In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2023-7-21), p. e0011477-
Abstract:
M . leprae preferentially infects Schwann cells (SCs) in the peripheral nerves leading to nerve damage and irreversible disability. Knowledge of how M . leprae infects and interacts with host SCs is essential for understanding mechanisms of nerve damage and revealing potential new therapeutic strategies. Methodology/Principal findings We performed a time-course single-cell sequencing analysis of SCs infected with M . leprae at different time points, further analyzed the heterogeneity of SCs, subpopulations associated with M . leprae infection, developmental trajectory of SCs and validated by Western blot or flow cytometry. Different subpopulations of SCs exhibiting distinct genetic features and functional enrichments were present. We observed two subpopulations associated with M . leprae infection, a stem cell-like cell subpopulation increased significantly at 24 h but declined by 72 h after M . leprae infection, and an adipocyte-like cell subpopulation, emerged at 72 h post-infection. The results were validated and confirmed that a stem cell-like cell subpopulation was in the early stage of differentiation and could differentiate into an adipocyte-like cell subpopulation. Conclusions/Significance Our results present a systematic time-course analysis of SC heterogeneity after infection by M . leprae at single-cell resolution, provide valuable information to understand the critical biological processes underlying reprogramming and lipid metabolism during M . leprae infection of SCs, and increase understanding of the disease-causing mechanisms at play in leprosy patients as well as revealing potential new therapeutic strategies.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0011477
DOI:
10.1371/journal.pntd.0011477.g001
DOI:
10.1371/journal.pntd.0011477.g002
DOI:
10.1371/journal.pntd.0011477.g003
DOI:
10.1371/journal.pntd.0011477.g004
DOI:
10.1371/journal.pntd.0011477.g005
DOI:
10.1371/journal.pntd.0011477.g006
DOI:
10.1371/journal.pntd.0011477.s001
DOI:
10.1371/journal.pntd.0011477.s002
DOI:
10.1371/journal.pntd.0011477.s003
DOI:
10.1371/journal.pntd.0011477.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2429704-5
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