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  • MDPI AG  (58)
  • Wang, Jie  (58)
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  • MDPI AG  (58)
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  • 1
    Online Resource
    Online Resource
    Immunoinformatic-Based Multi-Epitope Vaccine Design for Co-Infection of Mycobacterium tuberculosis and SARS-CoV-2
    MDPI AG ; 2023
    In:  Journal of Personalized Medicine Vol. 13, No. 1 ( 2023-01-05), p. 116-
    Details
    In: Journal of Personalized Medicine, MDPI AG, Vol. 13, No. 1 ( 2023-01-05), p. 116-
    Abstract: (1) Background: Many co-infections of Mycobacterium tuberculosis (MTB) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) have emerged since the occurrence of the SARS-CoV-2 pandemic. This study aims to design an effective preventive multi-epitope vaccine against the co-infection of MTB and SARS-CoV-2. (2) Methods: The three selected proteins (spike protein, diacylglycerol acyltransferase, and low molecular weight T-cell antigen TB8.4) were predicted using bioinformatics, and 16 epitopes with the highest ranks (10 helper T lymphocyte epitopes, 2 CD8+ T lymphocytes epitopes, and 4 B-cell epitopes) were selected and assembled into the candidate vaccine referred to as S7D5L4. The toxicity, sensitization, stability, solubility, antigenicity, and immunogenicity of the S7D5L4 vaccine were evaluated using bioinformatics tools. Subsequently, toll-like receptor 4 docking simulation and discontinuous B-cell epitope prediction were performed. Immune simulation and codon optimization were carried out using immunoinformatics and molecular biology tools. (3) Results: The S7D5L4 vaccine showed good physical properties, such as solubility, stability, non-sensitization, and non-toxicity. This vaccine had excellent antigenicity and immunogenicity and could successfully simulate immune responses in silico. Furthermore, the normal mode analysis of the S7D5L4 vaccine and toll-like receptor 4 docking simulation demonstrated that the vaccine had docking potential and a stable reaction. (4) Conclusions: The S7D5L4 vaccine designed to fight against the co-infection of MTB and SARS-CoV-2 may be safe and effective. The protective efficacy of this promising vaccine should be further verified using in vitro and in vivo experiments.
    Type of Medium: Online Resource
    ISSN: 2075-4426
    URL: Article
    DOI: 10.3390/jpm13010116
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662248-8
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  • 2
    Online Resource
    Online Resource
    An Abattoir-Based Study on the Prevalence of Salmonella Fecal Carriage and ESBL Related Antimicrobial Resistance from Culled Adult Dairy Cows in Wuhan, China
    MDPI AG ; 2020
    In:  Pathogens Vol. 9, No. 10 ( 2020-10-19), p. 853-
    Details
    In: Pathogens, MDPI AG, Vol. 9, No. 10 ( 2020-10-19), p. 853-
    Abstract: The objective of this study was to estimate the fecal carriage of Salmonella spp. among culled adult dairy cows presented to an abattoir in Wuhan, China and to evaluate their antimicrobial resistance profiles. Rectal swabs from 138 culled cows were cultured. Laboratory analysis involved the identification of Salmonella, the susceptibility assessment and the presence of Extended Spectrum β-lactamases and mcr genes in the isolates. An overall prevalence of Salmonella of 29.0% was recorded with 63.4% (26/41) and 2.4% (1/41) of the isolates identified as S. Typhimurium and S. Dublin, respectively. The occurrence of Salmonella was higher (odd ratios: 3.3) in culled cows originating from the northeast zone of China than cows originating from the central and north zones. Twenty multi-drug resistant strains (resistant to three or more antimicrobial agents) were detected (48.8%) and overall, a high resistance to ampicillin (36/41) and tetracycline (15/41) was observed. Extended Spectrum β-lactamases phenotypes were found in 7/41 isolates, of which all contained the blaCTX-M resistance gene, and no mcr genes were found by polymerase chain reaction. The high prevalence of Salmonella fecal carriage and antimicrobial resistance may contribute to an increased risk of Salmonella transmission to food.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    URL: Article
    DOI: 10.3390/pathogens9100853
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2695572-6
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  • 3
    Online Resource
    Online Resource
    Assessment of Multi-Source Evapotranspiration Products over China Using Eddy Covariance Observations
    MDPI AG ; 2018
    In:  Remote Sensing Vol. 10, No. 11 ( 2018-10-26), p. 1692-
    Details
    In: Remote Sensing, MDPI AG, Vol. 10, No. 11 ( 2018-10-26), p. 1692-
