In:
International Journal of Food Science & Technology, Wiley, Vol. 49, No. 3 ( 2014-03), p. 673-678
Abstract:
The salt‐tolerant T orulopsis versatilis mutant strain, T3‐5, is able to grow in YPD medium containing 22% salt, whereas the original strain, T , used for soy sauce fermentation was only able to grow in the presence of 20% or less. Biochemical analyses showed that strain T 3‐5 had a 22% higher intracellular glycerol level than T . Sequencing of the glycerol transporter gene FPS 1 revealed a change from G to A at position 198 upstream of the start codon, changes from G to A at positions 93 and 1084, respectively, within the FPS 1 gene, and insertions of T base at positions 285 and 310, respectively, downstream of the termination codon when comparing the corresponding sequences of strains T and T 3‐5. An inverse relationship between osmotic pressure and the FPS 1 transcription level was observed for both T and T 3‐5 using q PCR . Therefore, it is possible that lower transcription levels of FPS 1 are at least partly responsible for increased osmotolerance in strain T 3‐5. Overexpression of T and T 3‐5 FPS 1 in S accharomyces cerevisiae resulted in lower salt tolerance, especially in the presence of 12% salt. The data suggest that decreased expression levels of and/or alteration in the FPS 1 protein contributed to increased salt tolerance of T 3‐5.
Type of Medium:
Online Resource
ISSN:
0950-5423
,
1365-2621
DOI:
10.1111/ijfs.2014.49.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2016518-3
detail.hit.zdb_id:
2401430-8
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