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  • Vulpe, Constantina Bianca  (2)
  • 1
    In: Ecological Chemistry and Engineering S, Walter de Gruyter GmbH, Vol. 30, No. 3 ( 2023-09-01), p. 357-372
    Abstract: The abandoned copper mine in the Moldova Noua area is still producing mining pollution today due to the drying of tailings ponds and the carrying of contaminated dust by the winds, leading to potential contamination of surface waters and groundwaters. The purpose of this study is the assessment of some surface waters and groundwaters potentially contaminated by mining pollutants from Moldova Noua area regarding their hydrochemical and ecotoxicological properties. The physicochemical parameters (pH, conductivity, dissolved oxygen, and turbidity) were determined in situ , with the concentrations of heavy metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) being determined in the laboratory. Inhibition of acid phosphatase enzymatic activity and yeast toxicity tests were the two ecotoxicological tests used for the evaluation of mining pollution effects on the quality of surface waters and groundwater. The results highlighted that most of the physicochemical parameters and heavy metal concentrations were within normal limits. With the exception of the surface water sample collected from the Bosneag River, upstream of Moldova Noua, all samples induced inhibition compared to the positive control in the ecotoxicological tests applied. Statistically significant correlations were observed, with the total concentration of iron ions presenting the most statistically significant correlations with other parameters. A grading scale was used to assess the state of the waters, highlighting the weak impact of mining pollutants on the analysed samples.
    Type of Medium: Online Resource
    ISSN: 2084-4549
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2023
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  • 2
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 24, No. 8 ( 2023-04-20), p. 7575-
    Abstract: The aim of this research was to investigate the bioremediation conditions of copper in synthetic water. In the present study, copper ions accumulation efficiency was determined using various genetically modified strains of Saccharomyces cerevisiae (EBY100, INVSc1, BJ5465, and GRF18), Pichia pastoris (X-33, KM71H), Escherichia coli (XL10 Gold, DH5α, and six types of BL21 (DE3)), and Escherichia coli BL21 (DE3) OverExpress expressing two different peroxidases. Viability tests of yeast and bacterial strains showed that bacteria are viable at copper concentrations up to 2.5 mM and yeasts up to 10 mM. Optical emission spectrometry with inductively coupled plasma analysis showed that the tolerance of bacterial strains on media containing 1 mM copper was lower than the tolerance of yeast strains at the same copper concentration. The E. coli BL21 RIL strain had the best copper accumulation efficiency (4.79 mg/L of culture normalized at an optical density of 1.00), which was 1250 times more efficient than the control strain. The yeast strain S. cerevisiae BJ5465 was the most efficient in copper accumulation out of a total of six yeast strains used, accumulating over 400 times more than the negative control strain. In addition, E. coli cells that internally expressed recombinant peroxidase from Thermobifida fusca were able to accumulate 400-fold more copper than cells that produced periplasmic recombinant peroxidases.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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