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  • 1
    Online Resource
    Online Resource
    Biofilm formation by Paracoccus denitrificans requires a type I secretion system-dependent adhesin BapA
    Oxford University Press (OUP) ; 2017
    In:  FEMS Microbiology Letters Vol. 364, No. 4 ( 2017-02-01)
    Details
    In: FEMS Microbiology Letters, Oxford University Press (OUP), Vol. 364, No. 4 ( 2017-02-01)
    Type of Medium: Online Resource
    ISSN: 1574-6968
    URL: Article
    DOI: 10.1093/femsle/fnx029
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2017
    detail.hit.zdb_id: 1501716-3
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  • 2
    Online Resource
    Online Resource
    A Great Leap forward in Microbial Ecology
    Japanese Society of Microbial Ecology ; 2010
    In:  Microbes and Environments Vol. 25, No. 4 ( 2010), p. 230-240
    Details
    In: Microbes and Environments, Japanese Society of Microbial Ecology, Vol. 25, No. 4 ( 2010), p. 230-240
    Type of Medium: Online Resource
    ISSN: 1342-6311 , 1347-4405
    URL: Article
    DOI: 10.1264/jsme2.ME10178
    Language: English
    Publisher: Japanese Society of Microbial Ecology
    Publication Date: 2010
    detail.hit.zdb_id: 2039087-7
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Environmental factors that shape biofilm formation
    Informa UK Limited ; 2016
    In:  Bioscience, Biotechnology, and Biochemistry Vol. 80, No. 1 ( 2016-01-02), p. 7-12
    Details
    In: Bioscience, Biotechnology, and Biochemistry, Informa UK Limited, Vol. 80, No. 1 ( 2016-01-02), p. 7-12
    Abstract: Cells respond to the environment and alter gene expression. Recent studies have revealed the social aspects of bacterial life, such as biofilm formation. Biofilm formation is largely affected by the environment, and the mechanisms by which the gene expression of individual cells affects biofilm development have attracted interest. Environmental factors determine the cell’s decision to form or leave a biofilm. In addition, the biofilm structure largely depends on the environment, implying that biofilms are shaped to adapt to local conditions. Second messengers such as cAMP and c-di-GMP are key factors that link environmental factors with gene regulation. Cell-to-cell communication is also an important factor in shaping the biofilm. In this short review, we will introduce the basics of biofilm formation and further discuss environmental factors that shape biofilm formation. Finally, the state-of-the-art tools that allow us investigate biofilms under various conditions are discussed.
    Type of Medium: Online Resource
    ISSN: 0916-8451 , 1347-6947
    URL: Article
    DOI: 10.1080/09168451.2015.1058701
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2016
    detail.hit.zdb_id: 2110940-0
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    The 〈i〉Pseudomonas〈/i〉 Quinolone Signal Inhibits Biofilm Development of 〈i〉Streptococcus mutans〈/i〉
    Japanese Society of Microbial Ecology ; 2015
    In:  Microbes and Environments Vol. 30, No. 2 ( 2015), p. 189-191
    Details
    In: Microbes and Environments, Japanese Society of Microbial Ecology, Vol. 30, No. 2 ( 2015), p. 189-191
    Type of Medium: Online Resource
    ISSN: 1342-6311 , 1347-4405
    URL: Article
    DOI: 10.1264/jsme2.ME14140
    Language: English
    Publisher: Japanese Society of Microbial Ecology
    Publication Date: 2015
    detail.hit.zdb_id: 2039087-7
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in 〈i〉Alcanivorax borkumensis〈/i〉 SK2
    Japanese Society of Microbial Ecology ; 2019
    In:  Microbes and Environments Vol. 34, No. 1 ( 2019), p. 104-107
    Details
    In: Microbes and Environments, Japanese Society of Microbial Ecology, Vol. 34, No. 1 ( 2019), p. 104-107
    Type of Medium: Online Resource
    ISSN: 1342-6311 , 1347-4405
    URL: Article
    DOI: 10.1264/jsme2.ME18151
    Language: English
    Publisher: Japanese Society of Microbial Ecology
    Publication Date: 2019
    detail.hit.zdb_id: 2039087-7
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  • 6
    Online Resource
    Online Resource
    Outer Membrane Machinery and Alginate Synthesis Regulators Control Membrane Vesicle Production in Pseudomonas aeruginosa
    American Society for Microbiology ; 2009
    In:  Journal of Bacteriology Vol. 191, No. 24 ( 2009-12-15), p. 7509-7519
    Details
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 191, No. 24 ( 2009-12-15), p. 7509-7519