    Abstract: As an essential variable in linking water, carbon, and energy cycles, evapotranspiration (ET) is difficult to measure. Remote sensing, reanalysis, and land surface model-based ET products offer comprehensive alternatives at different spatio-temporal intervals, but their performance varies. In this study, we selected four popular ET global products: The Global Land Evaporation Amsterdam Model version 3.0a (GLEAM3.0a), the Modern Era Retrospective-Analysis for Research and Applications-Land (MERRA-Land) project, the Global Land Data Assimilation System version 2.0 with the Noah model (GLDAS2.0-Noah) and the EartH2Observe ensemble (EartH2Observe-En). Then, we comprehensively evaluated the performance of these products over China using a stratification method, six validation criteria, and high-quality eddy covariance (EC) measurements at 12 sites. The aim of this research was to provide important quantitative information to improve and apply the ET models and to inform choices about the appropriate ET product for specific applications. Results showed that, within one stratification, the performance of each ET product based on a certain criterion differed among classifications of this stratification. Furthermore, the optimal ET (OET) among these products was identified by comparing the magnitudes of each criterion. Results suggested that, given a criterion (a stratification classification), the OETs varied among stratification classifications (the selected six criteria). In short, no product consistently performed best, according to the selected validation criterion. Thus, multi-source ET datasets should be employed in future studies to enhance confidence in ET-related conclusions.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    URL: Article
    DOI: 10.3390/rs10111692
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2513863-7
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  • 4
    Online Resource
    Online Resource
    CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants
    MDPI AG ; 2023
    In:  International Journal of Molecular Sciences Vol. 24, No. 7 ( 2023-03-27), p. 6288-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 24, No. 7 ( 2023-03-27), p. 6288-
    Abstract: Photosystem II repair in chloroplasts is a critical process involved in maintaining a plant’s photosynthetic activity under cold stress. FtsH (filamentation temperature-sensitive H) is an essential metalloprotease that is required for chloroplast photosystem II repair. However, the role of FtsH in tea plants and its regulatory mechanism under cold stress remains elusive. In this study, we cloned a FtsH homolog gene in tea plants, named CsFtsH5, and found that CsFtsH5 was located in the chloroplast and cytomembrane. RT-qPCR showed that the expression of CsFtsH5 was increased with leaf maturity and was significantly induced by light and cold stress. Transient knockdown CsFtsH5 expression in tea leaves using antisense oligonucleotides resulted in hypersensitivity to cold stress, along with higher relative electrolyte leakage and lower Fv/Fm values. To investigate the molecular mechanism underlying CsFtsH5 involvement in the cold stress, we focused on the calcineurin B-like-interacting protein kinase 11 (CsCIPK11), which had a tissue expression pattern similar to that of CsFtsH5 and was also upregulated by light and cold stress. Yeast two-hybrid and dual luciferase (Luc) complementation assays revealed that CsFtsH5 interacted with CsCIPK11. Furthermore, the Dual-Luc assay showed that CsCIPK11-CsFtsH5 interaction might enhance CsFtsH5 stability. Altogether, our study demonstrates that CsFtsH5 is associated with CsCIPK11 and plays a positive role in maintaining the photosynthetic activity of tea plants in response to low temperatures.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms24076288
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Human Leukocyte Antigen Class II Alleles Are Associated with Hepatitis C Virus Natural Susceptibility in the Chinese Population
    MDPI AG ; 2015
    In:  International Journal of Molecular Sciences Vol. 16, No. 8 ( 2015-07-23), p. 16792-16805
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 16, No. 8 ( 2015-07-23), p. 16792-16805
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms160816792
    Language: English
    Publisher: MDPI AG
    Publication Date: 2015
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    PP19128R, a Multiepitope Vaccine Designed to Prevent Latent Tuberculosis Infection, Induced Immune Responses In Silico and In Vitro Assays
    MDPI AG ; 2023
    In:  Vaccines Vol. 11, No. 4 ( 2023-04-17), p. 856-
    Details
    In: Vaccines, MDPI AG, Vol. 11, No. 4 ( 2023-04-17), p. 856-
