In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 12 ( 2021-7-29)
Abstract:
Sesame ( Sesamum indicum L.) has always been known as a health-promoting oilseed crop because of its nutrient-rich oil. In recent years, studies have focused on lipid and fatty acid (FA) biosynthesis in various plants by high-throughput sequencing. Here, we integrated transcriptomics, small RNAs, and the degradome to establish a comprehensive reserve intensive on key regulatory micro RNA (miRNA)-targeting circuits to better understand the transcriptional and translational regulation of the oil biosynthesis mechanism in sesame seed development. Deep sequencing was performed to differentially express 220 miRNAs, including 65 novel miRNAs, in different developmental periods of seeds. GO and integrated KEGG analysis revealed 32 pairs of miRNA targets with negatively correlated expression profiles, of which 12 miRNA-target pairs were further confirmed by RT-PCR. In addition, a regulatory co-expression network was constructed based on the differentially expressed gene (DEG) profiles. The FAD2 , LOC10515945 , LOC105161564 , and LOC105162196 genes were clustered into groups that regulate the accumulation of unsaturated fatty acid (UFA) biosynthesis. The results provide a unique advanced molecular platform for the study of lipid and FA biosynthesis, and this study may serve as a new theoretical reference to obtain increased levels of UFA from higher-quality sesame seed cultivars and other plants.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2021.709197
DOI:
10.3389/fpls.2021.709197.s001
DOI:
10.3389/fpls.2021.709197.s002
DOI:
10.3389/fpls.2021.709197.s003
DOI:
10.3389/fpls.2021.709197.s004
DOI:
10.3389/fpls.2021.709197.s005
DOI:
10.3389/fpls.2021.709197.s006
DOI:
10.3389/fpls.2021.709197.s007
DOI:
10.3389/fpls.2021.709197.s008
DOI:
10.3389/fpls.2021.709197.s009
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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