In:
FEBS Letters, Wiley, Vol. 351, No. 1 ( 1994-08-29), p. 49-52
Abstract:
The Streptomyces R61 DD‐peptidase gene encodes a 26‐residue C‐terminal extension which is not found in the mature protein. When the gene was expressed in Escherichia coli , the extension was not cleaved and the precursor protein was not enzymatically active. It also reacted with penicillins significantly more slowly than the mature protein. The introduction of a ‘stop’ codon after that corresponding to the C‐terminal residue of the mature protein resulted in the production of an active protein in the periplasm of E. coli .
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)00822-1
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12
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