    Abstract: Background: Latent tuberculosis infection (LTBI) is the primary source of active tuberculosis (ATB), but a preventive vaccine against LTBI is lacking. Methods: In this study, dominant helper T lymphocyte (HTL), cytotoxic T lymphocyte (CTL), and B-cell epitopes were identified from nine antigens related to LTBI and regions of difference (RDs). These epitopes were used to construct a novel multiepitope vaccine (MEV) based on their antigenicity, immunogenicity, sensitization, and toxicity. The immunological characteristics of the MEV were analyzed with immunoinformatics technology and verified by enzyme-linked immunospot assay and Th1/Th2/Th17 cytokine assay in vitro. Results: A novel MEV, designated PP19128R, containing 19 HTL epitopes, 12 CTL epitopes, 8 B-cell epitopes, toll-like receptor (TLR) agonists, and helper peptides, was successfully constructed. Bioinformatics analysis showed that the antigenicity, immunogenicity, and solubility of PP19128R were 0.8067, 9.29811, and 0.900675, respectively. The global population coverage of PP19128R in HLA class I and II alleles reached 82.24% and 93.71%, respectively. The binding energies of the PP19128R-TLR2 and PP19128R-TLR4 complexes were −1324.77 kcal/mol and −1278 kcal/mol, respectively. In vitro experiments showed that the PP19128R vaccine significantly increased the number of interferon gamma-positive (IFN-γ+) T lymphocytes and the levels of cytokines, such as IFN-γ, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-10. Furthermore, positive correlations were observed between PP19128R-specific cytokines in ATB patients and individuals with LTBI. Conclusions: The PP19128R vaccine is a promising MEV with excellent antigenicity and immunogenicity and no toxicity or sensitization that can induce robust immune responses in silico and in vitro. This study provides a vaccine candidate for the prevention of LTBI in the future.
    Type of Medium: Online Resource
    ISSN: 2076-393X
    URL: Article
    DOI: 10.3390/vaccines11040856
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2703319-3
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  • 7
    Online Resource
    Online Resource
    Study on Infiltration and Soil Moisture Characteristics of a Sand-Covered Slope
    MDPI AG ; 2022
    In:  Water Vol. 14, No. 7 ( 2022-03-25), p. 1043-
    Details
    In: Water, MDPI AG, Vol. 14, No. 7 ( 2022-03-25), p. 1043-
    Abstract: By observing the processes of infiltration, flow generation, water flow characteristics and the spatial distribution of erosion in a designed rainfall test, and analyzing the infiltration, flow generation characteristics, water content change characteristics, soil moisture parameters change characteristics, and changes in the spatial patterns of erosion and sediment yield, this study draws the following conclusions: under different rainfall densities, the initial runoff generation time of sand-covered slope is 1~12 min longer than that of loess slope, the initial soil infiltration velocity of sediment-covered slope is about 1.23 times that of loess slope, and the time to reach stable infiltration of loess slope is shorter than that of sediment-covered slope. Under different rainfall densities, the rising time of the water content curve of sand-covered slope is earlier than that of loess slope. For the same duration of rainfall, the vertical infiltration performance of soil water of sand-covered slope is higher than that of loess slope, and when the rainfall density on the slope increases by 0.5 mm/min, the increase in runoff shear stress of the sediment-covered slope is about 1.5 times that of the loess slope, and the runoff power is about 1.13 times that of the loess slope.
    Type of Medium: Online Resource
    ISSN: 2073-4441
    URL: Article
    DOI: 10.3390/w14071043
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2521238-2
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  • 8
    Online Resource
    Online Resource
    Influence of Cooling Water Parameters on the Thermal Performance of the Secondary Circuit System of a Modular High-Temperature Gas-Cooled Reactor Nuclear Power Plant
    MDPI AG ; 2023
    In:  Energies Vol. 16, No. 18 ( 2023-09-12), p. 6560-
    Details
    In: Energies, MDPI AG, Vol. 16, No. 18 ( 2023-09-12), p. 6560-
    Abstract: This study quantitatively analysed the influence of cooling water parameters on the performance of a modular high-temperature gas-cooled reactor (MHTGR) nuclear power plant (NPP). The secondary circuit system and cold-end system were modelled using EBSILON software, version 16.0. The influence of cooling water inlet temperature and mass flow rate on the thermal performance of the secondary circuit system was analysed over the full power range with the goal of optimising net power. Under 100% rated condition, for each 1 °C increase in cooling water inlet temperature between 10 and 33 °C, the net power and cycle efficiency decreased by 0.67 MW and 0.14%, respectively, whereas the heat consumption rate increased by 28.72 kJ/(kW·h). The optimal cooling water mass flow rates corresponding to cooling water inlet temperatures of 16 °C and 33 °C were obtained. The optimal cooling water mass flow rate decreased nonlinearly with decreasing power levels. At a cooling water inlet temperature of 33 °C, an increase in cooling water mass flow rate from the designed value (7697.61 kg/s) to the optimal value (10,922.14 kg/s) resulted in a 1.03 MW increase in net power. These findings provide guidelines for MHTGR NPP operation optimisation and economic improvement, especially under high-temperature weather conditions.