    Abstract: The opportunistic human bacterial pathogen Pseudomonas aeruginosa produces membrane vesicles (MVs) in its surrounding environment. Several features of the P. aeruginosa MV production mechanism are still unknown. We previously observed that depletion of Opr86, which has a role in outer membrane protein (OMP) assembly, resulted in hypervesiculation. In this study, we showed that the outer membrane machinery and alginate synthesis regulatory machinery are closely related to MV production in P. aeruginosa . Depletion of Opr86 resulted in increased expression of the periplasmic serine protease MucD, suggesting that the accumulation of misfolded OMPs in the periplasm is related to MV production. Indeed, the mucD mutant showed a mucoid phenotype and the mucD mutation caused increased MV production. Strains with the gene encoding alginate synthetic regulator AlgU, MucA, or MucB deleted also caused altered MV production. Overexpression of either MucD or AlgW serine proteases resulted in decreased MV production, suggesting that proteases localized in the periplasm repress MV production in P. aeruginosa . Deletion of mucD resulted in increased MV proteins, even in strains with mutations in the Pseudomonas quinolone signal (PQS), which serves as a positive regulator of MV production. This study suggests that misfolded OMPs may be important for MV production, in addition to PQS, and that these regulators act in independent pathways.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    URL: Article
    DOI: 10.1128/JB.00722-09
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 1481988-0
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Autolysis‐mediated membrane vesicle formation in Bacillus subtilis
    Wiley ; 2021
    In:  Environmental Microbiology Vol. 23, No. 5 ( 2021-05), p. 2632-2647
    Details
    In: Environmental Microbiology, Wiley, Vol. 23, No. 5 ( 2021-05), p. 2632-2647
    Abstract: It is known that Bacillus subtilis releases membrane vesicles (MVs) during the SOS response, which is associated with cell lysis triggered by the PBSX prophage‐encoded cell‐lytic enzymes XhlAB and XlyA. In this study, we demonstrate that MVs are released under various stress conditions: sucrose fatty acid ester (SFE; surfactant) treatment, cold shock, starvation, and oxygen deficiency. B. subtilis possesses four major host‐encoded cell wall‐lytic enzymes (autolysins; LytC, LytD, LytE, and LytF). Deletions of the autolysin genes abolished autolysis and the consequent MV production under these stress conditions. In contrast, deletions of xhlAB and xlyA had no effect on autolysis‐triggered MV biogenesis, indicating that autolysis is a novel and prophage‐independent pathway for MV production in B. subtilis . Moreover, we found that the cell lysis induced by the surfactant treatment was effectively neutralized by the addition of exogenous purified MVs. This result suggests that the MVs can serve as a decoy for the cellular membrane to protect the living cells in the culture from membrane damage by the surfactant. Our results indicate a positive effect of B. subtilis MVs on cell viability and provide new insight into the biological importance of the autolysis phenomenon in B. subtilis .
    Type of Medium: Online Resource
    ISSN: 1462-2912 , 1462-2920
    URL: Issue
    URL: Article
    DOI: 10.1111/emi.v23.5
    DOI: 10.1111/1462-2920.15502
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2020213-1
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Table_1.DOCX
    Frontiers Media SA ; 2021
    In:  Frontiers in Microbiology Vol. 12 ( 2021-5-21)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 12 ( 2021-5-21)
    Abstract: Bacterial outer membrane vesicles (OMVs) are spherical lipid bilayer nanostructures released by bacteria that facilitate oral biofilm formation via cellular aggregation and intercellular communication. Recent studies have revealed that Capnocytophaga ochracea is one of the dominant members of oral biofilms; however, their potential for OMV production has yet to be investigated. This study demonstrated the biogenesis of OMVs in C. ochracea associated with the concentration of unsaturated fatty acids of phosphatidylinositol (PI) and characterized the size and protein profile of OMVs produced at growth phases. Transmission electron microscopy showed isolated spherical structures from cells stained with heavy metals, indicating the production of OMVs with a size ranging from 25 to 100 nm. Lipidome analysis revealed the presence of phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine, and PI as the main lipids. Some unsaturated fatty acids of PI were present specifically in OMV and little in the outer membrane, suggesting that OMVs are generated from a specific region of the membrane through blebbing rather than a random process such as cell lysis. Furthermore, the lack of similar PI accumulation in the OMV of Porphyromonas gingivalis suggests that C. ochracea has a different biogenesis mechanism. The blebbing mechanism was further supported by higher OMV production occurring at the exponential phase in comparison to the stationary phase, where cell lysis is more likely to occur. Further, comparative protein profile of OMVs isolated under different growth phases may indicate that the OMV cargo does not largely vary with growth phases. The present study provides a basis for further understanding the roles of C. ochracea OMVs in oral biofilms as well as systemic diseases that C. ochracea involves.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2021.682685
    DOI: 10.3389/fmicb.2021.682685.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2587354-4
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  • 9
    Online Resource
    Online Resource
    Physical Properties and Shifting of the Extracellular Membrane Vesicles Attached to Living Bacterial Cell Surfaces
    American Society for Microbiology ; 2022
