In:
Journal of Nanomaterials, Hindawi Limited, Vol. 2020 ( 2020-05-08), p. 1-11
Abstract:
In this paper, N, Fe-codoped carbon dots (N, Fe-CDs) were synthesized from β -cyclodextrin, ethylenediamine, and ferric chloride for the first time using a convenient one-step hydrothermal method. The obtained N, Fe-CDs were characterized by various methods including transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy. The N, Fe-CDs exhibited better catalytic activity than horseradish peroxidase (HRP) and caused an evident color change for 3,3 ′ ,5,5 ′ -tetramethylbenzidine in the presence of H 2 O 2 . Kinetic experiments show that the apparent K m value for the N, Fe-CDs with TMB (0.40 mM) or H 2 O 2 (0.35 mM) as the substrate was lower than that of HRP (0.43 and 3.70 mM), suggesting that the N, Fe-CDs have a much higher affinity for TMB and H 2 O 2 than HRP. The K m /V max value for the N, Fe-CDs ( 21.74 × 10 3 · s for H 2 O 2 ) is significantly lower than that for HRP ( 42.53 × 10 3 · s ), suggesting that the N, Fe-CDs have a stronger catalytic efficiency for H 2 O 2 than HRP. Furthermore, a highly efficient and sensitive colorimetric detection method for glucose was developed using the N, Fe-CDs as mimic peroxidase to detect the hydrogen peroxide generated by the oxidation of glucose by glucose oxidase. The limit of detection for H 2 O 2 and glucose was found to be 0.52 and 3.0 μ M, respectively. The obtained N, Fe-codoped carbon dots, which possess simulated peroxidase activity, can potentially be used in the field of biotechnology.
Type of Medium:
Online Resource
ISSN:
1687-4110
,
1687-4129
DOI:
10.1155/2020/1363212
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2020
detail.hit.zdb_id:
2229480-6
Permalink