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  • Wiley  (106)
  • Tian, Xin  (106)
  • 1
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    Identification and functional characterization of a novel transglutaminase 1 gene mutation associated with autosomal recessive congenital ichthyosis
    Wiley ; 2016
    In:  International Journal of Dermatology Vol. 55, No. 2 ( 2016-02), p. 201-207
    Details
    In: International Journal of Dermatology, Wiley, Vol. 55, No. 2 ( 2016-02), p. 201-207
    Abstract: Autosomal recessive congenital ichthyosis ( ARCI ) is a group of genetically heterogeneous diseases. Mutations in transglutaminase ( TG ase) 1 gene ( TGM 1, OMIM 190195) have been implicated in ARCI . However, little is known about TGM 1 mutations in the Chinese population, and no functional studies have investigated the biological effect of mutant TGM1 on human epidermal keratinocytes (HaCaT) cells. Objectives To identify the pathogenic mutations of TGM 1 gene in two Chinese siblings with ARCI and gain insight into functional consequences of these mutations. Methods Fifteen exons and flanking splice sites of TGM1 gene were amplified by polymerase chain reaction and then underwent bidirectional Sanger sequencing. The HaCaT cells were transfected with lentiviral vectors, which overexpressed either wild‐type or mutant TGM1 cDNA s with deleted homeodomain. Cell proliferation and cell cycle progression were detected. The expression of cyclin D1, cyclin B1, CDK4, TGM1, K10, involucrin, and filaggrin proteins were investigated by Western blot analysis. Results We found two compound heterozygous missense mutations (c.515C 〉 T, R143C in exon 3 and c.759C 〉 T, S212F in exon 4) in both siblings. HaCaT cells transfected with mutant TGM1 cDNA s displayed a lower growth rate and delayed S phase while overexpression of wild‐type TGM1 cDNA s led to accelerated growth. HaCaT cells transfected with mutant TGM1 cDNA s displayed lower expression of differentiation markers such as involucrin and filaggrin. Our findings suggest that the compound heterozygous missense (c.515C 〉 T, R143C) mutations in exon 3 and missense (c.759C 〉 T, S212F) mutations in exon 4 result in the phenotype of ARCI. TGM1 mutations can suppress keratinocyte growth and cornified cell envelope formation.
    Type of Medium: Online Resource
    ISSN: 0011-9059 , 1365-4632
    URL: Issue
    URL: Article
    DOI: 10.1111/ijd.2016.55.issue-2
    DOI: 10.1111/ijd.12806
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2020365-2
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  • 2
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    Epigenetic landscape of the TERT promoter: a potential biomarker for high risk AML / MDS
    Wiley ; 2016
    In:  British Journal of Haematology Vol. 175, No. 3 ( 2016-11), p. 427-439
    Details
    In: British Journal of Haematology, Wiley, Vol. 175, No. 3 ( 2016-11), p. 427-439
    Abstract: Although recent observations implicate the importance of telomerase activity in acute myeloid leukaemia ( AML ), the roles of epigenetic regulations of the TERT gene in leukaemogenesis, drug resistance and clinical prognosis in AML are not fully understood. We developed a quantitative pyrosequencing‐based methylation assay covering the TERT proximal promoter and a partial exon 1 ( TERT pro/Ex1) region and tested both cell lines and primary leukaemia cells derived from AML and AML with preceding myelodysplastic syndrome ( AML / MDS ) patients ( n  = 43). Prognostic impact of methylation status of the upstream TERT promoter region was assessed by the Kaplan–Meier method. The activity of the telomerase inhibitor, imetelstat, was measured using leukaemia cell lines. The TERT pro/Ex1 region was highly methylated in all cell lines and primary leukaemia cells showed diverse methylation profiles. Most cases showed hypermethylated regions at the upstream TERT pro/Ex1 region, which were associated with inferior patient survival. TERT pro/Ex1 methylation status was correlated with the cytotoxicity to imetelstat and its combination with hypomethylating agent enhanced the cytotoxicity of imetelstat. AML cell lines and primary blasts harbour distinct TERT pro/Ex1 methylation profiles that could serve as a prognostic biomarker of AML . However, validation in a large cohort of patients is necessary to confirm our findings.
