services Center (NISC)
    Publikationsdatum: 2020
    ZDB Id: 2942037-4
    ZDB Id: 2563580-3
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2002
    In:  Canadian Journal of Botany Vol. 80, No. 6 ( 2002-06-01), p. 590-596
    In: Canadian Journal of Botany, Canadian Science Publishing, Vol. 80, No. 6 ( 2002-06-01), p. 590-596
    Kurzfassung: Cryphonectria cubensis (Bruner) Hodges is the causal agent of an important stem canker disease of Eucalyptus. Previous phylogenetic studies based on sequence data have shown that C. cubensis is distinct from other species of Cryphonectria but that C. cubensis isolates reside in two distinct groups, consistent with geographical origin. Thus, isolates of C. cubensis from South America and South Africa grouped together but apart from those originating from Southeast Asia and Australia. These results were in contrast with the symptoms of Cryphonectria canker in South Africa, which are different from those observed elsewhere in the world. The aim of this study was to use more variable regions of the fungal genome to test whether South African isolates of C. cubensis are genetically distinct from those from other parts of the world. For this comparison, β-tubulin and histone H3 gene sequences were used. Specimens from South America, Southeast Asia, Australia, and South Africa were also compared morphologically. The phylogram emerging from the analysis indicated that South American and Southeast Asian Australian isolates resided in two well-resolved but closely related clades. However, isolates from South Africa were distinct from other groups. This is consistent with ecological aspects of the South African fungus, although no obvious morphological differences between the fungi from the various regions could be found. Our results suggest that the South African fungus represents a species distinct from C. cubensis occurring elsewhere in the world.Key words: β-tubulin, histone H3, molecular phylogeny, Cryphonectria cubensis.
    Materialart: Online-Ressource
    ISSN: 0008-4026
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2002
    ZDB Id: 218116-2
    ZDB Id: 1481926-0
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Online-Ressource
    Online-Ressource
    Elsevier BV ; 2002
    In:  Forest Ecology and Management Vol. 161, No. 1-3 ( 2002-5), p. 181-188
    In: Forest Ecology and Management, Elsevier BV, Vol. 161, No. 1-3 ( 2002-5), p. 181-188
    Materialart: Online-Ressource
    ISSN: 0378-1127
    Sprache: Englisch
    Verlag: Elsevier BV
    Publikationsdatum: 2002
    ZDB Id: 2016648-5
    ZDB Id: 751138-3
    SSG: 23
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    In: BioTechniques, Future Science Ltd, Vol. 65, No. 5 ( 2018-11), p. 253-257
    Kurzfassung: It is challenging to sequence and assemble genomes of obligate plant pathogens and microorganisms because of limited amounts of DNA, comparatively large genomes and high numbers of repeat regions. We sequenced the 1.2 gigabase genome of an obligate rust fungus, Austropuccinia psidii, the cause of rust on Myrtaceae, with a Chromium 10X library. This technology has mostly been applied for single-cell sequencing in immunological studies of mammals. We compared scaffolds of a genome assembled from the Chromium library with one assembled from combined paired-end and mate-pair libraries, sequenced with Illumina HiSeq. Chromium 10X provided a superior assembly, in terms of number of scaffolds, N50 and number of genes recovered. It required less DNA than other methods and was sequenced and assembled at a lower cost. Chromium sequencing could provide a solution to sequence and assemble genomes of obligate plant pathogens where the amount of available DNA is a limiting factor.
    Materialart: Online-Ressource
    ISSN: 0736-6205 , 1940-9818
    Sprache: Englisch
    Verlag: Future Science Ltd
    Publikationsdatum: 2018
    ZDB Id: 1496354-1
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    In: Forestry, Oxford University Press (OUP), Vol. 96, No. 4 ( 2023-07-08), p. 509-517
    Kurzfassung: Species of Corymbia are increasingly being tested for plantation establishment in South Africa. During disease surveys in KwaZulu-Natal plantations in 2020, a serious canker disease was found on Corymbia henryi. The cankers were seemingly caused by a fungus belonging to the family Cryphonectriaceae. The aims of the study were to identify the fungus causing the disease, to test its pathogenicity and to determine the genetic diversity of a population of isolates from infected trees. Phylogenetic analyses using sequence data for the internal transcribed spacer, β-tubulin 1 and β-tubulin 2 gene regions showed that the causal agent of the cankers was Chrysoporthe austroafricana. This is the first record of C. austroafricana causing cankers on a Corymbia species. A pathogenicity trial demonstrated that the fungus could infect C. henryi and cause cankers. Fifteen isolates of C. austroafricana were subjected to population genetic analyses using microsatellite markers. Eleven multilocus genotypes (MLGs) were detected. Mating-type distribution was approximately equal (MAT1-1:MAT1-2 = 8:7). The results indicate that a wide diversity of genotypes of C. austroafricana have undergone a host shift to infect C. henryi. This is a similar situation to that observed for Eucalyptus in South Africa and suggests that research efforts will be required to reduce the risk of Cryphonectria canker limiting afforestation using Corymbia species in the future.
    Materialart: Online-Ressource
    ISSN: 0015-752X , 1464-3626
    Sprache: Englisch
    Verlag: Oxford University Press (OUP)
    Publikationsdatum: 2023
    ZDB Id: 1466705-8
    SSG: 23
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
    Online-Ressource
    Online-Ressource
    National Inquiry Services Center (NISC) ; 2014
    In:  Southern Forests: a Journal of Forest Science Vol. 76, No. 3 ( 2014-07-03), p. 137-143
    In: Southern Forests: a Journal of Forest Science, National Inquiry Services Center (NISC), Vol. 76, No. 3 ( 2014-07-03), p. 137-143
    Materialart: Online-Ressource
    ISSN: 2070-2620 , 2070-2639
    Sprache: Englisch
    Verlag: National Inquiry Services Center (NISC)
    Publikationsdatum: 2014
    ZDB Id: 2942037-4
    ZDB Id: 2563580-3
    Standort Signatur Einschränkungen Verfügbarkeit
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