    Type of Medium: Online Resource
    ISSN: 1996-1073
    URL: Article
    DOI: 10.3390/en16186560
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2437446-5
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  • 9
    Online Resource
    Online Resource
    Enhanced Piezoresponse and Dielectric Properties for Ba1-XSrXTiO3 Composition Ultrathin Films by the High-Throughput Method
    MDPI AG ; 2021
    In:  Coatings Vol. 11, No. 12 ( 2021-12-03), p. 1491-
    Details
    In: Coatings, MDPI AG, Vol. 11, No. 12 ( 2021-12-03), p. 1491-
    Abstract: The stacked single-unit cell Ba1-xSrxTiO3 (BSTO) thin film designed by the high-throughput method is fabricated by layer-by-layer deposition by laser molecular beam epitaxy, and its ferroelectric and dielectric characteristics as a function of Sr concentration are comprehensively investigated. The permittivity of BSTO exhibits a monotonous increase by Sr with a plateau in the region of 14% 〈 Sr 〈 85%. Meanwhile, at the low Sr doping regime, the piezoelectric response has been discovered, and the maximum piezoresponse and d33 can reach approximately 139.05 pm and 88 pm/V once an appropriate Ba/Sr ratio is formed, exhibiting a coexistence of a dielectric property and giant piezoresponse. This effective piezoelectric constant d33 value is significantly larger than the conventional chemical doping scenarios, suggesting that the intra-plane interaction is crucial for designing future promising dielectric and ferroelectric thin films via high-throughput technologies.
    Type of Medium: Online Resource
    ISSN: 2079-6412
    URL: Article
    DOI: 10.3390/coatings11121491
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2662314-6
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  • 10
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    Online Resource
    Evaluation of the Refined Decomposition Effect of Reclaimed Asphalt Pavement Materials
    MDPI AG ; 2023
    In:  Buildings Vol. 13, No. 9 ( 2023-09-04), p. 2240-
    Details
    In: Buildings, MDPI AG, Vol. 13, No. 9 ( 2023-09-04), p. 2240-
    Abstract: To improve the quality of reclaimed asphalt pavement materials (RAPs) and reduce the variability of RAPs, a refined decomposition process was applied to treat RAPs. The change rule and variability of aggregate gradation, asphalt content, aged asphalt properties and aggregate (extracted) properties of RAPs were analyzed with different frequency parameters. In addition, the gradation variability control method and the asphalt content variability control method were used to calculate the maximum addition proportion of RAPs according to the quality control requirements of hot-mixing asphalt mixtures. The results indicate that the variability of aggregate gradation, asphalt content and asphalt properties significantly reduced for the refined decomposition RAP. Compared with the original RAP (0 Hz) of 4.75–9.5 mm and 9.5–19 mm, the “false particle” content of the refined decomposition RAP (50 Hz) reduced by 75.6% and 64.3%, respectively. The refined decomposition process is conducive to the road performance of recycled asphalt mixtures, especially the dynamic stability and the maximum bending tensile strain. Comparing the recycled asphalt mixture with the original RAP (0 Hz), the maximum bending strain of the recycled asphalt mixture with the refined decomposition RAP (50 Hz) increased by 69%, and the immersion residual stability increased to 87.9%. The refined decomposition process improves the quality of the RAP and provides a reference for recycled asphalt mixtures with a high proportion of RAPs. This study contributes to RAP high-level recycling and carbon reduction in the highway maintenance industry.
    Type of Medium: Online Resource
    ISSN: 2075-5309
    URL: Article
    DOI: 10.3390/buildings13092240
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2661539-3
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