    Type of Medium: Online Resource
    ISSN: 0007-1048 , 1365-2141
    URL: Issue
    URL: Article
    DOI: 10.1111/bjh.2016.175.issue-3
    DOI: 10.1111/bjh.14244
    RVK:
    XA 34100
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1475751-5
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  • 3
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    Exercise ameliorating myocardial injury in type 2 diabetic rats by inhibiting excessive mitochondrial fission involving increased irisin expression and AMP‐activated protein kinase phosphorylation
    Wiley ; 2023
    In:  Journal of Diabetes
    Details
    In: Journal of Diabetes, Wiley
    Abstract: Though exercise generates beneficial effects on diabetes‐associated cardiac damage, the underlying mechanism is largely unclear. Therefore, we prescribed a program of 8‐week treadmill training for type 2 diabetes mellitus (T2DM) rats and determined the role of irisin signaling, via interacting with AMP‐activated protein kinase (AMPK), in mediating the effects of exercise on myocardial injuries and mitochondrial fission. Methods Forty 8‐week‐old male Wistar rats were randomly divided into groups of control (Con), diabetes mellitus (DM), diabetes plus exercise (Ex), and diabetes plus exercise and Cyclo RGDyk (ExRg). Ex and ExRg rats received 8 weeks of treadmill running, and the rats in the ExRg group additionally were treated with a twice weekly injection of Cyclo RGDyk, an irisin receptor‐αV/β5 antagonist. At the end of the experiment, murine blood samples and heart tissues were collected and analyzed with methods of ELISA, Western blot, real‐time quantitative polymerase chain reaction, as well as immunofluorescence staining. Results Exercise effectively mitigated T2DM‐related hyperglycemia, hyperinsulinemia, lipid dysmetabolism, and inflammation, which could be diminished by Cyclo RGDyk treatment. Additionally, exercise alleviated T2DM‐induced myocardial injury and excessive mitochondrial fission, whereas the beneficial effects were blocked by the administration of Cyclo RGDyk. T2DM significantly decreased serum irisin concentrations and fibronectin type III domain‐containing protein 5 (FNDC5)/irisin gene and protein expression levels in the rat heart, whereas exercise could rescue T2DM‐reduced FNDC5/irisin expression. Blocking irisin receptor signaling diminished the exercise‐alleviated mitochondrial fission protein expression and elevated AMPK phosphorylation. Conclusion Exercise is effective in mitigating diabetes‐related insulin resistance, metabolic dysfunction, and inflammation. Irisin signaling engages in exercise‐associated beneficial effects on myocardial injury and excessive mitochondrial fission in diabetes rats involving elevated AMPK phosphorylation.
    Type of Medium: Online Resource
    ISSN: 1753-0393 , 1753-0407
    URL: Article
    DOI: 10.1111/1753-0407.13475
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2485432-3
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  • 4
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    PA28γ induces dendritic cell maturation and activates T‐cell immune responses in oral lichen planus
    Wiley ; 2024
    In:  MedComm Vol. 5, No. 5 ( 2024-05)
    Details
    In: MedComm, Wiley, Vol. 5, No. 5 ( 2024-05)
    Abstract: Oral lichen planus (OLP) is a common chronic inflammatory disease of the oral mucosa, the mechanism of its inflammatory progression has not yet been fully elucidated. PA28γ plays a significant role in a variety of immune‐related diseases. However, the exact role of PA28γ in the pathogenesis of OLP remains unclear. Here, we demonstrated that PA28γ is overexpressed in epithelial cells and inflammatory cells of OLP tissues but has no significant relationship with OLP subtypes. Functionally, keratinocytes with high PA28γ expression could induce dendritic cell (DC) maturation and promote the T‐cell differentiation into Th1 cells in response to the immune response. In addition, we found that a high level of PA28γ expression is associated with high numbers of infiltrating mature DCs and activated T‐cells in OLP tissues. Mechanistically, keratinocytes with high PA28γ expression could promote the secretion of C–C motif chemokine (CCL)5, blocking CCL5 or/and its receptor CD44 could inhibit the induction of T‐cell differentiation by keratinocytes with high PA28γ expression. In conclusion, we reveal that keratinocytes with high expression of PA28γ in OLP can induce DC maturation and promote T‐cell differentiation through the CCL5‐CD44 pathway, providing previously unidentified mechanistic insights into the mechanism of inflammatory progression in OLP.
