In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_12 ( 2014-12-01), p. 4021-4026
Abstract:
An alkaliphilic actinobacterial strain, designated EGI 80099 T , was isolated from a rhizosphere soil sample of Reaumuria soongorica found in the desert soils of Fukang, Xinjiang, north-west China. Cells of strain EGI 80099 T were Gram-stain-positive, non-motile, non-endospore-forming cocci. The predominant menaquinones were MK-7, MK-8 and MK-9. The major cellular fatty acids ( 〉 10 %) were anteiso-C 15 : 0 and anteiso-C 17 : 0 . Analysis of the cell wall showed the presence of peptidoglycan of the type l -Lys–Gly– l -Glu, variation A4α. Cells of the isolate contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown phospholipid and an unidentified glycolipid as polar lipids. The genomic DNA G+C content was 63.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80099 T belongs to the genus Nesterenkonia , sharing 95.68–97.37 % sequence similarities with the type strains of recognized species within this genus. DNA–DNA hybridization of strain EGI 80099 T with the type strains of species that showed the highest sequence similarities, Nesterenkonia aethiopica DSM 17733 T (97.37 %), Nesterenkonia flava CAAS 251 T (97.23 %) and Nesterenkonia xinjiangensis YIM 70097 T (97.02 %), gave relatedness values of 8.7–62.2 %. Data from DNA–DNA hybridizations and physiological and biochemical tests indicated that strain EGI 80099 T represents a novel species of the genus Nesterenkonia , for which the name Nesterenkonia rhizosphaerae sp. nov. is proposed. The type strain is EGI 80099 T ( = BCRC 16947 T = JCM 19129 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.066894-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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