In:
Annals of Nuclear Medicine, Springer Science and Business Media LLC, Vol. 28, No. 10 ( 2014-12), p. 961-969
Abstract:
Cerenkov-light imaging provides inherently high resolution because the light is emitted near the positron radionuclide. However, the magnitude for the high spatial resolution of Cerenkov-light imaging is unclear. Its potential molecular imaging applications also remain unclear. We developed an ultrahigh-resolution Cerenkov-light imaging system, measured its spatial resolution, and explored its applications to molecular imaging research. Methods Our Cerenkov-light imaging system consists of a high-sensitivity charged-coupled device camera (Hamamatsu Photonics ORCA2-ER) and a bright lens (Xenon 0.95/25). An extension ring was inserted between them to magnify the subject. A ~100-μm-diameter 22 Na point source was made and imaged by the system. For applications of Cerenkov-light imaging, we conducted 18 F-FDG administered in vivo, ex vivo whole brain, and sliced brain imaging of rats. Results We obtained spatial resolution of ~220 μm for a 22 Na point source with our developed imaging system. The 18 F-FDG rat head images showed high light intensity in the eyes for the Cerenkov-light images, although there was no accumulation in these parts in the PET images. The sliced rat brain showed much higher spatial resolution for the Cerenkov-light images compared with CdWO 4 scintillator-based autoradiography, although some contrast decrease was observed for them. Conclusion Even though the Cerenkov-light images showed ultrahigh resolution of ~220 μm, their distribution and contrast were sometimes different from the actual positron accumulation in the subjects. Care must be taken when evaluating positron distribution from Cerenkov-light images. However, the ultrahigh resolution of Cerenkov-light imaging will be useful for transparent subjects including phantom studies.
Type of Medium:
Online Resource
ISSN:
0914-7187
,
1864-6433
DOI:
10.1007/s12149-014-0892-z
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2014
detail.hit.zdb_id:
2039738-0
Permalink