    Type of Medium: Online Resource
    ISSN: 2688-2663 , 2688-2663
    URL: Issue
    URL: Article
    DOI: 10.1002/mco2.v5.5
    DOI: 10.1002/mco2.561
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 3021470-1
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  • 5
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    Nanoparticle‐Induced Exosomes Target Antigen‐Presenting Cells to Initiate Th1‐Type Immune Activation
    Wiley ; 2012
    In:  Small Vol. 8, No. 18 ( 2012-09-24), p. 2841-2848
    Details
    In: Small, Wiley, Vol. 8, No. 18 ( 2012-09-24), p. 2841-2848
    Abstract: The mechanisms associated with the induction of systemic immune responses by nanoparticles are not fully understood, but their elucidation is critical to address safety issues associated with the broader medical application of nanotechnology. In this study, a key role of nanoparticle‐induced exosomes (extracellularly secreted membrane vesicles) as signaling mediators in the induction of T helper cell type 1 (Th1) immune activation is demonstrated. In vivo exposure to magnetic iron oxide nanoparticles (MIONs) results in significant exosome generation in the alveolar region of Balb/c mice. These act as a source of nanoparticle‐induced, membrane‐bound antigen/signaling cargo, which transfer their components to antigen‐presenting cells (APCs) in the reticuloendothelial system. Through exosome‐initiated signals, immature dendritic cells (iDCs) undergo maturation and differentiation to the DC1 subtype, while macrophages go through classical activation and differentiation to the M1 subtype. Simultaneously, iDCs and macrophages release various Th1 cytokines (including interleukin‐12 and tumor necrosis factor α ) driving T‐cell activation and differentiation. Activated APCs (especially DC1 and M1 subtypes) consequently prime T‐cell differentiation towards a Th1 subtype, thereby resulting in an orchestrated Th1‐type immune response. Th1‐polarized immune activation is associated with delayed‐type hypersensitivity, which might underlie the long‐term inflammatory effects frequently associated with nanoparticle exposure. These studies suggest that nanoparticle‐induced exosomes provoke the immune activation and inflammatory responses that can accompany nanoparticle exposure.
    Type of Medium: Online Resource
    ISSN: 1613-6810 , 1613-6829
    URL: Issue
    URL: Article
    DOI: 10.1002/smll.v8.18
    DOI: 10.1002/smll.201200381
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2168935-0
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  • 6
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    TRPV1 activation alleviates cognitive and synaptic plasticity impairments through inhibiting AMPAR endocytosis in APP23/PS45 mouse model of Alzheimer’s disease
    Wiley ; 2020
    In:  Aging Cell Vol. 19, No. 3 ( 2020-03)
    Details
    In: Aging Cell, Wiley, Vol. 19, No. 3 ( 2020-03)
    Abstract: Alzheimer's disease (AD) is one of the most common causes of neurodegenerative diseases in the elderly. The accumulation of amyloid‐β (Aβ) peptides is one of the pathological hallmarks of AD and leads to the impairments of synaptic plasticity and cognitive function. The transient receptor potential vanilloid 1 (TRPV1), a nonselective cation channel, is involved in synaptic plasticity and memory. However, the role of TRPV1 in AD pathogenesis remains largely elusive. Here, we reported that the expression of TRPV1 was decreased in the brain of APP23/PS45 double transgenic AD model mice. Genetic upregulation of TRPV1 by adeno‐associated virus (AAV) inhibited the APP processing and Aβ deposition in AD model mice. Meanwhile, upregulation of TRPV1 ameliorated the deficits of hippocampal CA1 long‐term potentiation (LTP) and spatial learning and memory through inhibiting GluA2‐containing α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor (AMPAR) endocytosis. Furthermore, pharmacological activation of TRPV1 by capsaicin (1 mg/kg, i.p.), an agonist of TRPV1, dramatically reversed the impairments of hippocampal CA1 LTP and spatial learning and memory in AD model mice. Taken together, these results indicate that TRPV1 activation effectively ameliorates cognitive and synaptic functions through inhibiting AMPAR endocytosis in AD model mice and could be a novel molecule for AD treatment.
