In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 17, No. 4 ( 2023-4-24), p. e0011254-
Abstract:
Rabies, caused by the rabies virus (RABV), is an ancient and neglected zoonotic disease posing a large public health threat to humans and animals in developing countries. Immunization of animals with a rabies vaccine is the most effective way to control the epidemic and the occurrence of the disease in humans. Therefore, the development of cost-effective and efficient rabies vaccines is urgently needed. The activation of dendritic cells (DCs) is known to play an important role in improving the host immune response induced by rabies vaccines. Methodology/Principal findings In this study, we constructed a recombinant virus, rCVS11-MAB2560, based on the reverse genetic system of the RABV CVS11 strain. The MAB2560 protein (a DC-targeting molecular) was chimeric expressed on the surface of the viral particles to help target and activate the DCs when this virus was used as inactivated vaccine. Our results demonstrated that inactivated rCVS11-MAB2560 was able to promote the recruitment and/or proliferation of DC cells, T cells and B cells in mice, and induce good immune memory after two immunizations. Moreover, the inactivated recombinant virus rCVS11-MAB2560 could produce higher levels of virus-neutralizing antibodies (VNAs) in both mice and dogs more quickly than rCVS11 post immunization. Conclusions/Significance In summary, the recombinant virus rCVS11-MAB2560 chimeric-expressing the molecular adjuvant MAB2560 can stimulate high levels of humoral and cellular immune responses in vivo and can be used as an effective inactivated rabies vaccine candidate.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0011254
DOI:
10.1371/journal.pntd.0011254.g001
DOI:
10.1371/journal.pntd.0011254.g002
DOI:
10.1371/journal.pntd.0011254.g003
DOI:
10.1371/journal.pntd.0011254.g004
DOI:
10.1371/journal.pntd.0011254.g005
DOI:
10.1371/journal.pntd.0011254.g006
DOI:
10.1371/journal.pntd.0011254.g007
DOI:
10.1371/journal.pntd.0011254.g008
DOI:
10.1371/journal.pntd.0011254.g009
DOI:
10.1371/journal.pntd.0011254.s001
DOI:
10.1371/journal.pntd.0011254.s002
DOI:
10.1371/journal.pntd.0011254.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2429704-5
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