    In:  Microbiology Spectrum Vol. 10, No. 6 ( 2022-12-21)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 10, No. 6 ( 2022-12-21)
    Abstract: Bacterial cells release nanometer-sized extracellular membrane vesicles (MVs) to deliver cargo molecules for use in mediating various biological processes. However, the detailed processes of transporting these cargos from MVs to recipient cells remain unclear because of the lack of imaging techniques to image nanometer-sized fragile vesicles in a living bacterial cell surface. Herein, we quantitatively demonstrated that the direct binding of MV to the cell surface significantly promotes hydrophobic quorum-sensing signal (C16-HSL) transportation to the recipient cells. Moreover, we analyzed the MV-binding process in the Paracoccus denitrificans cell surface using high-speed atomic force microscopy phase imaging. Although MV shapes were unaltered after binding to the cell surface, the physical properties of a group of single MV particles were shifted. Additionally, the phase shift values of MVs were higher than that of the cell’s surfaces upon binding, whereas the phase shift values of the group of MVs were decreased during observation. The shifting physical properties occurred irreversibly only once for each MV during the observations. The decreasing phase shift values indicated alterations of chemical components in the MVs as well, thereby suggesting the dynamic process in which single MV particles deliver their hydrophobic cargo into the recipient cell. IMPORTANCE Compared to the increasing knowledge about MV release mechanisms from donor cells, the mechanism by which recipient cells receive cargo from MVs remains unknown. Herein, we have successfully imaged single MV-binding processes in living bacterial cell surfaces. Accordingly, we confirmed the shift in the MV hydrophobic properties after landing on the cell surface. Our results showed the detailed states and the attaching process of a single MV into the cell surface and can aid the development of a new model for MV reception into Gram-negative bacterial cell surfaces. The insight provided by this study is significant for understanding MV-mediated cell-cell communication mechanisms. Moreover, the AFM technique presented for nanometer-scaled mapping of dynamic physical properties alteration on a living cell could be applied for the analyses of various biological phenomena occurring on the cell surface, and it gives us a new view into the understanding of the phenotypes of the bacterial cell surface.
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.02165-22
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2022
    detail.hit.zdb_id: 2807133-5
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  • 10
    Online Resource
    Online Resource
    Influence of the Pseudomonas Quinolone Signal on Denitrification in Pseudomonas aeruginosa
    American Society for Microbiology ; 2008
    In:  Journal of Bacteriology Vol. 190, No. 24 ( 2008-12-15), p. 7947-7956
    Details
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 190, No. 24 ( 2008-12-15), p. 7947-7956
    Abstract: Denitrification is a well-studied respiratory system that is also important in the biogeochemical nitrogen cycle. Environmental signals such as oxygen and N-oxides have been demonstrated to regulate denitrification, though how denitrification is regulated in a bacterial community remains obscure. Pseudomonas aeruginosa is a ubiquitous bacterium that controls numerous genes through cell-to-cell signals. The bacterium possesses at least two N -acyl- l -homoserine lactone (AHL) signals. In our previous study, these quorum-sensing signals controlled denitrification in P. aeruginosa . In addition to the AHL signals, a third cell-to-cell communication signal, 2-heptyl-3-hydroxy-4-quinolone, referred to as the Pseudomonas quinolone signal (PQS), has been characterized. In this study, we examined the effect of PQS on denitrification to obtain more insight into the respiratory regulation in a bacterial community. Denitrification in P. aeruginosa was repressed by PQS, which was partially mediated by PqsR and PqsE. Measuring the denitrifying enzyme activities indicated that nitrite reductase activity was increased by PQS, whereas PQS inhibited nitric oxide reductase and the nitrate-respiratory chain activities. This is the first report to demonstrate that PQS influences enzyme activities, suggesting this effect is not specific to P. aeruginosa . Furthermore, when iron was supplied to the PQS-added medium, denitrifying activity was almost restored, indicating that the iron chelating property of PQS affected denitrification. Thus, our data indicate that PQS regulates denitrification primarily through iron chelation. The PQS effect on denitrification was relevant in a condition where oxygen was limited and denitrification was induced, suggesting its role in controlling denitrification where oxygen is present.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    URL: Article
    DOI: 10.1128/JB.00968-08
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2008
    detail.hit.zdb_id: 1481988-0
    SSG: 12
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