    Type of Medium: Online Resource
    ISSN: 1474-9718 , 1474-9726
    URL: Issue
    URL: Article
    DOI: 10.1111/acel.v19.3
    DOI: 10.1111/acel.13113
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2099130-7
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  • 7
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    The pathological risk score: A new deep learning‐based signature for predicting survival in cervical cancer
    Wiley ; 2023
    In:  Cancer Medicine Vol. 12, No. 2 ( 2023-01), p. 1051-1063
    Details
    In: Cancer Medicine, Wiley, Vol. 12, No. 2 ( 2023-01), p. 1051-1063
    Abstract: To develop and validate a deep learning‐based pathological risk score (RS) with an aim of predicting patients' prognosis to investigate the potential association between the information within the whole slide image (WSI) and cervical cancer prognosis. Methods A total of 251 patients with the International Federation of Gynecology and Obstetrics (FIGO) Stage IA1–IIA2 cervical cancer who underwent surgery without any preoperative treatment were enrolled in this study. Both the clinical characteristics and WSI of each patient were collected. To construct a prognosis‐associate RS, high‐dimensional pathological features were extracted using a convolutional neural network with an autoencoder. With the score threshold selected by X‐tile, Kaplan–Meier survival analysis was applied to verify the prediction performance of RS in overall survival (OS) and disease‐free survival (DFS) in both the training and testing datasets, as well as different clinical subgroups. Results For the OS and DFS prediction in the testing cohort, RS showed a Harrell's concordance index of higher than 0.700, while the areas under the curve (AUC) achieved up to 0.800 in the same cohort. Furthermore, Kaplan–Meier survival analysis demonstrated that RS was a potential prognostic factor, even in different datasets or subgroups. It could further distinguish the survival differences after clinicopathological risk stratification. Conclusion In the present study, we developed an effective signature in cervical cancer for prognosis prediction and patients' stratification in OS and DFS.
    Type of Medium: Online Resource
    ISSN: 2045-7634 , 2045-7634
    URL: Issue
    URL: Article
    DOI: 10.1002/cam4.v12.2
    DOI: 10.1002/cam4.4953
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2659751-2
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  • 8
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    Sliding window energy detection for spectrum sensing under low SNR conditions
    Wiley ; 2016
    In:  Wireless Communications and Mobile Computing Vol. 16, No. 12 ( 2016-08-25), p. 1654-1663
    Details
    In: Wireless Communications and Mobile Computing, Wiley, Vol. 16, No. 12 ( 2016-08-25), p. 1654-1663
    Abstract: For spectrum sensing, energy detection has the advantages of low complexity, rapid analysis, and requires no knowledge of the transmission signal, which makes it suitable for a wide range of applications. However, under low signal‐to‐noise ratio conditions, the required window length (or the time‐bandwidth product) for energy detection to achieve a desired detection performance is large. In addition, conventional energy detection assumes that the detection tests are independent, that is, there is no overlap between individual detection tests. These properties significantly reduce the detection speed when energy detection is used for the continuous monitoring over a communication channel for the detection of signal transmission activities. In this paper, we propose a sliding window detection analysis with overlap among multiple tests. Algorithms for effective performance analysis of the proposed sliding window energy detection are proposed. The impact of window length on distribution of detection time is investigated. Simulation results on the proposed sliding window energy detection are also compared with the theoretically predicted and conventional energy detection performance estimates. Copyright © 2015 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1530-8669 , 1530-8677
    URL: Issue
    URL: Article
    DOI: 10.1002/wcm.v16.12
    DOI: 10.1002/wcm.2639
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2045240-8
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  • 9
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    Oscillatory haematopoiesis in adults with sickle cell disease treated with hydroxycarbamide
    Wiley ; 2015
    In:  British Journal of Haematology Vol. 168, No. 5 ( 2015-03), p. 737-746
