In:
The Journal of Immunology, The American Association of Immunologists, Vol. 186, No. 1_Supplement ( 2011-04-01), p. 66.36-66.36
Abstract:
A new subset of NKT cells, an IL-17-producing iNKT cell (iNKT17), has been identified recently. The role of this new iNKT17 subset in a tumor bearing host is unknown. We report that the percentages of iNKT17 cells in the thymus are increased dramatically in naïve mice from 0.5 ± 0.1% to 2.1 ± 0.2% in 4T1 breast tumor-bearing mice. Tumor induced iNKT17 cells are marked as TCRβint CD1dTetramerpos CD4neg CD8neg CD44hi CD25hi CD5pos CD24low DX5neg CD54pos. The iNKT17 cells from 4T1 tumor bearing mice are unique in that upon α-Gelcer-CD1d tetramer stimulation, IL-17 but not IL-4, IFN-γ, IL-10, IL-13, IL-6, IL-12 is induced. The CD11b+Gr1+ myeloid-derived suppressor cells (MDSC) accumulate in dramatic fashion in the thymus in 4T1 tumor bearing mice. The induction of iNKT17 subset is MDSC dependent. Addition of antibodies against TGFβ or IL-6 leads to the inhibition of MDSC-mediated induction of iNKT17 cells, suggesting that TGFβ and IL-6 are required for the induction. The MDSC-mediated induction of the iNKT17 cell subset is correlated with reduction of SLAM but not Ly9, CD84, Ly108 and 2B4. Mice injected intravenously with anti-IL17 antibody leads to the attenuation of 4T1 tumor metastasis to lung. This data suggest that MDSC-mediated induction of iNKT17 cells may play a role in promoting tumor metastasis.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.186.Supp.66.36
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2011
detail.hit.zdb_id:
1475085-5
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