In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 9 ( 2022-9-15), p. e0274530-
Abstract:
Trichome is important for help plant resist adversity and external damage. However, it often affects the appearance and taste of vegetables. In the present study, the trichome density of leaves from two Chinese cabbage cultivars with and without trichomes treated at low temperature are analyzed by biological microscope, and the differentially expressed genes related to trichomes formation were screened through transcriptome sequencing. The results showed that the number of leaves trichomes was reduced by 34.7% at low temperature compared with room temperature. A total of 661 differentially expression genes effecting trichomes formation were identified at the CT vs C, LCT vs LC, CT vs LCT. Several differentially expression genes from every comparison group were enriched in plant hormone signal transduction and amino acid biosynthesis pathway. Combined with the central genes obtained by WGCNA analysis, five candidate genes Bra029778 , Bra026393 , Bra030270 , Bra037264 and Bra009655 were screened. qRT-PCR analysis verified that the gene expression differences were in line with the trend of transcriptome data. This study not only found possible new key genes and laid a foundation for revealing the molecular mechanism regulating the formation of trichome in Chinese cabbage, but also provided a new way to study plant surface trichomes.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0274530
DOI:
10.1371/journal.pone.0274530.g001
DOI:
10.1371/journal.pone.0274530.g002
DOI:
10.1371/journal.pone.0274530.g003
DOI:
10.1371/journal.pone.0274530.g004
DOI:
10.1371/journal.pone.0274530.g005
DOI:
10.1371/journal.pone.0274530.g006
DOI:
10.1371/journal.pone.0274530.g007
DOI:
10.1371/journal.pone.0274530.g008
DOI:
10.1371/journal.pone.0274530.g009
DOI:
10.1371/journal.pone.0274530.g010
DOI:
10.1371/journal.pone.0274530.g011
DOI:
10.1371/journal.pone.0274530.t001
DOI:
10.1371/journal.pone.0274530.t002
DOI:
10.1371/journal.pone.0274530.s001
DOI:
10.1371/journal.pone.0274530.r001
DOI:
10.1371/journal.pone.0274530.r002
DOI:
10.1371/journal.pone.0274530.r003
DOI:
10.1371/journal.pone.0274530.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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