    Details
    In: British Journal of Haematology, Wiley, Vol. 168, No. 5 ( 2015-03), p. 737-746
    Abstract: Hydroxycarbamide therapy has been associated with significant oscillations in peripheral blood counts from myeloid, lymphoid and erythroid lineages in patients with polycythaemia vera and chronic myeloid leukaemia. We retrospectively evaluated serial blood counts over an 8‐year period from 44 adult patients with sickle cell disease receiving hydroxycarbamide. Platelet counts, leucocyte counts, haemoglobin values and reticulocyte counts, apportioned by hydroxycarbamide status, were analysed using a Lomb‐Scargle periodogram algorithm. Significant periodicities were present in one or more counts in 38 patients receiving hydroxycarbamide for a mean duration of 4·81 years. Platelet and leucocyte counts oscillated in 56·8% and 52·3% of patients, respectively. These oscillations generally became detectable within days of initiating therapy. During hydroxycarbamide therapy, the predominant periods of oscillation were 27 ± 1 d for platelet counts and 15 ± 1 d for leucocyte counts. Despite an absolute decrease in leucocyte and platelet counts during hydroxycarbamide treatment, the amplitudes between nadirs and zeniths remained similar regardless of exposure. Our observations appear consistent with previously proposed models of cyclic haematopoiesis, and document that hydroxycarbamide‐induced oscillations in blood counts are innocuous phenomena not limited to myeloproliferative disorders as described previously. We speculate the known cell cycle inhibitory properties of hydroxycarbamide may accentuate otherwise latent constitutive oscillatory haematopoiesis.
    Type of Medium: Online Resource
    ISSN: 0007-1048 , 1365-2141
    URL: Issue
    URL: Article
    DOI: 10.1111/bjh.2015.168.issue-5
    DOI: 10.1111/bjh.13203
    RVK:
    XA 34100
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1475751-5
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  • 10
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    Comparison of metabolic profiles in aqueous humour of Fuchs' syndrome and presumed viral‐induced anterior uveitis patients
    Wiley ; 2022
    In:  Clinical & Experimental Ophthalmology Vol. 50, No. 9 ( 2022-12), p. 1065-1081
    Details
    In: Clinical & Experimental Ophthalmology, Wiley, Vol. 50, No. 9 ( 2022-12), p. 1065-1081
    Abstract: To investigate and compare the metabolic profiles in the aqueous humour of Han Chinese patients with Fuchs' syndrome and presumed viral‐induced anterior uveitis (PVIAU). Methods The metabolites in the aqueous humour of 20 Fuchs' syndrome patients, 20 PVIAU patients and 20 senile cataract control patients were detected by liquid chromatography with mass spectrometry. Differential metabolites were analysed by Student's t test, multivariate analysis, cluster analysis and correlation analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was applied to explore the potential disrupted metabolic pathways in Fuchs' syndrome and PVIAU. Results Comparisons of metabolic profiles identified 29 differential metabolites between Fuchs' syndrome patients and controls, 36 differential metabolites between PVIAU patients and controls, and 30 differential metabolites between Fuchs' syndrome patients and PVIAU patients. DL‐serine was markedly elevated in Fuchs' syndrome, and 1‐palmitoyl‐sn‐glycero‐3‐phosphocholine in PVIAU. KEGG pathway analysis suggested that the differential metabolites in Fuchs' syndrome compared with control were mostly enriched in central carbon metabolism in cancer, adenosine triphosphate‐binding cassette (ABC) transporters and mineral absorption, while those in PVIAU compared with control were mostly enriched in protein digestion and absorption, biosynthesis of unsaturated fatty acids, and ABC transporters. The metabolic pathways differentially affected in Fuchs' syndrome compared to PVIAU included central carbon metabolism in cancer, protein digestion and absorption and ascorbate and aldarate metabolism. Conclusions In Fuchs' syndrome and PVIAU patients, the aqueous humour exhibited specific metabolic profiles and enriched metabolic pathways, which provides a better understanding of the pathogenesis of Fuchs' syndrome and PVIAU in Han Chinese patients.
    Type of Medium: Online Resource
    ISSN: 1442-6404 , 1442-9071
    URL: Issue
    URL: Article
    DOI: 10.1111/ceo.v50.9
    DOI: 10.1111/ceo.14138
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2094910-8
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