In:
Frontiers in Immunology, Frontiers Media SA, Vol. 14 ( 2023-6-26)
Abstract:
Tuberculosis (TB) is caused by Mycobacterium tuberculosis ( Mtb ) and remains a major health threat worldwide. However, a detailed understanding of the immune cells and inflammatory mediators in Mtb -infected tissues is still lacking. Tuberculous pleural effusion (TPE), which is characterized by an influx of immune cells to the pleural space, is thus a suitable platform for dissecting complex tissue responses to Mtb infection. Methods We employed singe-cell RNA sequencing to 10 pleural fluid (PF) samples from 6 patients with TPE and 4 non-TPEs including 2 samples from patients with TSPE (transudative pleural effusion) and 2 samples with MPE (malignant pleural effusion). Result Compared to TSPE and MPE, TPE displayed obvious difference in the abundance of major cell types (e.g., NK, CD4+T, Macrophages), which showed notable associations with disease type. Further analyses revealed that the CD4 lymphocyte population in TPE favored a Th1 and Th17 response. Tumor necrosis factors (TNF)-, and XIAP related factor 1 (XAF1)-pathways induced T cell apoptosis in patients with TPE. Immune exhaustion in NK cells was an important feature in TPE. Myeloid cells in TPE displayed stronger functional capacity for phagocytosis, antigen presentation and IFN-γ response, than TSPE and MPE. Systemic elevation of inflammatory response genes and pro-inflammatory cytokines were mainly driven by macrophages in patients with TPE. Conclusion We provide a tissue immune landscape of PF immune cells, and revealed a distinct local immune response in TPE and non-TPE (TSPE and MPE). These findings will improve our understanding of local TB immunopathogenesis and provide potential targets for TB therapy.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2023.1191357
DOI:
10.3389/fimmu.2023.1191357.s001
DOI:
10.3389/fimmu.2023.1191357.s002
DOI:
10.3389/fimmu.2023.1191357.s003
DOI:
10.3389/fimmu.2023.1191357.s004
DOI:
10.3389/fimmu.2023.1191357.s005
DOI:
10.3389/fimmu.2023.1191357.s006
DOI:
10.3389/fimmu.2023.1191357.s007
DOI:
10.3389/fimmu.2023.1191357.s008
DOI:
10.3389/fimmu.2023.1191357.s009
DOI:
10.3389/fimmu.2023.1191357.s010
DOI:
10.3389/fimmu.2023.1191357.s011
DOI:
10.3389/fimmu.2023.1191357.s012
DOI:
10.3389/fimmu.2023.1191357.s013
DOI:
10.3389/fimmu.2023.1191357.s014
DOI:
10.3389/fimmu.2023.1191357.s015
DOI:
10.3389/fimmu.2023.1191357.s016
DOI:
10.3389/fimmu.2023.1191357.s017
DOI:
10.3389/fimmu.2023.1191357.s018
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2606827-8
Permalink