In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-22), p. e0247418-
Abstract:
The increasing prevalence of carbapenem-resistant Acinetobacter baumannii (CRAB) caused nosocomial infections generate significant comorbidity and can cause death among patients. Current treatment options are limited. These infections pose great difficulties for infection control and clinical treatment. To identify the antimicrobial resistance, carbapenemases and genetic relatedness of Acinetobacter baumannii isolates from cerebrospinal fluid (CSF) and blood, a total of 50 nonrepetitive CSF isolates and 44 blood isolates were collected. The resistance phenotypes were determined, and polymerase chain reaction (PCR) was performed to examine the mechanisms of carbapenem resistance. Finally, multilocus sequence typing (MLST) was conducted to determine the genetic relatedness of these isolates. It was observed that 88 of the 94 collected isolates were resistant to imipenem or meropenem. Among them, the bla OXA-23 gene was the most prevalent carbapenemase gene, with an observed detection rate of 91.5% (86/94), followed by the bla OXA-24 gene with a 2.1% detection rate (2/94). Among all carbapenem-resistant Acinetobacter baumannii (CRAB) observations, isolates with the bla OXA-23 gene were resistant to both imipenem and meropenem. Interestingly, isolates positive for the bla OXA-24 gene but negative for the bla OXA-23 gene showed an imipenem-sensitive but meropenem-resistant phenotype. The MLST analysis identified 21 different sequence types (STs), with ST195, ST540 and ST208 most frequently detected (25.5%, 12.8% and 11.7%, respectively). 80 of the 94 isolates (85.1%) were clustered into CC92 which showed a carbapenem resistance phenotype (except AB13). Five novel STs were detected, and most of them belong to CRAB. In conclusion, these findings provide additional observations and epidemiological data of CSF and blood A . baumannii strains, which may improve future infection-control measures and aid in potential clinical treatments in hospitals and other clinical settings.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0247418
DOI:
10.1371/journal.pone.0247418.g001
DOI:
10.1371/journal.pone.0247418.g002
DOI:
10.1371/journal.pone.0247418.g003
DOI:
10.1371/journal.pone.0247418.t001
DOI:
10.1371/journal.pone.0247418.t002
DOI:
10.1371/journal.pone.0247418.t003
DOI:
10.1371/journal.pone.0247418.t004
DOI:
10.1371/journal.pone.0247418.s001
DOI:
10.1371/journal.pone.0247418.s002
DOI:
10.1371/journal.pone.0247418.s003
DOI:
10.1371/journal.pone.0247418.s004
DOI:
10.1371/journal.pone.0247418.s005
DOI:
10.1371/journal.pone.0247418.s006
DOI:
10.1371/journal.pone.0247418.r001
DOI:
10.1371/journal.pone.0247418.r002
DOI:
10.1371/journal.pone.0247418.r003
DOI:
10.1371/journal.pone.0247418.r004
DOI:
10.1371/journal.pone.0247418.r005
DOI:
10.1371/journal.pone.0247418.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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