In:
Journal of Separation Science, Wiley, Vol. 32, No. 13 ( 2009-07), p. 2218-2222
Abstract:
A sensitive HPLC‐fluorescence (FL) method with FL‐labeling reagent for simultaneous determination of 1‐ and 2‐naphthol was developed. The FL‐labeling reaction of naphthols with 4‐(4,5‐diphenyl‐1 H ‐imidazol‐2‐yl)benzoyl chloride (DIB‐Cl) was completed in 20 min at 40°C. The separation of DIB derivatives was achieved with an ODS column and isocratic elution. The LODs of 1‐ and 2‐naphthol were 16 fmol and 14 fmol/injection, respectively, which were 5‐fold and 14‐fold lower than those obtained by HPLC‐FL method using the native FL of naphthols. The DIB‐naphthol derivatives were stable for at least 24 h even when they were placed at room temperature. The proposed method was applied to the measurement of 1‐ and 2‐naphthol in human plasma with sufficient accuracy (90.8–109%) and precision ( 〈 6.3%).
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.200900056
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2047990-6
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